Serum biochemical profile in buffalo endometritis and impact of treatment with PGF2α and intrauterine gentamicin infusion on postpartum reproductive performance

Elmetwally, Mohammed A; Elshopakey, Gehad E.; Eldesouky, Ashraf; Eldomany, Wael; Bazer, Fuller W.

doi:10.1007/s11250-020-02406-3

Cited by 5 publications

(3 citation statements)

References 72 publications

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“…The rate of uterine infection in buffaloes is more than in cows (Azawi, 2010). Endometritis have been reported previously in buffaloes (Dawar et al, 2017;Babji et al, 2020;Elmetwally et al, 2020, Hamouda et al, 2020. Postpartum metritis is a major disorders in buffaloes leading to a fetid vulvar discharge and uterine infections can be life-threatening, leading to prolonged inter calving intervals and involuntary culling (Azawi et al, 2013).…”

Section: Introductionmentioning

confidence: 95%

Infertility in Female Buffaloes Due to Some Uterine Disorders

Hamouda,

Al-Jazzar,,

Al-Hizab

et al. 2024

AAVS

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The purpose of this study is to establish that certain uterine disorders may play a significant role in female buffalo infertility. A total of 340 reproductive tracts of female buffaloes have a previous history of infertility was collected from different farms in Egypt through the period of two years. Uteri have been visually and manually inspected as well as examined bacteriologically and histologically. Several uterine lesions either external or internal were noted in 78/340 animals (22.94%) as follows: internal lesions as endometritis in 33 animals (9.71%), metritis in 11 animals (3.24%), endometrial hyperplasia in 7 animals (2.06%), adenomyosis in 5 animals (1.47%) and fibroma in 3 animals (0.88%). External uteri lesions as perimetrial nodules in 10 animals (2.94 %). Perimetrial cysts in 9 animals (2.65%). Furthermore, many bacteria were isolated and the most common was Klebsiella oxytoca, Proteus vulgaris, Proteus vulgaris and Enterobacter aerogenes. The conclusion is that uterine disorders play a crucial role in female buffaloes' infertility. It is highly recommended to use specific clinical parameters and biopsy techniques for early diagnosis in order to proceed with the appropriate treatment.

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Section: Introductionmentioning

confidence: 95%

Infertility in Female Buffaloes Due to Some Uterine Disorders

Hamouda,

Al-Jazzar,,

Al-Hizab

et al. 2024

AAVS

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“…The rst sample was collected in a plain tube without anticoagulant for immediate centrifugation at 3000 rpm for 15 minutes to separate serum, which was stored at -20°C for further biochemical analysis. In the meantime, the second blood sample was collected into a tube containing ethylenediaminetetraacetic acid (EDTA) as an anticoagulant and stored at -80°C for analysis of angiogenic protein translation according to (Elmetwally et al, 2018(Elmetwally et al, , 2020(Elmetwally et al, , and., 2022 Quantitative Real-time Pcr…”

Section: Blood Samplingmentioning

confidence: 99%

Predictive biochemical and oxidative markers for dairy cows with and without retained fetal placenta

