Serum Biochemical, Histopathology and SEM Analyses of the Effects of the Indian Traditional Herb Wattakaka Volubilis Leaf Extract on Wistar Male Rats

Abstract

Objectives:The present study investigated the protective effect of Wattakaka (W.) volubilis leaf extract against streptozotocin (STZ)-induced diabetes in rats.Methods:Male Wistar rats were divided into five groups (with six rats in each group) and were fed ad libitum. The rats were fasted for sixteen hours before diabetes was induced by injecting a single dose of 90 mg/kg body weight of STZ in 0.9-percent normal saline through an intraperitoneal route. The five groups were as follows: Group 1: normal control (saline-treated), Group 2: untreated diabetic rats, Groups 3 and 4: diabetic rats treated orally with petroleum ether cold maceration extract (PEME) of W. volubilis(50 and 100 mg/kg body weight), and Group 5: diabetic rats treated orally with metformin (250 mg/kg body weight). All rats received treatment for 21 days. For the STZ-induced diabetic rats, the blood-glucose, α-amylase, total protein and alanine transaminase (ALT) levels were measured on days 7,14 and 21 of the treatment with PEME of W. volubilis and the treatment with metformin. Histopathological changes in the liver were examined with hematoxylin-eosin staining. Morphological changes in the liver were also examined with glutaraldehyde fixation.Results:The treatments with PEME of W. volubilis and with metformin in experimental rats by oral injections for 21 days produced reductions in the levels of serum biochemical markers. Histopathology and scanning electron microscopy results showed that the administrations of PEME of W. volubilis and of metformin suppressed the generation of abnormal liver cells in the STZ-treated rats.Conclusion:These results suggest that both PEME of W. volubilis and metformin have a protective effect against STZ-induced diabetes.

Xylitol improves antioxidant, purinergic and cholinergic dysfunction, and lipid metabolic homeostasis in hepatic injury in type 2 diabetic rats

“…According to the findings of the present study, they are similar to those of Gopal et al. (2014) and Lucchesi et al. (2015) who showed destruction of hepatocytes, a progressive loss of organ structure, and dilated sinusoids (Gopal et al., 2014; Lucchesi et al., 2015).…”

“…The liver section revealed loss of cellular mass, dilated sinusoids, deteriorated hepatocytes, and cytoplasmic vacuolations. According to the findings of the present study, they are similar to those of Gopal et al (2014) and Lucchesi et al (2015) who showed destruction of hepatocytes, a progressive loss of organ structure, and dilated sinusoids (Gopal et al, 2014;Lucchesi et al, 2015). The improved liver histologic organization in the animals treated with xylitol indicates their potential therapeutic effect against hepatic dysfunction.…”

“…(2014) and Lucchesi et al. (2015) who showed destruction of hepatocytes, a progressive loss of organ structure, and dilated sinusoids (Gopal et al., 2014; Lucchesi et al., 2015). The improved liver histologic organization in the animals treated with xylitol indicates their potential therapeutic effect against hepatic dysfunction.…”

Yüksek Yağli Di̇yet / Streptozotosi̇n İle İndüklenen Di̇yabeti̇k Farelerde Metformi̇ni̇n Hepatorenal Hasara Karşi Koruyucu Etki̇leri̇

“…Nabi ve ark. (28) Yapılan bir çalışmada STZ ile indüklenmiş diyabetik ratlarda, diyabete bağlı gelişen anormal karaciğer hücrelerinin oluşumunun metformin tedavisi ile baskılandığını bildirmişlerdir (30).…”

Metabolome modulatory effects of Kigelia africana (Lam.) Benth. fruit extracts on oxidative stress, hyperlipidaemic biomarkers in STZ-induced diabetic rats and antidiabetic effects in 3T3 L1 adipocytes

“…Medicinal plants that can effectively combat the menace of DM are expected to have secondary metabolites or phytochemicals ladened with high antioxidant activities. [26] From over 400 tradomedicinal plants that have been reported for the treatment of DM, [27,28] this study screened the hexane fraction of Kigelia africana (Lam.) Benth.…”