Serum Bile Acids Profiling in Inflammatory Bowel Disease Patients Treated with Anti-TNFs

Roda, Giulia; Porru, Emanuele; Κατσάνος, Κωνσταντίνος; Skamnelos, Alexandros; Kyriakidi, Kallirroi; Fiorino, Gionata; Christodoulou, Dimitrios; Danese, Silvio; Roda, Aldo

doi:10.3390/cells8080817

Cited by 17 publications

(13 citation statements)

References 24 publications

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“…They found significantly increased GCA concentration in the serum of HCC patients. Apart from hepatobiliary diseases, serum BAs were applied to distinguish BA profiles between healthy controls and patients in end‐stage renal disease [89] or inflammatory bowel disease [90,91]. Furthermore, some research groups focused on how serum BA composition is affected by lipid‐rich diet and a peak in total BAs was observed 2 h after intake [79,92].…”

Section: Biological Samplesmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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Section: Biological Samplesmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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“…Furthermore, 13 C-NMR, following incorporation of a labelled precursor, enables the interrogation of cellular pathways such as glycolysis and the tricarboxylic acid (TCA) cycle. 31 P-NMR allows for the detection of metabolites that provide insight into energy or phospholipid metabolism [61,63]. Unlike MS, identification of metabolites by NMR can be performed without prior separation of compounds in the sample.…”

Section: Methodology To Study the Metabolome Of Biological Samplesmentioning

confidence: 99%

“…Examples of commonly used multivariate discriminant tests in metabolomic studies are partial least-squares discriminant analysis (PLS-DA) [24,33,34,41,46,52,53] and principal component analysis (PCA) [13,16,31,34,46,52]. PLS-DA, a multivariate dimensionalityreduction tool, is a supervised classification method, in which the samples are designated into their classes for comparison.…”

Section: Methodology To Study the Metabolome Of Biological Samplesmentioning

confidence: 99%

Metabolomics Insights into Inflammatory Bowel Disease: A Comprehensive Review

2021

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Inflammatory bowel disease (IBD) is a chronic, complex relapsing disorder characterised by immune dysregulation, gut microbiota alteration, and disturbed intestinal permeability. The diagnosis and the management of IBD are challenging due to the recurrent nature and complex evolution of the disease. Furthermore, the molecular mechanism underlying the aetiology and pathogenesis of IBD is still poorly understood. There is an unmet need for novel, reliable, and noninvasive tools for diagnosing and monitoring IBD. In addition, metabolomic profiles may provide a priori determination of optimal therapeutics and reveal novel targets for therapies. This review tries to gather scientific evidence to summarise the emerging contribution of metabolomics to elucidate the mechanisms underlying IBD and changes associated with disease phenotype and therapies, as well as to identify biomarkers with metabolic imbalance in those patients. Metabolite changes during health and disease could provide insights into the disease pathogenesis and the discovery of novel indicators for the diagnosis and prognosis assessment of IBD. Metabolomic studies in IBD have shown changes in tricarboxylic acid cycle intermediates, amino-acid and fatty-acid metabolism, and oxidative pathways. Metabolomics has made progress towards identifying metabolic alterations that may provide clinically useful biomarkers and a deeper understanding of the disease. However, at present, there is insufficient evidence evaluating the predictive accuracy of these molecular signatures and their diagnostic ability, which is necessary before metabolomic data can be translated into clinical practice.

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“…Even a slight difference in BA hydroxylation patterns may exert drastically different biological responses (Li and Chiang 2009;Song et al, 2015). Therefore, BA pro le assessments are garnering increasing interest to screen potential biomarkers for the early diagnosis of human liver and intestinal diseases (Fu et al 2020;Slopianka et al 2017), malnutrition (Lin et al 2020), and healing processes (Roda et al 2019). It was noticed that the BA pro les of sh could be in uenced by diet (Zhang et al 2017), however, it was unclear about the agent in diet that exerted the effect.…”

Section: Introductionmentioning

confidence: 99%

Dietary pectin caused greatly changes in bile acid profiles of Pelteobagrus fulvidraco

Cao¹,

Ren²,

Ni³

et al. 2021

Preprint

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To reveal the impact of dietary fiber (DF) on the bile acid (BA) profiles of fish, yellow catfish (Pelteobagrus fulvidraco) were fed diet containing 300 g kg-1 dextrin (CON diet, control) or pectin (a type of soluble DF, PEC diet) for 7 days, and then the BA profiles were analyzed by UHPLC-MS/MS. A total of 26 individuals of BAs were detected in the fish body, with 8, 10, 14, and 22 individuals of BAs were detected in the liver, serum, bile and hindgut digesta, respectively. The conjugated BAs (CBAs) of fish were dominated by taurine-CBAs (TCBAs). The concentrations of free BAs (FBAs) and the values of FBAs/CBAs in the bile of fish fed the PEC diet were nearly 5 and 7 times higher, respectively, than those in fish fed the CON diet. The values of glycine-CBAs/TCBAs in the liver, serum and bile of fish fed the PEC diet were significantly lower, and in the hindgut digesta was higher than that of fish fed the CON diet (P < 0.05). These results suggested that dietary pectin greatly changed the BA profiles of Pelteobagrus fulvidraco, attributed to inhibition of reabsorption of BAs. Therefore, attention should be paid to the impact to BA homeostasis when replacing fishmeal with DF-rich plant ingredients in the fish diet.

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Serum Bile Acids Profiling in Inflammatory Bowel Disease Patients Treated with Anti-TNFs

Cited by 17 publications

References 24 publications

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Metabolomics Insights into Inflammatory Bowel Disease: A Comprehensive Review

Dietary pectin caused greatly changes in bile acid profiles of Pelteobagrus fulvidraco

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