Serum antibody specificities to Leishmania aethiopica antigens in patients with localized and diffuse cutaneous leishmaniasis

Mengistu, Getahun; Akuffo, Hannah; Yemane-Berhan, T; Britton, Sven; Fehniger, Thomas E.

doi:10.1111/j.1365-3024.1990.tb00984.x

Cited by 16 publications

(16 citation statements)

References 13 publications

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“…Iranian patients sera mainly recognized antigens of the 55-115kDa region on LV4 blots, and Brazilian and Bolivian sera primarily identified antigens in the 43-1 15 kDa region. In other studies (Jaffe et al 1990, Mengistu et al 1990, limited recognition in immunoblotting of antigens of < 50 kDa by serum antibodies from localized CL patients have been reported. We did not observe a correlation between the pattern of antibody reactivity observed in immunoblot, and the duration of active infection: for example, two of the patients (Figure 2a: lanes 3 and 5) who have been infected for the same amount of time (seven years) showed varying immunoblot profiles; nor with the number of lesions: for example, two of the patients (Figure 2a: lanes 9 and 11) who presented both four lesions showed different immunoblot profiles.…”

Section: Infanrum Lem 497 Polypeptides Separated By 10% Polyacrylamentioning

confidence: 91%

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Rolland¹,

Zilberfarb

Furtado

et al. 1994

Parasite Immunology

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We have analysed by immunoblotting sera from humans and dogs with visceral leishmaniasis, from the Old World as well as the New. When lysates of promastigotes are used as antigens, antibodies against a 94 kDa Leishmania component are detected, regardless of the age and geographical origin of the patient, the serum antibody titre as measured by indirect immunofluorescence, and the number of arcs in counterimmunoelectrophoresis. Low dilutions of sera from patients with Old and New World cutaneous leishmaniasis did not react with the 94-kDa antigen, whatever the species of Leishmania used as antigens. Sera from patients with other infections than leishmaniases, or without infection, are negative, even at low dilution. Anti-94 kDa antibodies were detected in the sera of Leishmania-infected dogs from both the Old and the New World. When lysates of Leishmania mexicana axenic amastigotes are used as antigens, the 94-kDa antigen was little or none identified by sera from humans and dogs with visceral leishmaniasis, and never recognized by control sera. Thus, the specific recognition of the 94-kDa promastigote antigen in human and canine visceral leishmaniasis suggests that this antigen could be a potential candidate in the differential immunodiagnosis of the disease.

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Section: Infanrum Lem 497 Polypeptides Separated By 10% Polyacrylamentioning

confidence: 91%

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Rolland¹,

Zilberfarb

Furtado

et al. 1994

Parasite Immunology

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“…We.stern blots. Whole promastigote lysates were obtained by solubilizing washed promastigotes in sample buffer as described [14]. The samples were boiled for 5 min and then spun at 6,000f or 5 min to pellet the insoluble material.…”

Section: Methodsmentioning

confidence: 99%

Visceral Leishmaniasis in Ververt Monkeys: Immunological Responses During Asymptomatic Infections

Gicheru¹,

Olobo²,

Kariuki³

et al. 1995

Scand J Immunol

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Nine vervet monkeys (Cercopithecus aethiops) were infected intradermally with 8 x 10(7) virulent L. donovani promastigotes. Four animals developed clinical visceral leishmaniasis and died over a period of 18 months. The remaining five animals have remained asymptomatic for a period of 3 years now. Attempts to isolate parasites from spleen and liver through biopsies were fruitless. Immunological responses of these subclinically infected animals were examined. Enzyme-linked immunosorbent assay (ELISA) and western blot analyses demonstrated Leishmania specific antibodies in these animals, but the antibody titres were low. When proliferation of peripheral blood monocytes (PBMC) to Concanavalin A (Con A) of these animals was compared with control 'disease free animals' there were no significant differences in response. However, L. donovani antigen (fixed promastigotes) specific proliferation was demonstrated in the five subclinically infected animals. High and varying levels of interferon gamma (IFN-gamma) were secreted in PBMC cultures from the five vervet monkeys when stimulated with either Con A or L. donovani antigens. In control animals, IFN-gamma was only detected when PBMC were stimulated with Con A. Marked delayed-type hypersensitivity (DTH) responses were demonstrated in the five subclinically infected animals 48 h after injection with formalin fixed promastgotes. It was concluded that the visceral Leishmania disease spectrum due to L. donovani observed in humans could be induced in vervet monkeys and that L. donovani asymptomatic/cryptic infected animals have competent humoral and cellular responses to homologous parasites.

