Serum hyaluronate reflects hepatic sinusoidal capillarization

Ueno, Takato; Inuzuka, Sadataka; Torimura, Takuji; Tamaki, Seishu; Koh, Houhi; Kin, Motoaki; Minetoma, Tomoaki; Kimura, Yoshio; Ohira, Hiromasa; Sata, Michio; Yoshida, Hiroshi; Tanikawa, Kyuichi

doi:10.1016/0016-5085(93)90722-o

Cited by 106 publications

(59 citation statements)

References 22 publications

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“…The high level of hyaluronic acid may also be due to decrease in its degradation by hyaluronidase's enzymes (Fraser et al 1997). Similar to our study, Ueno et al (1993) reported that serum hyaluronic acid reflects the morphological and functional changes in the sinusoidal endothelial cells that accompany hepatic sinusoidal capillarization in various liver disorders. Comparing serum hyaluronic acid levels with histological fibrosis in children with biliary atresia, Kobyashi et al (1999) and Hasegawa et al (2000) showed that significant fibrosis correlated with increased hyaluronic acid levels.…”

Section: Discussionsupporting

confidence: 91%

Dimethylnitrosamine (DMN)-induced fibrotic rats: effect of Vernonia amygdalina on extracellular matrix and Hepatic/lysosomal integrity

Usunobun

Okolie

2016

IJPT

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Background: Hepatic fibrosis is the accumulation of extracellular matrix protein, or scar, in response to acute or chronic liver injury. This study investigated the effect of ethanolic leaf extract of Vernonia amygdalina on extracellular matrix protein: hyaluronic acid (HA), liver synthetic molecules: total protein (TP), albumin (ALB), total bilirubin (TB) as well as lysosomal membrane stability: acid phosphatase (ACP) in dimethylnitrosamine (DMN)-induced fibrotic rats. Methods: Wistar albino male rats were intraperitoneally injected with 10 mg/kg DMN on first three days a week for two weeks. Ethanolic leaf extracts of Vernonia amygdalina (200 mg /kg) was administered simultaneously by oral gavage daily for two weeks. All rats were sacrificed after 24 hours of last administration by cardiac puncture, and blood collected from the ocular vein. Analysis of serum ACP and LDH activities with those of the concentrations of HA, ALB, TP and TB were carried out. Results: Administration of DMN to rats significantly increased HA and TB concentration and the activities of ACP and LDH (p<0.05) in the serum while it significantly reduced (p<0.05) serum TP and ALB concentrations when compared with controls. However, simultaneous administration of ethanolic leaf extracts of Vernonia amygdalina with DMN significantly (p<0.05) reversed these changes. Conclusion: This study shows that Vernonia amygdalina possesses hepatoprotective, lysosomal membrane stabilizing and anti-fibrotic properties may be due to its antioxidant and phytochemical constituents.

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Section: Discussionsupporting

confidence: 91%

Dimethylnitrosamine (DMN)-induced fibrotic rats: effect of Vernonia amygdalina on extracellular matrix and Hepatic/lysosomal integrity

Usunobun

Okolie

2016

IJPT

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“…The concentration of HA in blood increases as fibrosis develops in patients with liver disease, especially in patients with liver cirrhosis [7,10,11,13,14]. Although the mechanism underlying this elevation in HA concentration is not fully understood, the synthesis of HA in activated hepatic stellate cells, the main fibrogenic cells in the diseased liver, and the reduced uptake of HA by the hepatic sinusoidal endothelial cells may play an important role [5,8,14,15]. As the structure of HA is conserved among different species, the assay system used to measure human HA can be used to measure the HA of various species, including dogs [3,18].…”

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confidence: 99%

“…These results indicated that increased HA in the liver was one of the reasons of elevated circulating HA. However, it was also possible that other factors such as impaired incorporation and degradation of HA from circulation to the liver [8,14,15] affects blood HA concentration.…”

