Serum increases the CD13 receptor expression, reduces the transduction of fluid-mechanical forces, and alters the metabolism of HL60 cells cultured in agitated bioreactors

McDowell, Christi L.; Papoutsakis, Eleftherios T.

doi:10.1002/(sici)1097-0290(19981020)60:2<259::aid-bit13>3.0.co;2-h

Cited by 15 publications

(6 citation statements)

References 29 publications

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“…However, apoptosis is a major concern in using low‐serum and serum‐free media. As shown in Figure 9, decreasing the serum content increased apoptosis in both 2‐D and 3‐D cultures, which is consistent with previous reports that serum can protect cells from apoptotic death and help to maintain culture viability (53, 54). However, the 3‐D culture had a much lower apoptotic rate than the 2‐D culture, especially at 0% serum.…”

Section: Resultssupporting

confidence: 91%

Effects of Three-Dimensional Culturing in a Fibrous Matrix on Cell Cycle, Apoptosis, and MAb Production by Hybridoma Cells

Luo

Yang

2008

Biotechnol Progress

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The effects of culturing hybridoma cells in a three-dimensional (3-D) poly(ethylene terephthalate) (PET) fibrous matrix on cell cycle, apoptosis, metabolism, and monoclonal antibody (MAb) production were evaluated by comparing with two-dimensional (2-D) culturing on microcarrier and multiwell plate surfaces. The percentage of cells in the G1/G0 phase increased during the long-term culturing period of approximately 4 weeks. Compared to the 2-D culture systems, cells grown in 3-D matrices had higher MAb productivity for long-term culture. Decreasing serum content in the culture medium increased both MAb productivity and apoptosis. However, the 3-D culture had a greater increase in MAb productivity and a much lower apoptotic rate than the 2-D culture, especially at 0% serum. Most cells in the 3-D fibrous matrix formed large aggregates and were smaller than cells grown on a 2-D surface or in suspension. The smaller cell size allowed cells to survive better in the high-cell-density environment. The fibrous matrix also selectively retained healthy, nonapoptotic cells. These results suggested that the 3-D fibrous matrix contributed to growth arrest, protected cells to better resist low-serum environments, and reduced apoptosis, all of which contributed to the high viable cell density and volumetric MAb productivity in the long-term 3-D culture.

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Section: Resultssupporting

confidence: 91%

Effects of Three-Dimensional Culturing in a Fibrous Matrix on Cell Cycle, Apoptosis, and MAb Production by Hybridoma Cells

Luo

Yang

2008

Biotechnol Progress

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“…In this regard, the change observed in the CH 2 /CH 3 ratio must not be necessarily imputed to apoptosis. The molecular mechanism underlying the CH 2 /CH 3 ratio increase may be related to a slight decrease in cellular volume following extensive growth conditions [McDowell and Papoutsakis, ]. In fact, a decrease in the cellular volume must not necessarily be related to a decrease of the surface area.…”

Section: Resultsmentioning

confidence: 99%

Effects of low intensity static magnetic field on FTIR spectra and ROS production in SH‐SY5Y neuronal‐like cells

Calabrò

Condello

Currò

et al. 2013

Bioelectromagnetics

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Biological effects of man-made electromagnetic fields (EMFs) have been studied so far by experimental approaches exposing animals and cell cultures to EMFs. However, the evidence for cell toxicity induced by static magnetic field (SMF) is still uncertain. We investigated the effects produced by the exposure of human SH-SY5Y neuronal-like cells to a uniform magnetic field at intensities of 2.2 mT, which is less than the recommended public exposure limits set by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). A decrease of membrane mitochondrial potential up to 30% was measured after 24 h of exposure to SMF in SH-SY5Y cells, and this effect was associated with reactive oxygen species production increase. Fourier transform infrared spectroscopy (FTIR) analysis showed that exposure to a static magnetic intensity around 2.2 mT changed the secondary structure of cellular proteins and lipid components. The vibration bands relative to the methylene group increased significantly after 4 h of exposure, whereas further exposure up to 24 h produced evident shifts of amide I and II modes and a relative increase in β-sheet contents with respect to α-helix components. Our study demonstrated that a moderate SMF causes alteration in cell homeostasis, as indicated by FTIR spectroscopy observations of changes in protein structures that are part of cell response to magnetic field exposure.

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“…These findings probably indicated that with respect to oxidative stress [26], fatty acid metabolism, evaluated by change in the ν as (CH 3 ) absorbance [26], was changing in apoptotic Jurkat cells. It was reported that the tendency in the increase in the CH 2 /CH 3 absorbance ratio might depends by cell growth conditions [3,14,20,21,33]. Because apoptosis lead to cell shrinkage [16], a decrease in the cellular volume should correspond to a relative increase in the surface area.…”

Section: The C-h Stretching Regionmentioning

confidence: 99%

FT-IR spectromicroscopy of mammalian cell cultures during necrosis and apoptosis induced by drugs

Lamberti¹,

Sanges²,

Arcari

2010

Spectroscopy

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Abstract. FT-IR is used in the field of biology and medicine to detect bimolecular changes in disordered cells and tissues. In this report, using FT-IR microscopy, we characterize changes in apoptotic and necrotic Jurkat cells with respect to normal cells. The analysis of deconvoluted regions of the FT-IR spectra showed significant differences compared to the controls in three spectral regions. In particular, the apoptotic cells were characterized by an increase in the absorption at 2925 cm −1 , due to the asymmetric CH 2 -stretching (νasCH 2 ) of membrane lipids whereas the spectral areas ratio (A 1654 /A 1629 ) of the amide I region indicated an increase in apoptotic cells of more α-helical structures with respect to of β-sheet content. Interestingly, apoptotic cells showed the appearance of a peak around 1743 cm −1 , ν(C=O) assigned to acid ester. Because no other similar increase for lipid bands was observed, the increase of A 1745 is not simply due to an increase in the number of lipid molecules or their density but could also be indicative as marker of apoptosis. These spectral changes were not observed in necrotic Jurkat cells.

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Serum increases the CD13 receptor expression, reduces the transduction of fluid-mechanical forces, and alters the metabolism of HL60 cells cultured in agitated bioreactors

Cited by 15 publications

References 29 publications

Effects of Three-Dimensional Culturing in a Fibrous Matrix on Cell Cycle, Apoptosis, and MAb Production by Hybridoma Cells

Effects of Three-Dimensional Culturing in a Fibrous Matrix on Cell Cycle, Apoptosis, and MAb Production by Hybridoma Cells

Effects of low intensity static magnetic field on FTIR spectra and ROS production in SH‐SY5Y neuronal‐like cells

FT-IR spectromicroscopy of mammalian cell cultures during necrosis and apoptosis induced by drugs

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