Serum biochemical values in fallow deer ( Dama dama L.) from different habitats in Croatia

Poljičak-Milas, Nina; Slavica, Alen; Janicki, Zdravko; Robić, Mirna; Belić, Maja; Milinković-Tur, Suzana

doi:10.1007/s10344-003-0003-2

Cited by 16 publications

(24 citation statements)

References 19 publications

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“…The average concentration of potassium was 8.12 mmol/L. These values are similar to those reported by Onderscheka et al [29] Mean glucose concentration was 6.56 mmol/L which is lower than those decribed by Slamecka et al [30] (7.15 mmol/L) and higher than those by Onderscheka et al [29] Nimitsuntiwong et al [31] and Policjak-Milas et al [32] found higher blood concentration (3.23 mmol/L) of glucose in wild animals than domestic animals. This may be associated with higher metabolic rate and stress.…”

Section: Downloaded By [Rmit University] At 22:42 16 September 2013supporting

confidence: 89%

Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters

Kolesárová

Slamečka²,

Jurčík³

et al. 2008

Journal of Environmental Science and Health, Part A

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The purpose of this study was to examine concentrations of selected heavy metals in the liver and kidney of brown hares (Lepus europaeus). In addition, correlations between heavy metals and biochemical parameters in blood plasma were determined. The average concentrations of heavy metals (mmol/L) +/- SD were as follows: liver: Pb 0.221 +/- 0.189, Cd 0.160 +/- 0.140, Hg 0.021 +/- 0.030, kidney: Pb 0.115 +/- 0.125, Cd 1.570 +/- 1.103, Hg 0.030 +/- 0.053. The average concentrations of biochemical parameters in the blood plasma were as follows: Ca 3.16 mmol/L, P 2.19 mmol/L, Mg 1.40 mmol/L, Na 148.71 mmol/L, K 8.12 mmol/L, glucose 6.56 mmol/L, total proteins 56.49 g/L, urea 5.00 mmol/L, total lipids 1.40 g/L, bilirubin 3.97 micro mol/L, cholesterol 1.53 mmol/L, aspartate aminotransferase (AST) 6.06 micro kat/L and alanine aminotransferase (ALT) 1.94 micro kat/L. Average levels of hormones (ng/mL) were as follows: testosterone 2.94, androstendiol 0.13, estradiol 501.59, progesterone 6.63, oxytocin 328.60. Tissue analysis showed an accumulation of lead, cadmium and mercury in the liver and kidney of brown hares. There were no significant correlations between levels of heavy metals in liver, kidney, and biochemical parameters.

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Section: Downloaded By [Rmit University] At 22:42 16 September 2013supporting

confidence: 89%

Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters

Kolesárová

Slamečka²,

Jurčík³

et al. 2008

Journal of Environmental Science and Health, Part A

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“…From live animals, blood was drawn by vacuum collection tube ("Becton-Dickinson," Meyland Cedex, France) using the technique described by Poljičak-Milas et al (2004), whereas from shot animals, blood was taken directly from the heart using a long syringe, as modification of the Sebek method, which was described by Cvetnić et al (2003). Blood samples were transported to the laboratory within 12 h, and all sera were tested by microscopic agglutination test (MAT) for ten leptospiral antigens (serovars: Icterohaemorrhagiae, Australis, Pomona, Grippotyphosa, Sejroe, Bataviae, Hardjo, Ballum, Saxkoebing, and Tarassovi).…”

Section: Serological Testsmentioning

confidence: 99%

Incidence of leptospiral antibodies in different game species over a 10-year period (1996–2005) in Croatia

