Serum butyrylcholinesterase in type 2 diabetes mellitus: a biochemical and bioinformatics approach

Abstract

Background: Butyrylcholinesterase is an enzyme that may serve as a marker of metabolic syndrome. We (a) measured its level in persons with diabetes mellitus, (b) constructed a family tree of the enzyme using nucleotide sequences downloaded from NCBI. Butyrylcholinesterase was estimated colorimetrically using a commercially available kit (Randox Lab, UK). Phylogenetic trees were constructed by distance method (Fitch and Margoliash method) and by maximum parsimony method.Results: There was a negative correlation between serum total cholesterol and butyrylcholinesterase (-0.407; p < 0.05) and between serum LDL cholesterol and butyrylcholinesterase (-0.435; p < 0.05). There was no statistically significant correlation among the other biochemical parameters. In the evolutionary tree construction both methods gave similar trees, except for an inversion in the position of Sus scrofa (M62778) and Oryctolagus cuniculus (M62779) between Fitch and Margoliash, and maximum parsimony methods. Conclusion:The level of butyrylcholinesterase enzyme was inversely related to serum cholesterol; dendrogram showed that the structures from evolutionarily close species were placed near each other.

Resveratrol Derived Butyrylcholinesterase Inhibitors

“…ACE from the electric eel and BCE from equine serum were used as enzymes. The enzyme BCE from equine serum shows high homology with human BCE; it was estimated that the rate of evolution for this enzyme was about 2.2 million years for 1% amino acid change . The ACE from the electric eel shows high homology with human nerve system ACE subtype.…”

Status of Acetylcholinesterase and Butyrylcholinesterase in Alzheimer&#39;s Disease and Type 2 Diabetes Mellitus

“…Studies have shown that BuChE may be indirectly contributing to the pathogenesis of T2DM by causing insulin resistance [36]. In fact, a locus on chromosome 3q27, which is near the chromosomal locus of BuChE (3q26), has been linked to T2DM in a French population in one study [37].…”

Serum levels of acetylcholinesterase in metabolic syndrome

“…In the same vein, in addition to its role in cholinergic neurotransmission, acetylcholine exerts a potential anti-in ammatory e ect mediated by "cholinergic anti-in ammatory re ex" (5). Accordingly, a positive correlation of the blood serum level of acetylcholinesterase, butyrylcholinesterase and C-reactive protein (CRP) was reported in obese and diabetic patients (6)(7)(8)(9)(10).…”