Serum Antibody to Inner Ear Proteins in Patients With Progressive Hearing Loss

Moscicki, Richard A.; Martin, E. San; Quintero, Carlos Hidalgo; Rauch, Steven D.; Nadol, Joseph B.; Bloch, Kurt J.

doi:10.1001/jama.1994.03520080053043

Cited by 159 publications

(48 citation statements)

References 12 publications

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“…Recently, Billings et al [24] reported that the 68-kD band may represent the highly inducible heat shock protein 70 by specific binding to monoclonal antibodies and migration in two-dimensional SDS gel electrophoresis. The report was confirmed subsequently through the protein sequence of this protein by Moscicki et al [22]. The importance of the antibody against this nonspecific stress protein in patients with inner-ear disease, especially PSNHL and Meniere's disease, is not clear at present, and further investigations arc required.…”

Section: Discussionmentioning

confidence: 81%

“…Overall, 32% of their patients with PSNHL demonstrated a positive reaction at 68 kD on Western blot assay using bovine inner ear [24], Subsequently, two different groups confirmed their re sults under similar experimental conditions [22,25], Moreover, Moscicki et al [22] demonstrated a significant relationship between positive blots against a 68-kD pro tein and disease activity or efficacy of steroid therapy on hearing loss. In this study, we found positive blots against a 67-kD protein in 4 of 25 (16.0%) patients with Me niere's disease, 1 of 2 (50.0%) patients with PSNHL, and 1 of 18 (5.6%) patients with other inner-ear diseases, com pared with 0% of controls.…”

Section: Discussionmentioning

confidence: 85%

“…However, in a bovine inner-ear protein study, the antigen specific to the inner ear but nonspecific to disease, described by Gottschlich et al [23] and Billings et al [24], migrated as a doublet at 33-35 kD and thus is unlikely to bear any relationship to the relatively sharp single antigens at 28 or 30 kD that arc reported in this paper. Moscicki et al [22] also reported that reacting protein was rare around 30 kD molecular weight. In a swine inner-ear study, Veldman et al [21] showed patients with inner-ear diseases to have anti bodies against proteins of 27, 45, 50, 68, and 80 kD.…”

Section: Discussionmentioning

confidence: 96%

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Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

Suzuki

Krug²,

Cheng³

et al. 1997

ORL

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Sera from patients with various inner-ear diseases, especially Ménière’s disease, were investigated by Western blot against guinea pig inner-ear proteins. Of 45 patients, 24 (53%) with various inner-ear diseases had antibodies against inner-ear proteins, compared with 0 of 10 (0%) in control subjects without inner-ear diseases. Of the 10 proteins that showed a positive reaction with patient sera, the 28-kD band was unique in that it appeared only in the membranous fraction of the inner ear and was highly positive (28%) in reaction with Ménière’s disease patient sera. The results in the present study with proteins extracted from guinea pig inner ear were consistent with our previous study using proteins from human inner ear, suggesting that the 28-kD protein may be a candidate for detecting autoimmune inner-ear disease.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 96%

See 1 more Smart Citation

Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

Suzuki

Krug²,

Cheng³

et al. 1997

ORL

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“…An imaging study (MRI) was performed to rule out abnormalities of the eighth cranial nerve. The following criteria were applied to support a high suspicion of IMIED [15][16][17] : (1) A positive response was defined as (1) an improvement in pure tone average at 250, 500, 1,000, 2,000 and 4,000 Hz of more than 10 dB and/or (2) the elimination or marked alleviation of vertiginous symptoms.…”

Section: Methodsmentioning

confidence: 99%

Does the Serological Study for Viral Infection in Autoimmune Inner Ear Disease Make Sense?

García-Berrocal

Ramírez‐Camacho

González-García

et al. 2008

ORL

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Viral infections of the labyrinth have been considered a major source of auditory and vestibular system pathology. However, the involvement of virus in the development of immune reactions responsible for immunomediated inner ear disease has not been studied enough. Following viral infection, an effector immune response, humoral (B cell) and/or cytotoxic (T cell) is directed against a virus and it might cross-react with self-protein or autoantigen, evoking an autoimmune response. Since clinically it can be very difficult to establish a viral etiology for such disorders, serologic studies can be used to confirm the suspected diagnosis. Patients affected by immunomediated inner ear disease that had presented an upper respiratory tract infection underwent an immunologic workup study including microbiological study. After the application of this diagnostic protocol, only one patient, that was subsequently diagnosed with Cogan’s syndrome, showed a positive serological test for viral infection. On the basis of the low efficacy of serological testing and due to the lack of evidence, we do not recommend to carry out serologic studies for viral infection.

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“…Progressive SNHL The diagnosis of progressive SNHL was based on criteria reported by Moscicki et al [8] . Thirty-nine patients (16 men and 23 women; mean age 42.2 years; age range 15-69 years) were included.…”

Section: Sudden Snhlmentioning

confidence: 99%

Adverse Effects of Glucocorticoid Therapy for Inner Ear Disorders

García-Berrocal

Ramírez‐Camacho

Lobo

et al. 2008

ORL

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Background: Because of their anti-inflammatory effects and suppression of the immune system, glucocorticoids have been widely used in otolaryngologic disorders and perioperative conditions. Objective: The objective of the present study was to determine the incidence of adverse effects after the administration of glucocorticosteroids in patients affected by diverse inner ear disorders. Methods: One hundred and sixty-three patients affected by sudden sensorineural hearing loss, 39 with progressive sensorineural hearing loss and 16 with fluctuating sensorineural hearing loss were subjected to glucocorticosteroid therapy with 6-methylprednisolone at a starting dose of 1 mg/kg body weight per day; this therapy was tapered during the next 21–28 days. In 20 patients with profound hearing loss (>70 dB), 3 boluses of prednisolone-21-hydrogen-succinate (500 mg per day) were administered. After receiving the boluses, these patients continued with the oral 6-methylprednisolone scheme. Results: Mild adverse effects were observed in 16 patients (7.01%). Only 2 patients (0.9%) with sudden sensorineural hearing loss showed severe complications: peptic ulcer and avascular necrosis of the femoral heads. Conclusions: The low percentage of severe adverse effects observed in the present study validates the use of corticosteroids for the treatment of inner ear disorders although we should not underestimate these rare complications.

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Serum Antibody to Inner Ear Proteins in Patients With Progressive Hearing Loss

Abstract: These results indicate that the presence of circulating antibody to a 68-kd constituent of bovine inner ear extract serves as a marker for IPBSNHL and that its presence correlates with disease activity and responsiveness to corticosteroid treatment.

Cited by 159 publications

References 12 publications

Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

Antibodies against Inner-Ear Proteins in the Sera of Patients with Inner-Ear Diseases

Does the Serological Study for Viral Infection in Autoimmune Inner Ear Disease Make Sense?

Adverse Effects of Glucocorticoid Therapy for Inner Ear Disorders

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