Serum-derived exosomes induce proinflammatory cytokines production in Cynoglossus semilaevis via miR-133-3p

Sun, Zhanpeng; Liu, Xiaozhu; Lu, Mei-Yi; Zhang, Xiao; Sun, Jinsheng

doi:10.1016/j.dci.2022.104497

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…Furthermore, the presence of EVs was confirmed in the serum of Nile tilapia ( Oreochromis niloticus ), Chinese tongue sole ( Cynoglossus semilaevis ), Grass carp ( Ctenopharyngodon idellus ), Atlantic halibut ( Hippoglossus hippoglossus ), Mandarin fish ( Siniperca chuatsi ), Rohu ( Labeo rohita ) and Crucian carp ( Carassius auratus ) (Zhao et al 2021 ; Sun et al 2022 , 2017 ; Zhu et al 2022 ; Zhang et al 2021 ; He et al 2021 ; Tang et al 2022 ).…”

Section: Discussionmentioning

confidence: 98%

“…Sun et al ( 2022 ) isolated serum exosomes using ExoQuickTM and ultracentrifugation from Chinese tongue sole ( Cynoglossus semilaevis ) and analyzed the miRNA expression and inflammation in EVs after Vibrio harveyi infection. EVs were characterized with a spherical shape with a diameter of 30–295 nm in 3.3E + 9 particles/ml and the canonical EV markers CD63 and CD81 using Western blot.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

Pelyhe,

Sturve

2023

Fish Physiol Biochem

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Extracellular vesicles (EVs) are 50–1,000 nm lipid bilayer-bound vesicles, released into the extracellular environment by various cell types for intercellular communication purposes. The quantitative and qualitative characteristics of EVs can be affected by stress and pathological conditions. The majority of extracellular vesicle (EV) studies have been performed on mammalian cell lines or bodily fluids. EVs have been previously described from bodily fluids like plasma, serum or mucus in different fish species, however the available knowledge of fish cell line derived EVs is limited and in the vast majority of studies, the overall focus is on small EVs (< 200 nm). We isolated large and small extracellular vesicles from zebrafish (Danio rerio) liver (ZFL), rainbow trout (Oncorhynchus mykiss) liver (RTL-W1), gill (RTgill-W1) and intestinal epithelial (RTgutGC) cell lines using stepwise centrifugation and characterized the size and morphology of EVs. Here we demonstrated that large and small extracellular vesicles can be successfully isolated using stepwise centrifugation from the serum-free medium of the selected piscine cell lines after a 24-h incubation period. The size distribution of large and small EVs isolated from the piscine cell lines suggest that large and small EV groups show high diversity in size ranges, containing heterogenous subpopulations in sizes, and the results highly depend on the applied method and whether filtration steps were included following the isolation. The spherical morphology of EVs was verified by transmission electron microscopy.

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