Serum decreases the size of Metafectene-and Genejammer-DNA complexes but does not affect significantly their transfection activity in SCCVII murine squamous cell carcinoma cells

Konopka, Krystyna; Overlid, Nathan; Nagaraj, Anitha C.; Düzgüneş, Nejat

doi:10.2478/s11658-006-0015-5

Cited by 21 publications

(20 citation statements)

References 50 publications

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“…S6). This reduction in size was previously reported for colloidal carriers and it was speculated that the change in dispersant viscosity and refractive index could potentially affect the size analysis with DLS [30]. Average droplet size and size distribution of nanoemulsions remained unaltered after incubation at pH 5.0 and 7.4 for 5 days (Fig.…”

Section: Resultssupporting

confidence: 77%

Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model

Patel

Beaino

Anderson

et al. 2015

Clinical Immunology

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Targeting macrophages for therapeutic and diagnostic purposes is an attractive approach applicable to multiple diseases. Here, we present a theranostic nanoemulsion platform for simultaneous delivery of an anti-inflammatory drug (celecoxib) to macrophages and monitoring of macrophage migration patterns by optical imaging, as measurement of changes in inflammation. The anti-inflammatory effect of the theranostic nanoemulsions was evaluated in a mouse inflammation model induced with complete Freund's adjuvant (CFA). Nanoemulsions showed greater accumulation in the inflamed vs. control paw, with histology confirming their specific localization in CD68 positive macrophages expressing cyclooxygenase-2 (COX-2) compared to neutrophils. With a single dose administration of the celecoxib-loaded theranostic, we observed a reduction in fluorescence in the paw with time, corresponding to a reduction in macrophage infiltration. Our data strongly suggest that delivery of select agents to infiltrating macrophages can potentially lead to new treatments of inflammatory diseases where macrophage behavior changes are monitored in vivo.

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Section: Resultssupporting

confidence: 77%

Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model

Patel

Beaino

Anderson

et al. 2015

Clinical Immunology

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“…These results were fully supported by electrophoretic mobility shift assay where TP1, TP2 retarded pDNA at lower concentration as compared to bPEI, while TP3–TP5 required higher amounts of polymers to retard the same amount of pDNA. Further, all the TPs complexes showed negative zeta potential in the presence of serum as reported in the literature [ 30 ].…”

Section: Resultssupporting

confidence: 61%

Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors

Mahato

Yadav

Kumar

et al. 2014

BioMed Research International

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Previously, we demonstrated that 6-(N,N,N′,N′-tetramethylguanidinium chloride)-hexanoyl-polyethylenimine (THP) polymers exhibited significantly enhanced transfection efficiency and cell viability. Here, in the present study, we have synthesized a series of N,N,N′,N′-tetramethylguanidinium-polyethylenimine (TP1-TP5) polymers via a single-step reaction involving peripheral primary amines of bPEI and varying amounts of 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU). These polymers were found to interact efficiently with negatively charged pDNA and formed stable complexes in the size range of ~240–450 nm. Acid-base titration profiles revealed improved buffering capacity of TP polymers as compared to bPEI. Transfection and cytotoxicity assays performed with TP/pDNA complexes on HEK293, CHO, and HeLa cells showed significantly higher transfection efficiency and cell viability with one of the complexes, TP2/pDNA complex, exhibited the highest transfection efficiency (~1.4–2.3-fold) outcompeting native bPEI and the commercially available transfection reagent, Lipofectamine 2000. Compared to previously reported THP polymers, the transfection efficiency of TP/pDNA complexes was found to be lower, as examined by flow cytometry. These results highlight the importance of the hydrophobic C-6 linker in THP polymers in forming compact nanostructures with pDNA, which might lead to efficient uptake and internalization of the complexes; however, the projected TP polymers offer an advantage of their rapid and economical one-step synthesis.

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“…Significantly higher levels of luciferase activity were driven by the CMV promoter than by survivin promoters. SCCVII cells were readily transfectable [53]. The CMV promoter-driven, Metafectene PRO-mediated expression of luciferase in Tab SCCVII cells was very high.…”

Section: Transgene Expression In Murine Cells Driven By the Survivin mentioning

confidence: 99%

“…At first, the transfection efficiency of Metafectene and Metafectene PRO was optimized in HeLa, HSC-3 and H413 cells as described previously [53]. Metafectene and Metafectene PRO were complexed with the pCMV.Luc plasmid at reagent:DNA ratios of 1 µl:0.5 µg, 2 µl:0.5 µg, 2 µl:1 µg or 4 µl:1 µg DNA.…”

Section: Transgene Expression In Human Cells Driven By the Survivin Amentioning

confidence: 99%

Correlation between the levels of survivin and survivin promoter-driven gene expression in cancer and non-cancer cells

Konopka

Spain

Yen

et al. 2009

Cellular and Molecular Biology Letters

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Abbreviations used: DMEM -Dulbecco's modified Eagle's MEM medium; FBS -fetal bovine serum; HNSCC -head and neck squamous cell carcinoma; IAP -inhibitor of apoptosis; OSCC -oral squamous cell carcinoma; RLU -relative light units luciferase driven by the CMV and survivin promoters in murine cells was much higher than that in human cells. The cells displayed very different susceptibilities to transfection; nevertheless, high CMV-driven luciferase activity appeared to correlate with high survivin-promoter driven luciferase expression. The survivin concentration in lysates of cancer cells ranged from 5.8 ± 2.3 to 24.3 ± 2.9 ng/mg protein (mean, 13.7 ng/mg). Surprisingly, elevated survivin protein was determined in lysates of non-tumor-derived cells. Survivin levels for GMSM-K and 184A-1 cells, were 16.7 ± 8.7 and 13.5 ± 6.2 ng/mg protein, respectively. The expression of endogenous survivin did not correlate with the level of survivin promoter-driven transgene activity in the same cells. The expression of survivin by non-tumorigenic, transformed cell lines may be necessary for their proliferative activity. The level of survivin promoter-driven gene expression achieved via liposomal vectors in OSCC cells was too low to be useful in cancer-cell specific gene therapy.

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Serum decreases the size of Metafectene-and Genejammer-DNA complexes but does not affect significantly their transfection activity in SCCVII murine squamous cell carcinoma cells

Cited by 21 publications

References 50 publications

Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model

Theranostic nanoemulsions for macrophage COX-2 inhibition in a murine inflammation model

Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors

Correlation between the levels of survivin and survivin promoter-driven gene expression in cancer and non-cancer cells

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