Serum Hepcidin Levels in Bulgarian Population

Manolov, V.; Atanasova, Б.; Velizarova, M.; Vasilev, V.; Tzatchev, К.

doi:10.7754/clin.lab.2014.140322

Cited by 9 publications

(10 citation statements)

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“…This suggests that hepcidin rise in SAT is mainly due to an acute-phase reaction, less as a reflection of accompanying Fe homeostasis imbalance. Our results are supported by the observation made on Bulgarian population that serum concentrations ferritin and hepcidin correlate significantly [27].…”

Section: Discussionsupporting

confidence: 90%

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Hepcidin and Iron Homeostasis in Patients with Subacute Thyroiditis and Healthy Subjects

Hernik

Szczepanek‐Parulska

Filipowicz

et al. 2019

Mediators of Inflammation

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Purpose. Hepcidin is an acute-phase protein involved also in regulation of iron homeostasis. The aim of the study was to prospectively assess for the first time the hepcidinEL concentration in patients with subacute thyroiditis (SAT), to identify biochemical determinants of hepcidinEL concentration and evaluate the potential role of hepcidin in SAT diagnosis and monitoring. Methods. Out of 40 patients with SAT initially recruited, restrictive inclusion criteria fulfilled 21 subjects aged 45±10 years and 21 healthy control subjects (CS). HepcidinEL concentration, thyroid status, and iron homeostasis were evaluated at SAT diagnosis and following therapy and compared with CS. Results. The median hepcidinEL concentration at SAT diagnosis is higher than that in CS (48.8 (15.9-74.5) ng/mL vs. 18.2 (10.2-23.3) ng/mL, p=0.009) and is significantly lower after treatment (4.0 (1.2-10.0) ng/mL, p=0.007) compared with CS. The ROC analysis for hepcidinEL at SAT diagnosis revealed that area under the curve (AUC) is 0.735 (p=0.009), and the cut-off for hepcidinEL concentration is 48.8 ng/mL (sensitivity 0.52 and specificity 0.95). HepcidinEL in SAT patients correlated with CRP (r=0.614, p=0.003), ferritin (r=0.815, p<0.001), and aTPO (r = -0.491, p=0.024). On multiple regression, the correlation between hepcidinEL and ferritin was confirmed (p<0.001). Conclusions. SAT is accompanied by a significant increase in hepcidin, which reflects an acute-phase inflammatory process. Parameters of iron homeostasis improved significantly while inflammatory indices got lower following recovery. The potential role of hepcidin as a predictive factor of the risk of SAT relapse needs to be assessed in studies on larger groups of SAT patients.

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Section: Discussionsupporting

confidence: 90%

“…According to the manufacturer, the normal range for serum hepcidin measured by ELISA is 0.2-47.7 ng/mL. Previous research estimated a similar reference range 3.1-37.7 ng/mL for healthy population [27]. In our study, the mean ± SD values for hepcidin in healthy CS were 18.7 ± 14.3 ng/mL, being significantly lower than that in SAT patients.…”

Section: Discussionsupporting

confidence: 58%

Hepcidin and Iron Homeostasis in Patients with Subacute Thyroiditis and Healthy Subjects

Hernik

Szczepanek‐Parulska

Filipowicz

et al. 2019

Mediators of Inflammation

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“…We quantify serum hepcidin levels using verified ELISA method [12] from previous studies. Atomic Absorption Spectrometry (AAS) was used for quantification of serum iron and total iron binding capacity (TIBC).…”

Section: Methodsmentioning

confidence: 99%

Serum Hepcidin Quantification in Stroke in β-Thalassemia and Rheumatoid Arthritis Patients