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confidence: 99%

Blood Hyaluronic Acid as a Marker for Canine Cirrhosis

Kanemoto

Ohno

Sakai

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Blood hyaluronic acid (HA) concentration was measured in dogs with various liver diseases to determine its relationship with histological fibrosis of the liver. The blood HA concentration significantly increased in dogs with chronic liver diseases compared with extrahepatic diseases and control. Furthermore, the median blood HA concentration in dogs with liver cirrhosis (500 g/l; range, 151-1970 g/l) was significantly higher than dogs with non-cirrhotic liver diseases (153 g/l; range, 15-477 g/l). In histochemical analysis, HA was distributed primarily in the fibrotic area in dogs with chronic liver diseases. As a conclusion, the blood HA concentration was significantly increased in dogs with chronic liver diseases, especially those with cirrhosis. Measurement of the blood HA levels of dogs with suspected liver disease can be a useful diagnostic aid for canine cirrhosis.KEY WORDS: canine liver, fibrosis, hyaluronic acid.

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“…The determination of serum HA levels is useful for assessing the degree of liver fibrosis and for monitoring liver function [13][14][15][16] and thus is a topic of high interest for the treatment of patients in our departmental hepatology outpatient clinic. Monitoring serum HA is also used in other areas, such as in septic conditions, tumor growth (such as Wilm's tumor and malignant mesothelioma), and for the diagnosis of rare hereditary diseases (such as Werner's syndrome and cutaneous hyaluronanosis) [4].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the disaccharides derived from hyaluronic acid and chondroitin sulfate by capillary electrophoresis with sample stacking

Yang

Breadmore

Thormann³

2005

J of Separation Science

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Analysis of the disaccharides derived from hyaluronic acid and chondroitin sulfate by capillary electrophoresis with sample stacking CE conditions for monitoring the unsaturated disaccharides of hyaluronic acid (di-HA) and chondroitin sulfate (di-CS) using an alkaline tetraborate buffer, electrokinetic sample injection, and UV absorption detection at 232 nm are reported. Separations were performed in an uncoated fused-silica capillary having reversed polarity and reversed electroosmosis generated with the addition of CTAB to the buffer. The influence of various separation parameters, including the concentration of CTAB, buffer pH, concentration of tetraborate, and applied voltage, on the resolution of the two disaccharides was investigated. Baseline separation was obtained with 25 mM tetraborate at pH 10.0 and having 0.05 mM CTAB. Chloride and phosphate in the sample are beneficial for the stacking of the disaccharides, with di-HA forming a much sharper peak than di-CS. Using samples prepared in 25 mM Tris-HCl (pH 7.5) and electrokinetic injection at the cathode at -10 kV for 40 s, linear relationships between the corrected peak area and the concentration of the disaccharides have been found in the ranges of 1.0-400.0 and 0.1-1.0 lg/mL (0.2-1.0 lg/mL for di-CS), with correlation coefficients being A0.9933 in all cases. The RSDs of detection times and corrected peak areas were between 1.13-1.24 and 1.57-2.13%, respectively. Applied to human serum samples that were prepared by ethanol precipitation and depolymerization of the two polysaccharides with chondroitinase ABC reveals comigration of endogenous compounds with di-HA and a sample-dependent detection time. The di-HA content in the serum sample can be estimated via subtraction of the blank peak that is obtained without enzymatic hydrolysis.

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Serum hyaluronate reflects hepatic sinusoidal capillarization

Cited by 106 publications

References 22 publications

Dimethylnitrosamine (DMN)-induced fibrotic rats: effect of Vernonia amygdalina on extracellular matrix and Hepatic/lysosomal integrity

Dimethylnitrosamine (DMN)-induced fibrotic rats: effect of Vernonia amygdalina on extracellular matrix and Hepatic/lysosomal integrity

Blood Hyaluronic Acid as a Marker for Canine Cirrhosis

Analysis of the disaccharides derived from hyaluronic acid and chondroitin sulfate by capillary electrophoresis with sample stacking

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