Serum Anti-SlOOb, Anti-GFAP and Anti-NGF Autoantibodies of IgG Class in Healthy Persons and Patients with Mental and Neurological Disorders

Abstract

Natural autoantibodies of IgG class to proteins S100b, glial fibrillar acidic protein (GFAP), and nerve growth factor (NGF) are presented in serum of healthy adults and those levels/affinities are relatively constant and may vary among individuals within narrow limits. In patients with depressive disorder, epilepsy, multiple sclerosis, and Parkinson's disease dispersion of such autoantibodies serum levels were often found beyond the normal ranges. Most of the patient groups include cases with significantly elevated as well as abnormally decreased immunoreactivity parameters. This leads us to assumption that changes in some basic mechanisms of individual immune state represent the common features of different forms of pathology of the nervous system.

Dialectics and Implications of Natural Neurotropic Autoantibodies in Neurological Disease and Rehabilitation

“…Cytoplasmic brain protein S100, cytoskeletal glial fibrillary acidic protein (GFAP), and 65-kDa brain cell membrane protein MP65 were purified from bovine brain as previously described (Poletaev et al, 2000). Nerve growth factor (NGF) was purchased from Sigma-Aldrich (Moscow, Russia).…”

“…Study of IR EIA was performed as previously described (Poletaev et al, 2000. Briefly, serum samples were diluted 1:200 in 0.05% Tween 20 PBS (PBST), and 96-well immunoplates (Nunc maxisorp) were coated with Id-or AId-Fab-fragments.…”

“…A highly standardized sample of human immunoglobulins of IgG class was used as control sample and evaluated in each immunoplate in parallel with analyzed serum samples. The Id -AId reactions ratio for each serum sample was calculated as described (Poletaev et al, 2000 by the following equation:…”

“…ðCU þ 100Þ reaction with anti -idiotpic Fab ðCU þ 100Þ reaction with corresponding idiotpic Fab Normal values were considered between 2 35 and 40 CU and between 0.7 and 1.3, respectively, as previously described (Poletaev et al, 2000(Poletaev et al, , 2002.…”

“…Accordingly, disturbances of any of the three components eventually lead to compensatory changes in the activity of the others. As an example, shifts in serum natural autoantibodies (auto-Abs) against nervous cells proteins, such as neurotropic idiotypic auto-Abs (Id-Abs) described in mental and neurological diseases (Poletaev et al, 2000(Poletaev et al, , 2002, have been shown to be associated with shifts in anti-idiotypic auto-Abs (AId-Abs). It is still unknown, however, if changes in auto-Abs titers constitute a non-pathological physiological response to an injurious insult or whether they characterize the pathological process, possibly worsening the disorder.…”