Serum, insulin and phorbol esters stimulate rRNA and tRNA gene expression in both dividing and nondividing Drosophila cells

Weber, Heather W.; Vallett, S M; Neilson, Lorna; Grotke, Mark; Chao, Yesu; Brudnak, Mark A.; Juan, Alan San; Pellegrini, Maria

doi:10.1007/bf00229821

Cited by 16 publications

(2 citation statements)

References 20 publications

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“…Gene ontology (GO) based analysis showed enrichment of translation in upregulated genes, whereas a variety of other processes were overrepresented in the downregulated set ( Table 1; for a complete list and details, see Nutrient sensing signaling pathway is known to encompass rRNA transcription from Drosophila to man. [26][27][28][29] Besides growth regulators, epigenetic modifications also control rRNA transcription across species. [30][31][32][33][34][35][36][37] Ribosomal components are also suggested to be involved in gene expression including epigenetic mechanisms in higher eukaryotes including Drosophila.…”

Section: Biological Process and Pathway Enrichmentmentioning

confidence: 99%

Systems level analysis of transgenerational spermatogenic inheritance predicts biomarkers and underlying pathways

Sharma

2009

Nat Prec

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Transgenerational spermatogenic inheritance of adult male acquired CNS gene expression characteristics has recently been discovered using a Drosophila systems model. In this novel mode of inheritance, transcriptomic alteration induced by the neuroactive drug pentylenetetrazole (PTZ) has been found to leak to future generations. Here, the available microarray gene expression data pertaining to CNS and/or testis of exposed F 0 and the resulting F 1 and F 2 generations has been pooled and analyzed in an unbiased manner at four levels, namely, biological processes and pathways, protein interactome networks, miRNA-targets, and microarray expression profile similarities. Enrichment for processes related to translation, energy metabolism, cell proliferation, cell differentiation, secretion, central nervous system development, germ cell development, gamete generation, wing development, nutrition etc. was observed. Also, ribosome, oxidative phosphorylation and, to a lesser extent, wingless signaling pathway showed overrepresentation. In the proteomic interactome map, the cell cycle gene Ras85D exhibited overinteraction. In miRNA-target network, the fly transgenerational genes showed overrepresentation of mir-315 targets. Transcriptomic matching revealed overlap of transgenerational set with genes related to epigenetic drug treatment, stem cells, Myc targets and miRNA targets. Many of the findings were consistent with the existing epigenetic evidence in complex mammalian traits. Converging evidence suggests that ribosomal RNA and proteins may serve as candidate biomarkers of transgenerational environmental effect. A compelling systems biology framework integrative of transgenerational epigenetic inheritance is suggested. Nutrient, circulating peptide hormone, Myc, Wnt, and stem cell signaling pathways constitute the framework. The analysis has implications in explaining missing heritability in complex traits including common human disorders. The fly model offers an excellent opportunity to understand somatic and germline communication, and epigenetic memory formation and its retention across generations in molecular details.

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Section: Biological Process and Pathway Enrichmentmentioning

confidence: 99%

Systems level analysis of transgenerational spermatogenic inheritance predicts biomarkers and underlying pathways

Sharma

2009

Nat Prec

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“…In addition, it can substitute for calcium in protein kinase C and calmodulin to activate these proteins and subsequently affect downstream targets (24,25). Elevation of cellular calcium levels or activation of protein kinase C has been shown to increase rDNA transcription and the mRNA levels of adrenergic receptor kinases 1 and 2 (77,78). The effect of cadmium on the expression of these genes has not been determined.…”

Section: Figmentioning

confidence: 99%

Cadmium-regulated Genes from the NematodeCaenorhabditis elegans

Liao¹,

Freedman²

1998

Journal of Biological Chemistry

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The transition metal cadmium is a pervasive and persistent environmental contaminant that has been shown to be both a human toxicant and carcinogen. To inhibit cadmium-induced damage, cells respond by increasing the expression of genes encoding stress-response proteins. In most cases, the mechanism by which cadmium affects the expression of these genes remains unknown. It has been demonstrated in several instances that cadmium activates gene transcription through signal transduction pathways, mediated by protein kinase C, cAMPdependent protein kinase, or calmodulin. A codicil is that cadmium should influence the expression of numerous genes. To investigate the ability of cadmium to affect gene transcription, the differential display technique was used to analyze gene expression in the nematode Caenorhabditis elegans. Forty-nine cDNAs whose steady-state levels of expression change 2-6-fold in response to cadmium exposure were identified. The nucleotide sequences of the majority of the differentially expressed cDNAs are identical to those of C. elegans cosmids, yeast artificial chromosomes, expressed sequence tags, or predicted genes. The translated amino acid sequences of several clones are identical to C. elegans metallothionein-1, HSP70, collagens, and rRNAs. In addition, C. elegans homologues of pyruvate carboxylase, DNA gyrase, ␤-adrenergic receptor kinase, and human hypothetical protein KIAA0174 were identified. The translated amino acid sequences of the remaining differentially expressed cDNAs encode novel proteins.

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