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“…High antibody levels are present in the more severe clinical form of the cutaneous disease, namely diffuse cutaneous leishmaniasis (DCL) (Schurr et al 1986, Mengistu et al 1990), but B cell depletion does not alter the susceptibility or resistance pattern to Leishmania infection in mice (Babai et al 1999, Brown & Reiner 1999. It seems that B cells are important to induce anti-Leishmania CD4 + Th1 cells and DTH reaction, in the resistant mouse strain, and take part in the humoral response development in susceptible animals (Scott & Farrell 1982, Scott et al 1986).…”

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confidence: 99%

B-cell infiltration and frequency of cytokine producing cells differ between localized and disseminated human cutaneous leishmaniases

Vieira

Oliveira

Arruda

et al. 2002

Mem. Inst. Oswaldo Cruz

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Biopsies from human localized cutaneous lesions (LCL n = 7) or disseminated lesions (DL n = 8) cases were characterized according to cellular infiltration, frequency of cytokine (IFN-γ, TNF-α) Cutaneous leishmaniasis is a worldwide disease with severe deformating potential in new world. It affects preferentially young economically active patients representing a large burden to the public health system in developing countries. Protection against all forms of leishmaniasis is dependent on cell-mediated immunity (CMI), but the contribution of some cells and cytokines in human disease deserves further scrutiny.CD8 + T cells have been implicated in protection (Muller et al. 1991) being high IFN-γ producers in a murine model of leishmaniasis (Chan 1993). Their role seems to be more in the secondary than in the primary immune response (Muller et al. 1993(Muller et al. , 1994. On the other hand, the course of leishmaniasis in mice lacking beta 2-microglobulin (beta 2-m) gene did not differ from their wild-type counterparts (Overath & Harbecke 1993, Wang et al. 1993, Huber et al. 1998) lessening a role of antigen presentation by major histocompatibility complex class I (MHC I) molecules. In man, a higher percentage of CD8 + over CD4 + T cells was found in mucocutaneous leishmaniasis (MCL) lesions (Castes & Tapia 1998), compared to localized cutaneous lesions (LCL), although similar distributions of CD4 + and CD8 + in LCL have been reported (Barral et al. 1987, Esterre et al. 1992, Lima et al. 1994. The presence of cytotoxic CD8 + T cells has been reported in peripheral blood of MCL but not in LCL patients (Brodskyn et al. 1997). Expansion of CD8 + T cells occurs in the peripheral blood of individuals vaccinated against leishmaniasis (Mendonça et al. 1995, Gurunathan et al. 2000. Especially, the percentage of activated CD8 + T cells was higher in fast responding than in slow responding volunteers to vaccination (Pompeu et al. 2001).The role of B cells in leishmaniasis is also not clear. High antibody levels are present in the more severe clinical form of the cutaneous disease, namely diffuse cutaneous leishmaniasis (DCL) (Schurr et al. 1986, Mengistu et al. 1990), but B cell depletion does not alter the susceptibility or resistance pattern to Leishmania infection in mice (Babai et al. 1999, Brown & Reiner 1999. It seems that B cells are important to induce anti-Leishmania CD4 + Th1 cells and DTH reaction, in the resistant mouse strain, and take part in the humoral response development in susceptible animals (Scott & Farrell 1982, Scott et al. 1986).Predominance of Th1 cytokines like IFN-γ, IL-12, IL-2 and TNF-α over Th2 cytokines, IL-4, IL-5 IL-10 and TGF-β, is correlated in mice to the resistance profile against Leishmania infection (Belosevic et al. 1989, Chatelain et al. 1992, Lezama-Davila et al. 1992, Barral et al. 1993. Imunological studies in humans demonstrated a combination of Th1 and Th2 cytokines with predominance of Th1 in MCL, Th2 predominate in DCL and predominance of Th1 profile in LCL patients , Castes et...

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Serum antibody specificities to Leishmania aethiopica antigens in patients with localized and diffuse cutaneous leishmaniasis

Cited by 16 publications

References 13 publications

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Identification of a 94‐kilodalton antigen on Leishmania promastigote forms and its specific recognition in human and canine visceral leishmaniasis

Visceral Leishmaniasis in Ververt Monkeys: Immunological Responses During Asymptomatic Infections

B-cell infiltration and frequency of cytokine producing cells differ between localized and disseminated human cutaneous leishmaniases

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