Serum anti-rabbit and anti-horse IgG, IgA, and IgM in kidney transplant recipients

Abstract

Serum anti-rabbit and/or -horse antibodies were demonstrated in a significant proportion of kidney recipients, even before transplantation, possibly due to environmental exposure. A classical pattern of IgM increase was observed when the patients developed an immune response to ALG or ATG, and an IgA response after ALG. These results suggest that patients receiving ALG/ATG should be monitored for the production of anti-ALG/ATG immunoglobulins.

In vivo effects of horse and rabbit antithymocyte globulin in patients with severe aplastic anemia

“…Human anti-rATG or hATG antibody titers correlated with the occurrence of SS, the IgM subtype appearing more specific, consistent with previous findings. 9,10 In addition, we did not find any correlation of SS with response to ATG treatment, or with neutrophil and red blood cell counts, but SS was associated with higher platelet counts from week 2 to 6 months ( Figure 6B). …”

Rabbit antithymocyte globulin–induced serum sickness disease and human kidney graft survival

“…Total anti-rabbit IgG and IgM antibodies Quantification of human serum IgGs against anti-rabbit IgGs (Thymoglobulin, Genzyme) was adapted from Prin Mathieu et al (47). Plates (NUNC Maxisorp; NUNC AB) were coated overnight with thymoglobulin (1 μg/ml) in 50 mM sodium carbonate-bicarbonate buffer (pH 9).…”

Lymphodepletional Strategies in Transplantation

“…Although monoclonal OKT-3 solved some of these issues with polyclonal preparations, it was still subject to a significant antimouse response, as OKT3 is an entirely mouse-derived antibody (Webster et al 2006). These xenogeneic antibodies can dramatically reduce the efficacy of these agents and extended/repeated use may lead to anaphylaxis and serum sickness (Prin Mathieu et al 1997;Regan et al 1997Regan et al , 2001). The first dose of these antibodies can be followed by fevers, chills, and gastointestinal, respiratory, and cardiac complications, mostly due to the release of pyrogenic cytokines such as IL-6 and TNF-a from target cell lysis (Debets et al 1989;Chatenoud et al 1990;Vallhonrat et al 1999).…”