Manolov¹

2017

Austin J Cerebrovasc Dis & Stroke

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Background and Purpose: Anemia occurs often in different conditions, associated with iron metabolism and flowing in a number of diseases. Variations in serum levels of iron is due to a number of reasons, such as bleeding from the gastrointestinal tract, inflammation, production of inflammatory cytokines, deposition of iron in various tissue structures, and etc. Oxidative stress plays an important role in neuronal injuries caused by cerebral ischemia. We aimed to quantify serum hepcidin in rheumatoid arthritis patients with ischemic stroke and β-thalassemia with stroke and evaluate peptide changes in combination of two diseases.Methods: For a period of two years 138 patients with ischemic stroke were enrolled. They were separated into three groups -49 with ischemic stroke, caused by vascular reasons (ISVR), 43 patients with rheumatoid arthritis (RA) and 46 β-thalassemia cases (BTM). Their results were compared to age and gender matched healthy controls. Established parameters from rheumatoid arthritis and β-thalassemia cases were compared with RA and BTM patients without stroke. Statistical analysis of established results was performed using Pearson's correlation and Student's paired t-test. Results:We found statistically different results from included groups for serum hepcidin levels. They differ in case if an acute stroke occurs during rheumatoid arthritis or β-thalassemia. Serum hepcidin levels statistically differ in three ischemic stoke groups: ISVR 87.6±9.4 µg/L, RA 19.4±0.9 µg/L and BTM 7.9±1.1 µg/L (P<0.001). In patients with RA and BTM without stroke hepcidin shows serum concentrations from 35.6±1.4 µg/L and 0.95±0.3 µg/L (P<0.001). In healthy controls we quantified serum hepcidin as 22.7±5.1 µg/L. Conclusion:Our study in patients with acute stroke, rheumatoid arthritis and stroke and β-thalassemia and stroke confirms the ability of verified immunochemical method for evaluation of iron homeostasis. It provides a basis for the correct choice of therapeutic approach in anemia treatment. Rheumatoid arthritis increases serum hepcidin levels in stroke; in patients with β-thalassemia peptide's concentration decreases. Dissimilar diseases are accompanied with iron disorders, thereby masking the state of this trace element in human body.

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“…Characterization of iron metabolism parameters was determined using an automatic biochemical analyzer Cobas Integra 400 (of Roche Diagnostics) and Advia 2120 hematology analyzer (Siemens Healthcare Diagnostics). We used the verified ELISA method to quantify serum hepcidin levels [5,6]. Data were analyzed by the Student t-test.…”

Section: Methodsmentioning

confidence: 99%

Serum Hepcidin Evaluation in Patients with Chronic Dialysis

Manolov¹,

Yonova²,

Vazelov³

et al. 2015

Int J Biomed

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The aim of our study was to quantify serum hepcidin levels in Bulgarian patients with chronic kidney disease (CKD). Expected high values of peptide hormones might provide a new therapeutic choice for anemia of chronic disease. We looked for correlation between serum hepcidin levels and some iron metabolism parameters in CKD patients.Results: The sandwich ELISA is highly specific for hepcidin-25. We found statistically significant differences in serum hepcidin levels in patients of the control group, with CKD stages II to IV and CKD stage V (on chronic dialysis): 12.7±8.7μg/L, 90.74±21.1μg/L, and 282.49±81.1μg/L, respectively. Significant correlation between serum hepcidin and transferrin saturation (r=0.340, P<0.05) was found in the group of patients with CKD stage V.Conclusion: The use of 2 monoclonal antibodies in a sandwich ELISA format provides a reliable, reproducible, and inexpensive method for measuring serum concentrations of the bioactive form of hepcidin in Bulgarian laboratory practice.

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Serum Hepcidin Levels in Bulgarian Population

Cited by 9 publications

References 0 publications

Hepcidin and Iron Homeostasis in Patients with Subacute Thyroiditis and Healthy Subjects

Hepcidin and Iron Homeostasis in Patients with Subacute Thyroiditis and Healthy Subjects

Serum Hepcidin Quantification in Stroke in β-Thalassemia and Rheumatoid Arthritis Patients

Serum Hepcidin Evaluation in Patients with Chronic Dialysis

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