Serum biomarkers in idiopathic pulmonary fibrosis

Blink, Bernt van den; Wijsenbeek, Marlies; Hoogsteden, Henk C.

doi:10.1016/j.pupt.2010.08.001

Cited by 44 publications

(14 citation statements)

References 80 publications

(84 reference statements)

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“…This should be taken with precaution because hemoglobin is generally regarded as a marker for lysis of erythrocytes during sample draw [49]. Intriguingly, most of previously reported IPF biomarkers [7–16, 28–42, 50–70] were not identified in our study. Therefore, the present findings are promising for the discovery and development of novel biomarkers for IPF.…”

Section: Discussioncontrasting

confidence: 53%

“…For instance, high levels of matrix metalloproteinase-8 (MMP-8), pepsin, BALF YKL-40, S100A9 and MRP14 have been found elevated in the BALFs of IPF patients [15, 28–31]. Similarly, elevated serum levels of vascular endothelial growth factor (VEGF), MMP3, CXCL13, heat shock protein 47 (HSP47) and several other proteins were equally reported in IPF [7, 13, 15, 30, 32–42]. In the present study, iTRAQ labeling coupled with 2D LC-MS/MS analysis was used to identify differentially expressed proteins in serum of IPF patients.…”

Section: Discussionmentioning

confidence: 99%

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iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

et al. 2017

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Idiopathic pulmonary fibrosis (IPF) is a gradual lung disease with a survival of less than 5 years post-diagnosis for most patients. Poor molecular description of IPF has led to unsatisfactory interpretation of the pathogenesis of this disease, resulting in the lack of successful treatments. The objective of this study was to discover novel noninvasive biomarkers for the diagnosis of IPF. We employed a coupled isobaric tag for relative and absolute quantitation (iTRAQ)-liquid chromatography–tandem mass spectrometry (LC–MS/MS) approach to examine protein expression in patients with IPF. A total of 97 differentially expressed proteins (38 upregulated proteins and 59 downregulated proteins) were identified in the serum of IPF patients. Using String software, a regulatory network containing 87 nodes and 244 edges was built, and the functional enrichment showed that differentially expressed proteins were predominantly involved in protein activation cascade, regulation of response to wounding and extracellular components. A set of three most significantly upregulated proteins (HBB, CRP and SERPINA1) and four most significantly downregulated proteins (APOA2, AHSG, KNG1 and AMBP) were selected for validation in an independent cohort of IPF and other lung diseases using ELISA test. The results confirmed the iTRAQ profiling results and AHSG, AMBP, CRP and KNG1 were found as specific IPF biomarkers. ROC analysis indicated the diagnosis potential of the validated biomarkers. The findings of this study will contribute in understanding the pathogenesis of IPF and facilitate the development of therapeutic targets.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

et al. 2017

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“…Our studies suggest that the MARGS classifier is a valid candidate for a crossplatform, cross-organ classifier of normal tissue vs. fibrotic conditions of different etiologies. Obvious next steps are the application of the classifier to blood sample gene expression data, to see whether a minimally invasive, easily applicable gene expression marker could accompany or outperform other minimally and non-invasive methods [31][32][33][34]. Given that, e.g., plasma levels of TIMP1 protein have been found elevated in diastolic dysfunction [35], transfer of our data to, for instance, peripheral blood appears promising.…”

Section: Discussionmentioning

confidence: 99%

A subset of metzincins and related genes constitutes a marker of human solid organ fibrosis

Rödder

Scherer²,

Körner

et al. 2011

Virchows Arch

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Metzincins and functionally related genes play important roles in extracellular matrix remodeling both in healthy and fibrotic conditions. We recently presented a transcriptomic classifier consisting of 19 metzincins and related genes (MARGS) discriminating biopsies from renal transplant patients with or without interstitial fibrosis/tubular atrophy (IF/TA) by virtue of gene expression measurement (Roedder et al., Am J Transplant 9:517-526, 2009). Here we demonstrate that the same algorithm has diagnostic value in non-transplant solid organ fibrosis. We used publically available microarray datasets of 325 human heart, liver, lung, kidney cortex, and pancreas microarray samples (265 with fibrosis, 60 healthy controls). Expression of nine commonly differentially expressed genes was confirmed by TaqMan low-density arrays (Applied Biosystems, USA) in 50 independent archival tissue specimens with matched histological diagnoses to microarray patients. In separate and in combined, integrated microarray data analyses of five datasets with 325 samples, the previously published MARGS classifier for renal post-transplant IF/TA had a mean AUC of 87% and 82%, respectively. These data demonstrate that the MARGS gene panel classifier not only discriminates IF/TA from normal renal transplant tissue, but also classifies solid organ fibrotic conditions of human pancreas, liver, heart, kidney, and lung tissue samples with high specificity and accuracy, suggesting that the MARGS classifier is a cross-platform, cross-organ classifier of fibrotic conditions of different etiologies when compared to normal tissue.

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“…El papel en el diagnóstico de determinados biomarcadores como KL-6, SP-A y SP-D, fibrocitos circulantes y metaloproteinasas 1 y 7 están bajo investigación en el momento actual. Ninguno de ellos de manera individual ha sido capaz de predecir con seguridad la presencia o ausencia de FPI, por lo que es posible que un panel compuesto de distintos biomarcadores pueda emplearse en el futuro con utilidad diagnóstica 26 .…”

Section: Características Clínicas Y Pruebas Complementariasunclassified

Una mirada general a las enfermedades pulmonares intersticiales y una específica a la fibrosis pulmonar idiopática

Rodríguez¹,

Montero-Martínez²,

González³

2013

Gal. Clin.

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Serum biomarkers in idiopathic pulmonary fibrosis

Cited by 44 publications

References 80 publications

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

A subset of metzincins and related genes constitutes a marker of human solid organ fibrosis

Una mirada general a las enfermedades pulmonares intersticiales y una específica a la fibrosis pulmonar idiopática

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