Serum Hepcidin In Patients With Chronic Hepatitis C And Its Relation To Treatment With Antiviral Drugs

Ali, Mohamed Khamis; Sayed, Ashraf Abdel Monem; Elhawary, Mostafa Abdel Aziz; Assem, Ahmed Ali Ali

doi:10.21608/aimj.2020.28247.1201

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Hepcidin deficiency causes hereditary hemochromatosis, characterized by body iron overload that may progress into liver cirrhosis (6). In addition, low hepcidin-25 concentration can be induced in iron-loading anemias and chronic hepatitis C (7,8). In contrast, hepcidin synthesis is induced by inflammation and infection (9).…”

Section: Introductionmentioning

confidence: 99%

Verification of the Automated ELISA Assay for Hepcidin-25 in Human Serum

Rolić

Mandić

Lukić

et al. 2023

Southeast. Eur. med. j. (Online)

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Introduction: Hepcidin-25, the bioactive form of hepcidin, is the master protein in regulating iron homeostasis. Serum concentrations, measured by different methods, are often incomparable and complicate results interpretation.Materials and Methods: The aim was to verify the first fully automated enzyme-linked immunosorbent assay (ELISA) method, using the DRG Hybrid XL analyzer (DRG Instruments, Marburg, Germany) standardized against the mass spectrometry method. Intra- (CVi) and inter-assay (CVg) precision and bias were performed using commercially available controls with low (C1) and high (C2) concentrations. The reference interval was verified by analyzing serum samples of 20 healthy males.Results: CVi = 9.1% (C1), 4.5% (C2); CVg = 8.9% (C1), 5.6% (C2); calculated bias was 33% for C1 and 20% for C2, respectively.Conclusion: Verification of the fully automated ELISA method for hepcidin-25 in serum on the DRG Hybrid XL analyzer met the analytical acceptance criteria.

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Section: Introductionmentioning

confidence: 99%

Verification of the Automated ELISA Assay for Hepcidin-25 in Human Serum

Rolić

Mandić

Lukić

et al. 2023

Southeast. Eur. med. j. (Online)

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“…Several studies have shown that serum markers of iron (e.g. transferrin, ferritin and iron) and liver iron accumulation have positive relation with CHC [8][9] . However, the diagnosis of cirrhosis of the liver with CHC is important as liver fibrosis is most commonly seen in stages III and IV.…”

Section: Introductionmentioning

confidence: 99%

Assessing Responsiveness of Elevated Serum Ferritin for Treatment in Chronic Hepatitis C Infected Patients

Iqbal¹,

Baig²,

Ali³

et al. 2021

PJMHS

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Background: Hepatitis C virus (HCV) infection is a foremost community health issue globally. There is anoptimisticrelationamid iron accumulation in hepatocytes and high serum markers counting transferrin and ferritin. The purpose of this analysis was to examine the responsiveness of the treatment with elevated serum ferritin in patients with hepatitis C infection. Methods: The study included 200 HCV-infected patients from the department of Medicine, Divisional Headquarters teaching hospital, Mirpurand Isra Medical University, Karachi for six months duration from 16thJune 2020 to 15thDecember 2020. The clinical feature assesses HCV exposure in all patients, biochemical data, and iron status parameters. The results were quantified using Microsoft Excel 2013. Results: The obtained outcomes indicate that antiviral treatment significantly interferes the iron accumulation in hepatocytes in HCV positive patients. All positive iron values were calculated as 19.93 mol / L; and for all negative PCR, the value was calculated at 24.61 μmol / L prior to drug started. Our patients’ levels of iron were 62.77 μmol / L in patients whose PCR is positive for HCV which is within the normal range. Reduced ferritin levels have negative effects after drug administration. The increase in ALT was observed in 37.62% of cases. Conclusions: Patients with chronic HCV have strong association with serum iron concentration. Keywords: Ferritin levels, Drugs, Iron load, Hepatitis C Virus Infection, Serum and Liver Cells.

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Serum hepcidin levels in chronic liver disease: a systematic review and meta-analysis

Sharma

Zhao

Zafar

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives Dysregulation of hepcidin-iron axis is presumed to account for abnormal iron status in patients with chronic liver disease (CLD). Our aim is to determine the effect of specific etiologies of CLD and of cirrhosis on serum hepcidin levels. Methods Pubmed, Embase, Web of Science were searched for studies comparing serum hepcidin levels in patients with CLD to that in controls using enzyme-linked immunosorbent assay. The study was conducted in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis Guidelines. Statistical analysis was carried out with STATA using random effects model to calculate the mean difference (MD) between two groups. Results Hepcidin levels were significantly lower in subjects with hepatitis C virus (16 studies) [MD −1.6 (95 % CI: −2.66 to −0.54), p<0.01] and alcoholic liver disease (3 studies) [MD −0.84 (95 % CI: −1.6 to −0.07), p=0.03] than controls. Serum hepcidin was significantly higher in subjects with non-alcoholic fatty liver disease (12 studies) [MD 0.62 (95 % CI: 0.21 to 1.03), p<0.01], but did not differ in subjects with hepatitis B and controls (eight studies) [MD −0.65 (95 % CI: −1.47 to 0.16), p=0.12]. Hepcidin levels were significantly lower in patients with cirrhosis of any etiology (four studies) [MD −1.02 (CI: −1.59 to −0.45), p<0.01] vs. controls (CI: confidence interval). Conclusions Serum hepcidin levels are altered in common forms of CLD albeit not in a consistent direction. Additional study is needed to determine how changes in hepcidin levels are related to dysregulation of iron metabolism in CLD.

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Serum Hepcidin In Patients With Chronic Hepatitis C And Its Relation To Treatment With Antiviral Drugs

Cited by 3 publications

References 20 publications

Verification of the Automated ELISA Assay for Hepcidin-25 in Human Serum

Verification of the Automated ELISA Assay for Hepcidin-25 in Human Serum

Assessing Responsiveness of Elevated Serum Ferritin for Treatment in Chronic Hepatitis C Infected Patients

Serum hepcidin levels in chronic liver disease: a systematic review and meta-analysis

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