Serum cytokine profiles of patients with interstitial lung disease associated with anti-CADM-140/MDA5 antibody positive amyopathic dermatomyositis

Abstract

These results confirmed that anti-CADM-140/MDA5 antibody levels could predict outcomes of ADM-ILD. Relationship analyses suggested that CX3CL1 might be involved in the pathogenesis of anti-CADM-140/MDA5 antibody positive ADM-ILD.

Multicenter Prospective Study of the Efficacy and Safety of Combined Immunosuppressive Therapy With High‐Dose Glucocorticoid, Tacrolimus, and Cyclophosphamide in Interstitial Lung Diseases Accompanied by Anti–Melanoma Differentiation–Associated Gene 5–Positive Dermatomyositis

“…Dr. Hirata has received consulting fees, speaking fees, and/or honoraria from AbbVie, Asahi Kasei Pharma, Asahi Kasei Medical, Astellas Pharma, in the study if they were diagnosed as having definite, probable, or possible DM or CADM with ILD-without immunosuppressive treatment-before admission to one of the registered hospitals. Serum anti-MDA-5 was detected by immunoprecipitation (IP) assay using 35 S-labeled HeLa cells and confirmed by anti-MDA-5 enzyme-linked immunosorbent assay (ELISA; Mesacup). Patients with the following conditions were excluded: active tuberculosis, active hepatitis B virus infection, cancer, and patients <16 years of age.…”

“…Subsequently, the IgG-coated Sepharose was washed, then suspended in 400 ml of IP buffer for polypeptide studies. HeLa cells (2 × 10 7 ) in 100 ml of methionine-free medium were labeled with 18.5 MBq of 35 S-methionine (Perkin Elmer) overnight at 37°C; they were then sonicated in 2 ml of IP buffer, and the supernatant was recovered by centrifugation. Antibody-coated Sepharose beads were mixed with 100 ml of 35 S-methionine-labeled HeLa cell extracts, then rotated at 4°C for 2 hours.…”

“…HeLa cells (2 × 10 7 ) in 100 ml of methionine-free medium were labeled with 18.5 MBq of 35 S-methionine (Perkin Elmer) overnight at 37°C; they were then sonicated in 2 ml of IP buffer, and the supernatant was recovered by centrifugation. Antibody-coated Sepharose beads were mixed with 100 ml of 35 S-methionine-labeled HeLa cell extracts, then rotated at 4°C for 2 hours. Subsequently, Sepharose beads were washed 5 times, then suspended in sodium dodecyl sulfate sample buffer; polypeptides were then fractionated by 6.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and detected by autoradiography.…”

Therapeutic plasma exchange for clinically amyopathic dermatomyositis (CADM) associated with rapidly progressive interstitial pneumonia

“…In addition, we did not evaluate changes to other inflammatory cytokines, including serum interleukin (IL)-6, IL-8, IL-10, IL-18, interferon (IFN)-α, IFN-γ, macrophage colony-stimulating factor, CX3CL1, or messenger RNA levels of CD11b in circulating neutrophils, which have also been indicated to represent pathogenic factors for RPIP. [20][21][22][23][24][25] Activation of monocytes and macrophages through these factors has been suggested to underlie the pathophysiology of anti-MDA5 Ab-positive DM patients. 20,25 Further studies should be conducted to elucidate whether removal of these factors with TPE contributes to disease control.…”

Cytokine profiles of amyopathic dermatomyositis with interstitial lung diseases treated with mycophenolate

“…We analysed the relationship between the titres of anti-MDA-5 autoantibodies and each cytokine/GF protein concentration before immunosuppressive treatment to find the highest Spearman's rank correlation coefficients in CX3CL1 (r = 0.8897, P < 0.001) [4]. Serum cytokine/GF protein in this case demonstrated that the concentrations of FGF-2, IL-1ra, IL-17A, IP-10, and MCP-1 decreased after addition of MMF as well as CX3CL1.…”