Serum from patients with various thrombopoietic disorders alters terminal cytoplasmic maturation of human megakaryocytes in vitro

Straneva, John E.; Briddell, Robert A.; Hui, Siu L.; Hoffman, Ronald

doi:10.1111/j.1600-0609.1989.tb00115.x

Cited by 6 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine why the number of MK colonies was increased in ITP, we aimed to define the capacity of sera of ITP patients to enhance megakaryocytopoiesis. Such an effect has already been reported by other authors in thrombocytopenia with a decreased megakaryocytic mass (13,14). In this study we also notice such an effect in thrombocytopenia of peripheral origin where the megakaryocytic mass is increased; however, we note that its intensity remains rather moderate.…”

Section: Discussionsupporting

confidence: 88%

Studies of in vitro megakaryocytopoiesis in adult immune thrombocytopenic purpura (ITP)

Bellucci¹,

Han²,

Caen³

1991

European J of Haematology

View full text Add to dashboard Cite

In vitro megakaryocytopoiesis was studied in 8 patients with chronic immune thrombocytopenia (ITP). A significant increase of megakaryocyte (MK) colony formation was observed in 5/5 patients studied. Furthermore, the serum of these 8 patients was able to enhance MK colony formation by normal marrow cells. This effect was neither due to a decrease of inhibitors of megakaryocytopoiesis such as betathromboglobulin ((3TG) nor to the IgG fraction of patients' serum. In addition, the level of interleukin 6, which is above all a stimulus for MK maturation, was found within the normal range in 8/8 patients tested. These data suggest that in chronic ITP there is an increase of MK progenitor cell number which may be due to an increased level of MK colony‐stimulating activity.

show abstract

Section: Discussionsupporting

confidence: 88%

Studies of in vitro megakaryocytopoiesis in adult immune thrombocytopenic purpura (ITP)

Bellucci¹,

Han²,

Caen³

1991

European J of Haematology

View full text Add to dashboard Cite

show abstract

“…Studies carried out on rodents indicated that AChE is secreted into the demarcation membranes and extracellular spaces of megakaryocytes just before they disintegrate to form platelets. [46] The level of AChE could therefore control megakaryocytopoiesis, perhaps in a way similar to its role in neurotransmission.…”

Section: Resultsmentioning

confidence: 99%

Effect of coumaphos on cholinesterase activity, hematology, and biochemical blood parameters of bovines in tropical regions of Mexico

Pardío

Ibarra

Waliszewski

et al. 2007

Journal of Environmental Science and Health, Part B

View full text Add to dashboard Cite

To assess the effect of coumaphos [O-(3-chloro-4-methyl-2-oxo-2H-1-benzopyran-7-yl) O,O-diethyl phosphorothioate] exposure on physiological responses during bovine production, acetylcolinesterase (AChE) and butyrylcholinesterase (BuChE) activities were measured in whole blood, erythrocytes, and plasma of healthy male steers (Bos Taurus x Bos indicus) sprayed with coumaphos at a non-lethal dose of 1 mg kg(- 1) body weight per day once every 14 (in vivo group) or 21 days (southern and central groups). Coumaphos topically administered at 1 mg/kg body weight per day to cattle under normal management practices in tropical areas produced a significant inhibition in erythrocyte (RBC) AChE and BuAChE activities when compared to baseline levels. RBC-AChE activity for the in vivo group decreased 71.3% (P < 0.05) and BuChE activity 59.1% (P < 0.05); RBC-AChE activity decreased 55.1% (P < 0.05) (southern group) and 43.4% (P < 0.05) (central group). Compared to the control specimens, steers from in vivo, southern, and central groups after 150 days of exposure had lower (P < 0.05) leukocyte count, absolute lymphocyte, erythrocyte, and platelet counts. Decreases in RBC-AChE activities correlated with decreased lymphocyte (r = 1.000, p = 0.01), erythrocyte (r = 1.000, p = 0.003), and platelet counts (r = 0.841, p = 0.036). Significantly increased BUN levels (P < 0.05) correlated with the decrease in RBC-AChE activities (r = - 0.997, p = 0.047) and with the decrease in absolute red blood cell (r = - 0.883, p = 0.020) and lymphocyte (r = - 0.825, p = 0.043) counts; increased (P < 0.05) total plasma protein levels correlated with the decrease in RBC-AChE activities (r = -0.998, p = 0.043), absolute red blood cell (r = - 0.998, p = 0.040), lymphocyte (r = - 0.893, p = 0.017), and platelet (r = -0.855, p = 0.030) counts. The physiological responses correlated with the erythrocyte acetylcholinesterase inhibition could be considered as early indicators or warning responses of bovine exposures to organophosphorus pesticides (OPs).

show abstract

Population heterogeneity among cells of the megakaryocyte lineage

Long

1993

STEM CELLS

View full text Add to dashboard Cite

Understanding the developmental steps in megakaryocyte differentiation requires information regarding the microenvironmental influences which direct or permit the growth and differentiation of these cells. The megakaryocyte microenvironment, like other lineages, is a complex structure comprised of the various megakaryocytic cells, the extracellular matrix (ECM) surrounding them, and the hematopoietic stromal cells which elaborate both growth factors and ECM. As a result, definition of the minimal essential requirements for megakaryocyte development is difficult. The intricacies of megakaryocyte development are further complicated by the cellular heterogeneity of both mature megakaryocytes and their precursors, as well as a differential responsiveness of these cells to hematopoietic growth factors. This review focuses on defining the various subpopulations of megakaryocytic cells and examining their functional distinctions and in vitro responsiveness to various stimuli.

show abstract

Serum from patients with various thrombopoietic disorders alters terminal cytoplasmic maturation of human megakaryocytes in vitro

Cited by 6 publications

References 24 publications

Studies of in vitro megakaryocytopoiesis in adult immune thrombocytopenic purpura (ITP)

Studies of in vitro megakaryocytopoiesis in adult immune thrombocytopenic purpura (ITP)

Effect of coumaphos on cholinesterase activity, hematology, and biochemical blood parameters of bovines in tropical regions of Mexico

Population heterogeneity among cells of the megakaryocyte lineage

Contact Info

Product

Resources

About