Serum-induced collagen gel contraction

Background: To evaluate the role of the vitreous in diabetic eyes, autopsy eyes from diabetic patients have been examined by means of light and electron microscopy. Methods: Twenty-five eyes were obtained from 19 patients. The duration of diabetes was from 8 to 26 years (average 17 years). All patients had type II diabetes. The glycerol-dehydrated globes were embedded in celloidin and hardened with chloroform. For light microscopy, areas of interest were cut from 200-μm-thick celloidin sections and embedded in paraffin, and 10-μm sections were cut. Such 200-μm and 10-μm sections were also used for scanning electron microscopy. For transmission electron microscopy, areas of interest were cut from thick celloidin sections and embedded in Epon for thin sectioning. Results: The vitreous in diabetic eyes exhibits delayed senile degeneration in case of early onset of diabetes. It shows intensified staining of the vitreous cortex. Posterior detachment is often incomplete without collapse. The vitreous collagen fibers are coarse and aggregated in the centrally located cortical region of the vitreous. Hyalocytes in diabetic eyes have a different shape than normally, and their number seems to be increased. All these structural changes were not demonstrated in nondiabetic eyes. Conclusion: The vitreous in diabetic patients is morphologically different from normal vitreous. Features of the diabetic vitreous are aggregated collagen fibers, increased density of the vitreous cortex and morphological changes of hyalocytes. It is discussed whether these findings arise from nonenzymatic glycosylation of collagen molecules resulting in diabetic vitreopathy.

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“…Serum-induced collagen contraction may also contribute to contraction of the vitreous cortex in diabetes [24].…”

Section: Discussionmentioning

confidence: 99%

Diabetic Vitreopathy – Findings Using the Celloidin Embedding Technique

Faulborn

Dunker²,

Bowald³

1998

Ophthalmologica

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“…In this model, fibroblasts can reach a high degree of differentiation, exhibiting a bipolar morphology, controlled cell division, and regulated synthesis of macromolecules. Reorganization of the collagen gels by fibroblasts propagated throughout the interconnected collagen's fibril network (10). Previous studies have shown that DNA synthesis and collagen synthesis by fibroblasts decreased after contraction of collagen gel (11).…”

mentioning

confidence: 94%

Tissue Engineered Artificial Skin Composed of Dermis and Epidermis

et al. 2000

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We made an artificial skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the artificial skin, 1x10(5) cells/cm2 of keratinocytes were cultured on the dermal equivalent at air-liquid interface. Finally, to overcome the problem that artificial skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured artificial skins were successfully grafted onto rats.

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“…After an eye injury, the intraocular structures change, and the blood-retinal barrier is broken. The blood components and cell mediation lead to vitreous shrinkage, causing PVD [22–24]. Therefore, with a greater delay in performing vitrectomy after the injury, fewer vitreous remnants remain.…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Residual Vitreous Cortex at the Fovea after Posterior Vitreous Detachment during Vitrectomy in Ocular Trauma

Wang

Zhao

et al. 2019

Journal of Ophthalmology

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Purpose. To investigate the frequency and patterns of the residual vitreous cortex (RVC) at the fovea after posterior vitreous detachment (PVD) during vitrectomy after ocular trauma and the risk factors associated with its occurrence. Methods. A cross-sectional, multicenter, and observational study was conducted in 72 consecutive patients who underwent pars plana vitrectomy after ocular trauma. In patients with PVD after vitrectomy, RVC patterns were visualized using triamcinolone acetonide staining and were classified as diffuse or focal. A multivariate logistic regression analysis was performed to evaluate the association between RVC and various factors, such as the patient’s age, preoperative visual acuity, days after injury, the existence of spontaneous PVD, and the type of injury prior to vitrectomy. Results. Of the 72 eyes with trauma, 35 eyes (48.6%) showed RVC on the macula, of which 19 (54.3%) were of the diffuse type and 16 (45.7%) were of the focal type. A multivariate logistic regression analysis showed that age (OR = 0.933, P=0.025) and days after injury (OR = 0.862, P=0.013) had a significant impact on RVC. Conclusions. RVC at the fovea is very common after spontaneous or surgical PVD during vitrectomy. Younger patients are more likely to show RVC if vitrectomy is performed soon after injury.

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Serum-induced collagen gel contraction

Cited by 10 publications

References 25 publications

Diabetic Vitreopathy – Findings Using the Celloidin Embedding Technique

Diabetic Vitreopathy – Findings Using the Celloidin Embedding Technique

Tissue Engineered Artificial Skin Composed of Dermis and Epidermis

Risk Factors for Residual Vitreous Cortex at the Fovea after Posterior Vitreous Detachment during Vitrectomy in Ocular Trauma

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