Serum immunoglobulin from Nellore cattle produced by in vitro fertilization and treated for umbilical diseases

Abstract

The aim of this study was to measure serum immunoglobulin concentrations of Nellore cattle produced by in vitro fertilization (IVF) with umbilical diseases and to evaluate surgical excision as a method of treatment. Sixteen cattle with ages ranging from 1 to 15 months, males and females, affected by umbilical diseases were enrolled in the study. Blood samples were collected for cell counts and the determination of immunoglobulin concentrations by electrophoresis and zinc sulphate turbidimetry (ZST). Four calves were presented with umbilical herniation, two with an umbilical herniation associated with a persistent urachus, two with an umbilical herniation with a persistent urachus and omphaloarteritis, three with an umbilical herniation and an urachal diverticulum, three with a persistent urachus, one with an urachal diverticulum, and one with omphalitis. The blood cell counts pre- and post-surgical revealed differences in cell volume and the number of leukocytes. The immunoglobulin values measured by electrophoresis values were below normal in most animals, whereas the ZST showed normal levels in most of them. Most of the calves affected by umbilical diseases and produced by IVF presented hypoglobulinaemia. Correlations between umbilical diseases, failure of passive transfer of immunity and IVF could not be demonstrated.

Abnormalities of Serum Protein Electrophoresis in Cattle and Comparison between Assays for Markers of Inflammation and Markers of Liver Alteration

“…Elsewhere, it is caused by the decrease of albumin may refer to weight loss (transition cow period), a consequence of malnutrition (insufficient types of diets). Moreover, the decrease of albumin is considered a negative marker of inflammation (Piccione et al2012;Rodrigues et al 2018). Moreover, the subclinical pathology with the chronic evolution had a peculiar effect on the bovine gamma globulin (Jacobsen et al 2004).…”

Clinical and surgical approach to umbilical disorders in calves - literature review

“…Iodine tincture 7%, and chlorhexidine gluconate 2% and 4% have been successfully used to prevent umbilical infections in calves (Robinson et al, 2015;Wieland et al, 2016). Some calves display a predisposition to umbilical disorders, which may arise from genetic factors or failure to transfer passive immunity; unhygienic conditions may be another possible reason (Rodrigues et al, 2018). Conception methods may be correlated with the occurrence of omphalopathies: animals from in vitro fertilization had a higher frequency of urachus persistence (66.7%), and those conceived by artificial insemination had a higher frequency of umbilical hernia (58.4%) (Rodrigues et al 2010).…”