Serum antibodies to collagen type IV and development of diabetic vascular complications in children with type 1 (insulin-dependent) diabetes mellitus

Nicoloff, G.; Baydanoff, S; Petrova, Ch.; Christova, P.

doi:10.1016/s1537-1891(02)00214-8

Cited by 14 publications

(13 citation statements)

References 26 publications

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“…Similar studies on the quantitation of CIC levels have been conducted on a variety of autoimmune disease including systemic sclerosis (Stanilova and Slavov, 2003), SLE and rheumatoid arthritis (Ö lmez et al, 1991;Stanilova and Slavov, 2001). Type 1 diabetes mellitus is also an autoimmune disease in which different types of autoantibodies have been identified such as those against beta cells (insulin-autoantibodies, islet cell-autoantibodies, GAD autoantibodies (Verge et al, 1998)) and antibodies against vascular wall proteins such as elastin (Nicoloff et al, 2000b) and collagen (Nicoloff et al, 2002). Our aim in the studies reported herein was to test the relationship between CIC and vascular complications in diabetic children.…”

Section: Discussionmentioning

confidence: 99%

“…Peptides derived from such processes have been found in the circulation (Nicoloff et al, 2000a(Nicoloff et al, , 2001 and they are reasoned to be a stimulus for increased production of autoantibodies. A variety of autoantibodies have been documented in diabetes and include insulin autoantibodies, GAD-autoantibodies, tyrosine phosphatase IA2-autoantibodies, islet cell-autoantibodies, (Verge et al, 1998) elastin autoantibodies (Nicoloff et al, 2000b) and collagen type IV autoantibodies (Nicoloff et al, 2002). These autoantibodies bind to their cognate antigen and thus form circulating immune complexes (CIC).…”

Section: Introductionmentioning

confidence: 99%

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Circulating Immune Complexes among Diabetic Children

Nicoloff

Blazhev

Petrova³

et al. 2004

Journal of Immunology Research

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Insulin dependent diabetes mellitus (IDDM) is an autoimmune disease associated with the presence of different types of autoantibodies. The presence of these antibodies and the corresponding antigens in the circulation leads to the formation of circulating immune complexes (CIC). CIC are known to persist in the blood for long periods of time. Such CIC following deposition in the small blood vessels have the potential to lead to microangiopathy with debilitating clinical consequences. The aim of our pilot study was to investigate whether a correlation exists between CIC and the development of microvascular complications in diabetic children. Isolation of a new glycoprotein complement inhibition factor (CIF) from the parasitic plant Cuscuta europea seed, which appears to bind specifically to complement component C3 has provided an unique tool for the measurement of immune complexes by means of ELISA-type techniques (CIF-ELISA). We studied the levels of CIC (IgG, IgM and IgA) in 58 diabetic children (mean age 12.28±4.04 years, diabetes duration 5.3±3.7 years), 29 of them had vascular complications (group 1) and the other 29 were without vascular complications (group 2). As controls, we studied sera samples from 21 healthy children (mean age 13.54±4.03 years). Sera from the diabetic patients showed statistically significant higher levels of CIC IgG ( p=0.03) than sera from the control group. In sera from group 1 values of CIC IgG showed statistically significant higher levels than controls (0.720±0.31 vs. 0.46±0.045; p=0.011) Sera from 59% of the patients were positive for CIC IgG, 36% for CIC IgM and 9% for CIC IgA. Among 26 patients with microalbuminuria, sera from 17/26 (65%) were positive for CIC IgG, 8/26 (31%) for CIC IgM and 2/26 (8%) for CIC IgA. CIC IgG correlated with HbA1c (r=0.51; p=0.005) and microalbuminuria (r=0.42, p=0.033). CIC IgA correlated with age (r=0.44, p=0.03). CIC IgM correlated with the duration of diabetes (r=0.63, p=0.02). These findings suggest that elevated levels of CIC IgG are associated with the development of early diabetic nephropathy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Circulating Immune Complexes among Diabetic Children

Nicoloff

Blazhev

Petrova³

et al. 2004

Journal of Immunology Research

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“…Increased levels of autoantibodies against collagen type IV have been associated with diabetes and development of microangiopathy [14] and have also been reported in patients with systemic vasculitis and Crohn's disease [13], [15]. Moreover, autoantibodies against collagen type IV have been detected in children with type 1 diabetes and hypertension [16], [17].…”

Section: Introductionmentioning

confidence: 96%

Autoantibodies against basement membrane collagen type IV are associated with myocardial infarction

McLeod

Dunér

Samnegård

et al. 2015

IJC Heart & Vasculature

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BackgroundCollagen type IV is the major constituent of basement membranes underlying endothelial cells and is important for endothelial cell attachment and function. Autoantibodies against native collagen type IV have been found in various autoimmune diseases. Oxidation of LDL in the vascular wall results in the formation of reactive aldehydes, which could modify surrounding matrix proteins. Like oxidized LDL, these modified matrix proteins are likely to induce immune responses. We examined whether autoantibodies against native or aldehyde-modified collagen type IV are associated with myocardial infarction.MethodsIgM and IgG against native and aldehyde-modified collagen type IV were measured by ELISA in serum from 387 survivors of a first myocardial infarction and 387 age- and sex-matched controls.ResultsPost-infarction patients had significantly increased levels of IgM against native collagen type IV, and IgG against native collagen type IV was present at detectable level in 17% of patients as opposed to 7% of controls (p < 0.001). Controlling for major cardiovascular risk factors demonstrated that the presence of IgG against native collagen type IV was associated with myocardial infarction (OR 2.9 (1.6–5.4), p = 0.001). Similarly, subjects in the highest quartile of IgM against native collagen type IV had increased risk of having suffered myocardial infarction (OR 3.11 (1.8–5.4), p < 0.001) after adjusting for cardiovascular risk factors. In contrast, IgG against aldehyde-modified collagen type IV was decreased in myocardial infarction patients, but this association was not independent of established cardiovascular risk factors.ConclusionAutoantibodies against collagen type IV are associated with myocardial infarction independently of traditional cardiovascular risk factors.

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“…The plate was incubated for 1 h at room temperature in a dark chamber. The reaction was stopped by adding 4 …”

Section: Antigenmentioning

confidence: 99%

Increased elastin turnover in diabetic patients with arterial hypertension

Николов¹,

Tsinlikov²,

Nicoloff³

et al. 2013

cejoi

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0001). Group 1 (patients with microvascular complications) showed a significant increase in free AeAbs igG in comparison with controls: 0.428 (0.343-0.591) vs. 0.240 (0.212-0.305), respectively (kW = 20.14; p < 0.0001). Group 2 also shows higher levels of free AeAbs igG than the control group: 0.398 (0.312-0.467) vs. 0.240 (0.212-0.305), respectively (kW = 8.88; p = 0.003). patients with microvascular complications showed the highest levels of free AeAbs igG. There were no significant differences between group 1 and group 2.Conclusions: our results show the association between the activity of elastin turnover and microvascular lesions in diabetic patients with arterial hypertension. We suggest that free AeAbs igG mark a later "secondary" step of the autoimmunization to elastin.

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Serum antibodies to collagen type IV and development of diabetic vascular complications in children with type 1 (insulin-dependent) diabetes mellitus

Cited by 14 publications

References 26 publications

Circulating Immune Complexes among Diabetic Children

Circulating Immune Complexes among Diabetic Children

Autoantibodies against basement membrane collagen type IV are associated with myocardial infarction

Increased elastin turnover in diabetic patients with arterial hypertension

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