Slavica

Cvetnić

Milas³

et al. 2007

Eur J Wildl Res

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During the 10-year survey (1996)(1997)(1998)(1999)(2000)(2001)(2002)(2003)(2004)(2005), a total of 868 blood samples from different game species in Croatia were analyzed for the presence of leptospiral antibodies. The specific antibodies (AB) were detected in 242 samples (27.88%). According to the species in red deer (Cervus elaphus), the antibodies against six different leptospiral serovars were found in 43 of 226 analyzed sera (19.02%). The most frequent antigen serovars in the deer population were Pomona and Ballum (with the same frequency of 23.6%), whereas the highest titer was recorded for serovar Sejroe (1:800). In the analyzed roe deer (Capreolus capreolus) serum samples, a low level of leptospiral antibodies (6.07%) was determined, with just two AB for antigen serovars-Australis and Sejroe. In wild boar (Sus scrofa), leptospiral antibodies were detected in 151 of 431 samples analyzed (35.03%), with AB for nine antigen serovars. The serovars most frequently found were Australis (48.70%) and Pomona (22.70%), and these serovars also recorded the highest titer (1:3,200). Among brown bear (Ursus arctos) samples, leptospiral antibodies were detected in 25.00% of the samples, with four AB for antigen serovars, of which the most frequent was Icterohaemorrhagiae (>40%). This serovar had the highest recorded titer (1:400). From 112 analyzed red fox (Vulpes vulpes) samples, leptospiral antibodies were found in 35 samples (31.25%). The determined antibodies were specific for four antigen serovars, of which the most frequent (46.2%) and with highest titer (1:1600) was serovar Australis. No antibodies (28/0) were recorded in mouflon (Ovis musimon). The most important game species from an epizootiological point of view in the studied area were certainly wild boar and red foxes. With strong serological reactions, these two species could be emphasized as important hosts for Leptospira interrogans sv. Australis in Croatia, but for their declaration as 'maintaining hosts,' isolation of sv. Australis is needed. According to aerial distribution, the highest number of positive samples from different game species was recorded in the central and eastern parts of Croatia, known as the 'historical natural foci' of leptospirosis-the regions of Posavina, Podravina, Slavonija, and Baranja. In contrast, the areas of Kordun and Gorski Kotar are declared as leptospira low-risk regions for the game species studied.

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“…The collection of blood samples was performed aseptically, directly from the heart of shot foxes using a long needle (Poljicak-Milas et al, 2004) using a modification of the method described by Slavica et al (2000). Blood samples were stored in serum separator tubes, centrifuged at 2200 rpm and transported to a laboratory within 12 hours.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of leptospiral antibodies in the red fox (Vulpes vulpes) population of Croatia

Slavica¹,

Dežđek²,

Konjević³

et al. 2011

Vet. Med.

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Over a period of five years (2005)(2006)(2007)(2008)(2009)(2010) a total of 358 red fox (Vulpes vulpes) serum samples were collected during regular hunting procedures, mostly in the continental part of Croatia. All samples were tested using the microscopic agglutination test (MAT) for the presence of specific antibodies (Ab) to 12 Leptospira spp. pathogenic serovars. Specific antibodies for 11 different Leptospira spp. serovars (Australis, Sejroe, Icterohaemorrhagiae, Saxkoebing, Grippotyphosa, Tarassovi, Ballum, Pomona, Poi, Batavie, and Canicola) were detected in 121 red fox serum samples (33.8%). Among the positive samples antibodies for serovar Australis were found most frequently (32.1%), followed by Abs for serovar Sejroe (18.2%) and Icterohaemorrhagiae (13.2%). The highest Ab titre (1 : 3200) was recorded for serovar Australis and together with serovars Sejroe and Icterohaemorrhagiae this serovar showed a statistically significant frequency in cross-reactions (recorded in 23.1% cases). High Ab titres for serovars Grippotyphosa and Tarassovi (1 : 800) were detected for the first time in red foxes from the continental part of Croatia. The significant percentage (≈ 35%) of seropositive reactions to Leptospira spp. serovars implies the presence of pathogenic agents in the natural habitats of red foxes in Croatian low-land territories. The high Ab prevalence for specific serovars (Australis and Sejroe) detected in the fox population suggests predator-prey chain transmission of leptospirosis between red foxes and small terrestrial mammals, whilst the high Ab prevalence for serovar Icterohaemorrhagiae indicates an occasional contact of red foxes with rats. Red foxes can be thought of as susceptible hosts for serovars Australis and Sejroe and a possible link in leptospirosis transmission to other wild carnivores such as martens, weasels and ferrets. In contrast, this serologic survey revealed no evidence of serovar Canicola interchange between domestic carnivores (dogs) and red foxes. It can be concluded that red foxes are reliable sentinels for the epidemiological monitoring of leptospirosis, especially in lowland habitats.Keywords: red fox; Leptospira spp.; serology; Croatia List of abbrevations Ab = antibodies; F = female; M = male; MAT = microscopic agglutination test

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Serum biochemical values in fallow deer ( Dama dama L.) from different habitats in Croatia

Cited by 16 publications

References 19 publications

Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters

Environmental levels of cadmium, lead and mercury in brown hares and their relation to blood metabolic parameters

Incidence of leptospiral antibodies in different game species over a 10-year period (1996–2005) in Croatia

Prevalence of leptospiral antibodies in the red fox (Vulpes vulpes) population of Croatia

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