Serum antibodies to enterohepatic Helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of H. pylori infection

Vorobjova, Tamara; Nilsson, Ingrid; Terjajev, S; Granholm, Mallory A; Lyyra, M; Porkka, T; Prükk, Tiina; Salupere, Riina; Maaroos, H I; Wadström, Torkel; Uibo, Raivo

doi:10.1016/j.dld.2005.12.008

Cited by 23 publications

(22 citation statements)

References 28 publications

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“…However, cDNA of H. hepaticus has not yet been identified. On the other hand, serodiagnosis has suggested that H. hepaticus infection may be involved in primary sclerosing cholangitis and other liver diseases [11,12].…”

Section: Introductionmentioning

confidence: 99%

Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen

et al. 2011

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Section: Introductionmentioning

confidence: 99%

Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen

et al. 2011

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“…Then, 10 l of serum was added to 1 ml of cell lysate (with the density adjusted at A 540 to 1.5), followed by incubation for 2.5 h at 22°C under constant shaking. Cells were removed by centrifugation at 12,000 ϫ g for 15 min, and the supernatants were used for the serology (21,49).…”

Section: Methodsmentioning

confidence: 99%

Cross-Reactivity between Immune Responses to Helicobacter bilis and Helicobacter pylori in a Population in Thailand at High Risk of Developing Cholangiocarcinoma

Pisani

Whary

Nilsson

et al. 2008

Clin Vaccine Immunol

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Helicobacter bilis DNA has been detected in human tissue and is a candidate for etiologic investigations on the causes of hepatic and biliary tract diseases, but reliable serologic tests need to be developed in order to pursue such investigations. The scope of this study was to assess the specificity of two assays for H. bilis immune response allowing for H. pylori, and their cross-reactivity in a population in Thailand at high risk for cholangiocarcinoma. Plasma samples from 92 Thai volunteers were independently tested in two laboratories (Massachusetts Institute of Technology [MIT] and Lund). MIT performed three analyses of H. pylori and H. bilis based either on (i) outer

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“…in question have not been identified. (12)(13)(14) This is mainly attributed to the extreme difficulty encountered in the separation of Helicobacter from liver tissues, as well as in the subsequent culture process. The current commonly used molecular biology technique for identifying Helicobacter consists of detecting Helicobacter-specific 16S rRNA genes using polymerase chain reaction (PCR).…”

Section: Since Its Initial Identification H Pylori Infection Has Notmentioning

confidence: 99%

Helicobacter hepaticus infection in primary hepatocellular carcinoma tissue

Yang¹,

Ji²,

Zhang³

et al. 2013

smedj

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INTRODUCTION Helicobacter (H.) hepaticus infection causes chronic active hepatitis and induces hepatocellular tumours in A/JCr mice, but evidence of this in humans is scarce. This study aimed to demonstrate the correlation between H. hepaticus and human primary hepatocellular carcinoma (HCC). MeThODsThe sera of 50 patients with primary HCC were tested for the presence of anti-H. pylori and anti-H. hepaticus immunoglobulin G (IgG) antibodies. The liver tissues of patients who tested positive for serum antibody were analysed for H. hepaticus-specific 16S rRNA, H. hepaticus cdtB, H. pylori cagA, H. pylori vacA and H. pylori ureC genes using polymerase chain reaction. ResUlTsAfter the anti-H. pylori antibodies in the serum samples were absorbed by H. pylori antigen, the anti-H. hepaticus IgG serum antibody detection rate was 50.0% in patients with primary HCC. This was significantly higher (p < 0.001) than the detection rate in the benign liver tumour (7.7%) and normal liver tissue (6.3%) groups. Of the 25 primary HCC samples that tested positive for anti-H. hepaticus IgG serum antibody, the H. hepaticus-specific 16S rRNA gene was detected in nine (36.0%) samples. Sequencing showed that the polymerase chain reaction-amplified product exhibited 95.5%-100% homology to the H. hepaticus-specific 16S rRNA gene. Among these nine primary HCC tissue samples, the H. hepaticus cdtB gene was detected in four (44.4%) samples, while no such expression was observed in the benign liver tumour or normal liver tissue groups. CONClUsIONThe present study identified the presence of H. hepaticus infection in patients with primary HCC using serological and molecular biological detection, suggesting that H. hepaticus infection may be involved in the progression of HCC.

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Serum antibodies to enterohepatic Helicobacter spp. in patients with chronic liver diseases and in a population with high prevalence of H. pylori infection

Cited by 23 publications

References 28 publications

Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen

Serodiagnosis of Helicobacter hepaticus infection in patients with liver and gastrointestinal diseases: western blot analysis and ELISA using a highly specific monoclonal antibody for H. hepaticus antigen

Cross-Reactivity between Immune Responses to Helicobacter bilis and Helicobacter pylori in a Population in Thailand at High Risk of Developing Cholangiocarcinoma

Helicobacter hepaticus infection in primary hepatocellular carcinoma tissue

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