Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Wion, Didier; Barrand, Plana; Dicou, Eleni; Scott, James; Brachet, Philippe

doi:10.1016/0014-5793(85)80837-1

Cited by 53 publications

(22 citation statements)

References 26 publications

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“…5 (left panels), addition of anti-T3 serum to unconditioned medium significantly decreased the T3-induced augmentation of cell growth. The mean decrease was 46 medium for experiments 4 and 5, respectively. Although addition of anti-T3 serum caused a small decrease in DNA in these cultures, the change was not significant statistically.…”

Section: Methodsmentioning

confidence: 87%

L-triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth-hormone-producing cell line.

Miller¹,

Fels²,

Shapiro³

et al. 1987

J. Clin. Invest.

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L-Triiodothyronine (T3) stimulates DNA synthesis and replication of cultured GC cells, a T3-responsive growth hormone (GH)-secreting cell line. To determine whether T3 stimulates secretion of an autocrine growth factor, we compared the growthpromoting activity of medium conditioned by T3-stimulated and T3-depleted cells to that of unconditioned medium. Addition of polyclonal rabbit anti-T3 serum to T3-containing media decreased cellular T3 content by 50-70%. In unconditioned medium, anti-T3 serum decreased T3-induced cell growth and GH production by 40-70%. In conditioned medium, anti-T3 serum also effected a 45-70% decrease in induction of GH secretion but did not attenuate the growth-promoting activity. Growth-promoting activity was not detected in medium conditioned by T3-depleted cells. Thus, conditioned medium from T3-containing GC cell cultures contains growth-promoting activity that is independent of T3. Further, the induction of GC cell growth by T3 may occur, at least in part, by induction of an autocrine growth factor.

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Section: Methodsmentioning

confidence: 87%

L-triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth-hormone-producing cell line.

Miller¹,

Fels²,

Shapiro³

et al. 1987

J. Clin. Invest.

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show abstract

“…Another possibility is that hypothalamic NGF might affect levels of other peptides or hormones present in the hypothalamus [51–55]. Taken together, these studies suggest that hypothalamic NGF levels are responsive to (and modified by) stimuli of a psychological nature, most likely associated with anxiety and fear.…”

Section: Stress Renewed Cns and Adrenal Plasticitymentioning

confidence: 99%

NGF, Brain and Behavioral Plasticity

2012

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Nerve Growth Factor (NGF) was initially studied for its role as a key player in the regulation of peripheral innervations. However, the successive finding of its release in the bloodstream of male mice following aggressive encounters and its presence in the central nervous system led to the hypothesis that variations in brain NGF levels, caused by psychosocial stressor, and the related alterations in emotionality, could be functional to the development of proper strategies to cope with the stressor itself and thus to survive. Years later this vision is still relevant, and the body of evidence on the role of NGF has been strengthened and expanded from trophic factor playing a role in brain growth and differentiation to a much more complex messenger, involved in psychoneuroendocrine plasticity.

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“…In this context, DU145 cells can serve as a model for studying regulation of the hNGF gene. Transcription of the murine NGF gene was shown to be stimulated by serum and thyroid hormone T3 (Wion et al, 1985), retinoic acid (Wion et al, 1987), PMA (Wion et al, 1990), and 1,25-(OH)2D, (Wion et al, 1991) and to be inhibited by dexamethasone (Wion et a]., 1986). We found that NGF synthesis in DU145 cells is constitutive in the absence of serum; however, the addition of serum stimulated hNGF transcription by three-to fourfold, compared with a five-to tenfoId stimulation of murine NGF transcription (Wion et al, 1990).…”

Section: Neurdtemmentioning

confidence: 99%

Expression of Nerve Growth Factor and Nerve Growth Factor Receptor Genes in Human Tissues and in Prostatic Adenocarcinoma Cell Lines

Saint‐André

Dicou

1992

Journal of Neurochemistry

Self Cite

103

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Nerve growth factor (NGF) mRNAs were detected and quantified in a variety of normal and neoplastic human tissues by northern blot hybridization. Human heart contained the highest NGF mRNA levels, whereas lower but comparable levels were found in the placenta, prostate, and kidney. All tissues examined coexpressed the low-affinity NGF receptor (LNGFR), whereas none of these tissues expressed the high-affinity NGF receptor encoded by the trk protooncogene. The widespread distribution of the LNGFR suggests that it plays a role in the regulation of normal cell growth. No overexpression of NGF or LNGFR mRNA was detected in neoplastic tissues, whereas LNGFR-like immunoreactivity was localized outside of tumor cells. Transforming growth factor-alpha and protooncogene c-fos expression in these tissues did not show a systematic correlation with NGF/LNGFR expression. Furthermore, regulation of the human NGF gene was studied in DU145 cells, a prostatic adenocarcinoma cell line that synthesizes significant NGF mRNA levels. Serum induced, whereas dexamethasone inhibited, NGF mRNA synthesis in these cells. Serum induction was preceded by a rapid and transient activation of the c-fos protooncogene.

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Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells

Cited by 53 publications

References 26 publications

L-triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth-hormone-producing cell line.

L-triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth-hormone-producing cell line.

NGF, Brain and Behavioral Plasticity

Expression of Nerve Growth Factor and Nerve Growth Factor Receptor Genes in Human Tissues and in Prostatic Adenocarcinoma Cell Lines

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