Serum antibody response to filamentous hemagglutinin in patients with clinical pertussis measured by an enzyme-linked immunosorbent assay

Zackrisson, Gunilla; Arminjon, F.; Krantz, Ingela; Lagergård, Teresa; Sigurs, Nele; Taranger, J; Trollfors, Birger

doi:10.1007/bf01975044

Cited by 27 publications

(12 citation statements)

References 14 publications

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“…Several studies have compared the abilities of ELISAs based on different B. pertussis antigens to detect increases in specific antibodies by using paired sera from patients with mostly culture-confirmed pertussis. The ability was similar or slightly superior for Ptx-based ELISAs compared to FHA-based ELISAs (177,179,193,198,206,208,209) and was considerably better for Ptx-based ELISAs than for ELISAs based on Prn, fimbriae (177,198,202,206,208), outer membrane protein extracts, or sonicates of B. pertussis cells (189). In young children with pertussis, IgG responses to fimbriae occurred only in those who had been primed by vaccination with vaccines containing fimbriae (210).…”

Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

“…In studies in which IgM, IgA, and IgG antibodies were measured in sera from patients with well-documented pertussis and with various ages and vaccination histories, the common finding was that the IgG parameters were most sensitive (31,180,182,191,193,194). Likewise, in studies in which IgG and IgA antibodies were measured, IgG parameters were more sensitive than IgA parameters, and combinations of IgG and IgA parameters (with "and/or" interpretation) did not enhance or only slightly enhanced the sensitivity (186,187,(195)(196)(197)(198).…”

Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

“…Cutoff points for significant increases of antibody in paired sera used in various studies included 1.5-fold (17, 187), 2-fold (31,198,201,209,225,228,(235)(236)(237)(238), 3-fold (193,194,208), and 4-fold (9,182). Because of the limited accuracy of ELISA (high CVs) at low values (222), a criterion for the minimal level to be reached in the second serum is included in most definitions for significant dynamics, i.e., minimally 4 times the detection level or, specifically for IgG-Ptx ELISA, minimally 20 CBER EU/ml or 20 WHO IU/ml.…”

Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

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Laboratory Diagnosis of Pertussis

2015

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SUMMARYThe introduction of vaccination in the 1950s significantly reduced the morbidity and mortality of pertussis. However, since the 1990s, a resurgence of pertussis has been observed in vaccinated populations, and a number of causes have been proposed for this phenomenon, including improved diagnostics, increased awareness, waning immunity, and pathogen adaptation. The resurgence of pertussis highlights the importance of standardized, sensitive, and specific laboratory diagnoses, the lack of which is responsible for the large differences in pertussis notifications between countries. Accurate laboratory diagnosis is also important for distinguishing between the several etiologic agents of pertussis-like diseases, which involve both viruses and bacteria. If pertussis is diagnosed in a timely manner, antibiotic treatment of the patient can mitigate the symptoms and prevent transmission. During an outbreak, timely diagnosis of pertussis allows prophylactic treatment of infants too young to be (fully) vaccinated, for whom pertussis is a severe, sometimes fatal disease. Finally, reliable diagnosis of pertussis is required to reveal trends in the (age-specific) disease incidence, which may point to changes in vaccine efficacy, waning immunity, and the emergence of vaccine-adapted strains. Here we review current approaches to the diagnosis of pertussis and discuss their limitations and strengths. In particular, we emphasize that the optimal diagnostic procedure depends on the stage of the disease, the age of the patient, and the vaccination status of the patient.

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Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

Section: Serodiagnosis Of Pertussismentioning

confidence: 99%

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Laboratory Diagnosis of Pertussis

2015

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“…5). FHA is also a potent immunogen, capable of inducing high levels of mucosal and systemic antibodies of long persistence after natural infection in humans (6,7) or experimental infection in mice (8).…”

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confidence: 99%

Induction of mucosal immune responses against a heterologous antigen fused to filamentous hemagglutinin after intranasal immunization with recombinant Bordetella pertussis.

Renauld-Mongénie

Mielcarek

Cornette

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Live vaccine vectors are usually very effective and generally elicit immune responses of higher magnitude and longer duration than nonliving vectors. Consequently, much attention has been turned to the engineering of oral pathogens for the delivery of foreign antigens to the gutassociated lymphoid tissues. However, no bacterial vector has yet been designed to specifically take advantage of the nasal route of mucosal vaccination. Herein we describe a genetic system for the expression of heterologous antigens fused to the filamentous hemagglutinin (FHA) in Bordetella pertussis. The Schistosoma mansoni glutathione S-transferase (Sm28GST) fused to FHA was detected at the cell surface and in the culture supernatants of recombinant B. pertussis. The mouse colonization capacity and autoagglutination of the recombinant microorganism were indistinguishable from those of the wildtype strain. In addition, and in contrast to the wild-type strain, a single intranasal administration of the recombinant strain induced both IgA and IgG antibodies against Sm28GST and against FHA in the bronchoalveolar lavage fluids. No anti-Sm28GST antibodies were detected in the serum, strongly suggesting that the observed immune response was of mucosal origin. This demonstrates, to our knowledge, for the first time that recombinant respiratory pathogens can induce mucosal immune responses against heterologous antigens, and this may constitute a first step toward the development of combined live vaccines administrable via the respiratory route.

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“…Enzyme-linked immunosorbent assays (ELISAs) currently are the method of choice for detection of antibodies to B. pertussis antigens (16). Various ELISA formats with different antigens have been developed (5,8,12,14,23,24,29,30) and were evaluated intensively in vaccine trials (7,11,18,27).…”

mentioning

confidence: 99%

Comparison of Five Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis

Kösters

Riffelmann

Dohrn

et al. 2000

Clin Diagn Lab Immunol

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Measuring antibodies to Bordetella pertussis antigens is mostly done by enzyme-linked immunosorbent assays (ELISAs). We compared the performance of five commercially available ELISA kits with the help of 65 serum specimens which were repetitively tested for evaluation of the kits. The specimens contained 20 paired serum samples from patients with clinical pertussis, 15 samples were from children vaccinated with a diphtheriatetanus-acellular pertussis vaccine, seven specimens were taken from an interlaboratory comparison of ELISAs, and there were three reference preparations from the Food and Drug Administration's (FDA's) Laboratory of Pertussis and from our laboratory. Reference values were obtained from the FDA or from results obtained with an in-house ELISA. Commercial ELISAs were compared with respect to their reproducibility and variability, their ability to detect significant titer rises in paired serum samples, their ability to detect an immune response after vaccination, and the comparability of semiquantitative and quantitative results. Reproducibility was generally good (>89%), intra-assay variation ranged from 2.4 to 28.7%, and indeterminate results were recorded in up to 18.5% of all specimens. Most kits correctly identified the antibody response to an acellular pertussis vaccine. None of the commercial kits identified all cases of pertussis correctly, and the sensitivity ranged between 60 and 95%. All five commercial ELISAs showed great discrepancies when comparing semiquantitative results and contained obviously different antigen preparations. Our data suggest that the five commercial ELISAs tested here need further improvement and standardization.

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Serum antibody response to filamentous hemagglutinin in patients with clinical pertussis measured by an enzyme-linked immunosorbent assay

Cited by 27 publications

References 14 publications

Laboratory Diagnosis of Pertussis

Laboratory Diagnosis of Pertussis

Induction of mucosal immune responses against a heterologous antigen fused to filamentous hemagglutinin after intranasal immunization with recombinant Bordetella pertussis.

Comparison of Five Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis

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