Elmetwally¹,

Shalapy

Montaser

2022

Preprint

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The changes in the expression profile of the angiogenicgenes and serum biomarkers were investigated in the cows with the retained placenta after normal parturition. Retained fetal membranes (RFM) are considered one of the main reproductive disorders in dairy cattle. Highlight the effects of Excede® (Ceftiofur Crystalline Free Acid: Zoetis, USA) in combination with Oxytocin (Oxytocin®, each ml contains 10 IU oxytocin, ADWIA, Egypt), Estrumate® (250µg cloprostenol: synthetic analogue of PGF2ά) and Flunixin Meglumine® (nonsteroidal anti-inflammatory drug) on the reproductive performance in dairy cows. A total of 16 cows were allocated into two groups: group 1 (n = 8) the cows that have a normal loosed placenta, and group 2 (n = 8). The cows that have retained fetal membranes for more than 24 h after parturition. Blood with EDTA was collected from the tail vein for studying the expression of angiogenic proteins including vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor1 (VEGFR1), and RANTES genes. Serum was collected at a one-week interval before/after parturition for the study of biochemical changes. The VEGF expression decreased ten folds in cows with retained than normally dropped placentae (p ˂ 0.0001). The vascular endothelial growth factor receptor2 (VEGFR2) showed a threefold increase in cows with normal loosed than retained placentae (p˂0.01). The RANTES was increased in cows with retained rather than normal placentae by about 7 folds (p˂0.01). Phosphorus and total proteins were significantly lower (p˂0.05) in RFM cows one week after parturition. The serum albumin, TG, HDL, and cholesterol were significantly lower (p˂0.05) in RFM before, at, and after parturition. CPK was significantly higher in RFM before, at, and after parturition (p˂0.05). Increased values of MDA and decreased values of CAT, SOD, and R-GSH were detected in the blood of cows suffering from RFM (p˂0.05). Reproductive performance parameters for all studied cows were days to first estrous, number of service per conception and pregnancy rate. All reproductive parameters demonstrated significant (P < 0.05) changes between treated animals. The translation of VEGF and its receptor and RANTESmRNA at the time of parturition in dairy cows could be proxy biomarkers for the prediction of retained fetal membranes (RFM). In the same line, the activity of total proteins, serum albumin, TG, HDL, cholesterol, and CPK would be useful in the prediction of the RFM in dairy cows. Altogether, the obtained results indicated that retained fetal membranes in cows could be treated successfully without any interference by systemic injection of cephalosporin with caring of temperature. The reproductive performance criteria for all cows studied were the time of first estrus, the number of services per conception, and pregnancy rates. All parameters of reproductive performance showed significant changes (P < 0.05) among the treated animals. The results also indicated that cephalosporin can be used by systemic injection to treat placental retention with caring of the animal's temperature.

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“…Numerous studies on the use of B mode ultrasound in postpartum buffaloes have been published. [14][15][16]. Doppler ultrasonography is a non-invasive method that may be utilized both throughout gestation and after giving birth [5,12,17].…”

Section: Introductionmentioning

confidence: 99%

Application of Color Doppler Ultrasound in Egyptian Buffalo Reproduction

Elmetwally

Gohar

Tawfik³

et al. 2023

Egyptian Journal of Veterinary Sciences

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T HE Color Doppler ultrasonography is widely utilized in equines, dairy cattle, and small ruminants as a safe, non-invasive means of monitoring reproductive performance. Non-intrusive color Doppler ultrasonography was utilized to evaluate high-risk pregnancies and the health of the fetus. The alterations that take place in farm animals after giving birth are thought to be crucial in predicting future fertility. B mode ultrasonography has been applied in several studies on postpartum buffaloes. Color Doppler ultrasonography is a non-invasive technique that can be used before, during, and after delivery. Using the uterine and umbilical arteries, it is possible to evaluate the perfusion of the uteroplacental and fetoplacental blood flow in buffaloes. The motion of the transmitter and receiver affects the frequency of an ultrasonic pulse, which is a phenomenon called the Doppler Effect, and was initially described by Christian Doppler. Nowadays, the use of color Doppler ultrasonography has followed the same pattern as that of cows and other animals. Doppler ultrasound application's basis is likened to cows. The blood supply to the uterus is remarkably similar. Recent research have described the features of vaginal blood circulation in buffaloes throughout gestation and the early puerperium in addition to the uterine blood flow. The impact of vaginal blood circulation on buffalo fertility needs to be studied in the future. We highlight the different benefits of non-invasive color Doppler ultrasound applications in the buffalo's reproduction.

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Serum biochemical profile in buffalo endometritis and impact of treatment with PGF2α and intrauterine gentamicin infusion on postpartum reproductive performance

Cited by 5 publications

References 72 publications

Infertility in Female Buffaloes Due to Some Uterine Disorders

Infertility in Female Buffaloes Due to Some Uterine Disorders

Predictive biochemical and oxidative markers for dairy cows with and without retained fetal placenta

Application of Color Doppler Ultrasound in Egyptian Buffalo Reproduction

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