Background: It is already well known the importance of hormones such as progesterone, cortisol and melatonin on the reproduction of females of various species. However, as regards the goat species, there is still a lack of data, especially in animals used in oocyte donation programs for subsequent in vitro production of embryos. The objective of this study was to verify the profile of progesterone, cortisol and melatonin during the hormonal treatment of ovarian stimulation in the anestrous, transition and reproductive season in Alpine goats using artificial and natural photoperiod. Seventeen female Alpine goats were reared at latitude 21°15'22''S. The animals were treated with 10mg oFSH (Ovagen, ICP, New Zealand) in decreasing doses for four consecutive days at the end of a progestin treatment with CIDR® (Intervet, New Zealand) at the reproductive season (G1,n=7) and anestrous with artificial photoperiod (G2,n=5) or natural (G3,n=5). Blood samples were taken before, during and after the period of ovarian stimulation and during oocyte harvest by laparotomy. Blood collection for melatonin was performed overnight, for progesterone and cortisols were performed for 16days in the morning. The values were presented as mean, standard deviation and median for the groups and compared by non-parametric analysis (Kruskal-Wallis, analysis of variance and Friedman). With regard to progesterone, there was a difference (P<0.05) between the three groups studied and in the two periods (reproductive season/anestrous), with higher values in the reproductive season. Statistical differences (P>0.05) were not observed for cortisol between groups, however, after laparotomy there was a significant increase (P<0.05) in the operated goats (G1:40.94±18,53nmol/L) compared to those not operated at the reproductive season (G2:17.86±14.90nmol/L and G3:16.00±16.51nmol/L). Serum cortisol values were lower than those of the reproductive season for all groups, G1(13.18±9.43nmol/L, natural photoperiod, non-laparotomized goats), G2(26,70±15,39nmol/L, artificial photoperiod, laparotomized goats) and G3 (35.35±17.10nmol/L, natural photoperiod, laparotomized goats). However, the surgical group (G2 in artificial photoperiod) presented serum cortisol levels between the nonsurgical group (G1 in the natural photoperiod) and the surgical group (G3 in the natural photoperiod), leading to the belief that melatonin might have interfered with the values Cortisol of these animals in anestrus. During the experimental period, melatonin values were always high in all groups in the anestrous period (G1:68.19pg/mL, G2: 45.53pg/mL and G3:58.28pg/mL). Conclusion: Under the conditions of this study, it is possible to verify an influence of the season (reproductive or anestrous), and possibly melatonin, on the secretion of cortisol in these animals.