Serum‐Induced Iron‐Acquisition Systems and TonB Contribute to Virulence in<i>Klebsiella pneumoniae</i>Causing Primary Pyogenic Liver Abscess

Hsieh, Pei-Fang; Lin, Tzu‐Lung; Lee, Cha-Ze; Tsai, Shih‐Feng; Wang, Jann-Tay

doi:10.1086/588383

Cited by 198 publications

(217 citation statements)

References 38 publications

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“…Our previous work demonstrated that PLA strains harbor a pathogenesis-specific genotype and transcriptional profile. 31 We therefore hypothesized that a novel adhesin may be produced in PLA-causing K. pneumoniae. However, our in vitro observations suggested that K. pneumoniae has a broad tissue tropism and can infect various types of human epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial strains, plasmids, and primers used in this study are listed in Table S1. 30,31,41,43,51 Gene deletion and complementation. K. pneumoniae mutated in yfgL, clpS, entS, fim, yraM, or pts were constructed using the previously described unmarked deletion method.…”

Section: Methodsmentioning

confidence: 99%

“…K. pneumoniae mutated in yfgL, clpS, entS, fim, yraM, or pts were constructed using the previously described unmarked deletion method. 31 For cis-complementation, the intact yfgL, or clpS gene was amplified by polymerase chain reaction (PCR). To generate the yfgL complementation strain, a PCR fragment amplified by primers yfgL-FR1 and yfgL-R was cloned into a pGEM-T Easy vector (Promega).…”

Section: Methodsmentioning

confidence: 99%

“…A randomly selected subgroup of genes that were highly affected by YfgL, as determined by microarray hybridization, was analyzed by real-time reverse-transcription quantitative PCR (qPCR), as described previously. 31 Fimbrial switch orientation assay. A modification of a previously described method was used to determine the orientation of the K. pneumoniae fim switch.…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we generated a NTUH-K2044 genomic library (with 3-to 5-kb inserts). 31 To identify the possible adhesion molecules of the PLA-endophthalmitisassociated K. pneumoniae strains, adherence of DH5a E. coli harboring the NTUH-K2044 genomic library was analyzed. Eleven clones showed a hyperadherent phenotype; the NTUH-K2044 DNA inserts in these clones were sequenced (Table 1).…”

Section: Identification Of the E Coli Recombinant Strains Elevated Imentioning

confidence: 99%

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TheKlebsiella pneumoniaeYfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, andin vivovirulence

et al. 2016

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Klebsiella pneumoniae is an opportunistic pathogen that causes several kinds of infections, including pneumonia, bacteremia, urinary tract infection and community-acquired pyogenic liver abscess (PLA). Adhesion is the critical first step in the infection process. Our previous work demonstrated that the transcellular translocation is exploited by K. pneumoniae strains to migrate from the gut flora into other tissues, resulting in systemic infections. However, the initial stages of K. pneumoniae infection remain unclear. In this study, we demonstrated that a K. pneumoniae strain deleted for yfgL (bamB) exhibited reduced adherence to and invasion of host cells; changed biogenesis of major b-barrel outer membrane proteins; decreased transcriptional expression of type-1 fimbriae; and increased susceptibility to vancomycin and erythromycin. The yfgL deletion mutant also had reduced ability to against neutrophil phagocytosis; exhibited decreased induction of host IL-6 production; and was profoundly attenuated for virulence in a K. pneumoniae model of bacteremia. Thus, the K. pneumoniae YfgL lipoprotein mediates in outer membrane proteins biogenesis and is crucial for anti-phagocytosis and survival in vivo. These data provide a new insight for K. pneumoniae attachment and such knowledge could facilitate preventive therapies or alternative therapies against K. pneumoniae.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Identification Of the E Coli Recombinant Strains Elevated Imentioning

confidence: 99%

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TheKlebsiella pneumoniaeYfgL (BamB) lipoprotein contributes to outer membrane protein biogenesis, type-1 fimbriae expression, anti-phagocytosis, andin vivovirulence

et al. 2016

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The making of hypervirulent Klebsiella pneumoniae

Dai

2022

Clinical Laboratory Analysis

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Klebsiella pneumoniae is a notorious bacterium in clinical practice. Virulence, carbapenem‐resistance and their convergence among K. pneumoniae are extensively discussed in this article. Hypervirulent K. pneumoniae (HvKP) has spread from the Asian Pacific Rim to the world, inducing various invasive infections, such as pyogenic liver abscess, endophthalmitis, and meningitis. Furthermore, HvKP has acquired more and more drug resistance. Among multidrug‐resistant HvKP, hypervirulent carbapenem‐resistant K. pneumoniae (Hv‐CRKP), and carbapenem‐resistant hypervirulent K. pneumoniae (CR‐HvKP) are both devastating for their extreme drug resistance and virulence. The hypervirulence of HvKP is primarily attributed to hypercapsule, macromolecular exopolysaccharides, or excessive siderophores, although it has many other factors, for example, lipopolysaccharides, fimbriae, and porins. In contrast with classical determination of HvKP, that is, animal lethality test, molecular determination could be an optional and practical method after improvement. HvKP, including Hv‐CRKP and CR‐HvKP, has been progressing. R‐M and CRISPR‐Cas systems may play pivotal roles in such evolutions. Hv‐CRKP and CR‐HvKP, in particular the former, should be of severe concern due to their being more and more prevalent.

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