Serum Intestinal Fatty Acid Binding Protein and Phosphate Levels in the Diagnosis of Acute Intestinal Ischemia: An Experimental Study in Rabbits

Dündar, Zerrin Defne; Cander, Başar; Gül, Mehmet; Karabulut, Keziban Uçar; Koçak, Sedat; Girisgin, Sadik; Mehmetoğlu, İdris; Toy, Hatice

doi:10.1016/j.jemermed.2011.05.051

Cited by 19 publications

(16 citation statements)

References 19 publications

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“…However, recent clinical studies have found elevations of iFABP during acute intestinal necrosis (35). Not all studies have been consistent: iFABP was not correlated to intestinal IR at any time point (1, 3 and 6 hours reperfusion) in a recent study in rabbits (36). Together with our results, this suggests that the role of iFABP as a biomarker for intestinal ischemia remains unclear.…”

Section: Discussionmentioning

confidence: 91%

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Garza¹,

Ibarra-Hernández²,

Cordero-Pérez³

et al. 2013

Clinics

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OBJECTIVE:It is essential to identify a serological marker of injury in order to study the pathophysiology of intestinal ischemia reperfusion. In this work, we studied the evolution of several serological markers after intestinal ischemia reperfusion injury in rats. The markers of non-specific cell damage were aspartate aminotransferase, alanine aminotransaminase, and lactic dehydrogenase, the markers of inflammation were tumor necrosis factor alpha, interleukin-6, and interleukin-1 beta, and the markers of intestinal mucosal damage were intestinal fatty acid binding protein and D-lactate. We used Chiús classification to grade the histopathological damage.METHODS:We studied 35 Wistar rats divided into groups according to reperfusion time. The superior mesenteric artery was clamped for 30 minutes, and blood and biopsies were collected at 1, 3, 6, 12, 24, and 48 hours after reperfusion. We plotted the mean ± standard deviation and compared the baseline and maximum values for each marker using Student's t-test.RESULTS:The maximum values of interleukin-1 beta and lactic dehydrogenase were present before the maximal histopathological damage. The maximum tumor necrosis factor alpha and D-lactate expressions coincided with histopathological damage. Alanine aminotransaminase and aspartate aminotransferase had a maximum expression level that increased following the histopathological damage. The maximum expressions of interluken-6 and intestinal fatty acid binding protein were not significantly different from the Sham treated group.CONCLUSION:For the evaluation of injury secondary to acute intestinal ischemia reperfusion with a 30 minute ischemia period, we recommend performing histopathological grading, quantification of D-lactate, which is synthesized by intestinal bacteria and is considered an indicator of mucosal injury, and quantification of tumor necrosis factor alpha as indicators of acute inflammation three hours after reperfusion.

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Section: Discussionmentioning

confidence: 91%

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Garza¹,

Ibarra-Hernández²,

Cordero-Pérez³

et al. 2013

Clinics

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“…[34] A meta-analysis determined that the sensitivity and specificity of the serum IFABP level in the diagnosis of intestinal ischemia was 72% (51-88%) and 73% (62-83%), respectively. [6] In the study conducted by Dundar et al, [35] no significant difference was detected between the serum IFABP level in control, sham, and ischemia groups. Furthermore, no significant difference was observed at the initial, first, third, and sixth hours of ischemia in the ischemia group.…”

Section: Ischemiamentioning

confidence: 85%

Role of Heparin Binding Protein (HBP) in the Diagnosis of Acute Mesenteric Ischemia

Koçak

Acar

Ertekin

et al. 2018

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Acute mesenteric ischemia (AMI) is associated with a high mortality rate, yet diagnostic difficulties persist. Although many biomarkers have been investigated for diagnostic purposes, as well as imaging methods, a sufficiently specific and sensitive marker has not been identified. This research was designed to examine whether heparin-binding protein (HBP), which has a role in the early phase of inflammation, could be useful in the diagnosis of AMI. METHODS: Serum samples obtained from a previously performed rabbit model of AMI were used in the study. HBP, C-reactive protein (CRP) and interleukin 6 (IL-6) levels were measured in blood samples obtained at baseline and 1, 3, and 6 hours from subjects that were separated into 3 groups: control, sham, and ischemia. The change in each marker over time and comparisons of the groups were evaluated statistically. RESULTS: A significant difference was not detected at the first hour in any of the studied markers. At the third hour, the CRP and IL-6 levels in the ischemia group indicated a significant increase in comparison with the control and sham groups (p<0.001). The HBP values showed a significant increase at the sixth hour in the ischemia group in comparison with the others (p<0.001). CONCLUSION: The HBP level demonstrated a slower increase in a rabbit model of AMI compared with CRP and IL-6. However, it still has the potential to become an early diagnostic biomarker. Diagnostic sensitivity and specificity should be evaluated in further clinical trials.

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“…По данным нескольких обзорных статей, фос-фор имеет низкую чувствительность, но высо-кую специфичность (26 и 82% соответственно). Однако сведений о клиническом использовании данного биомаркера не представлено [19,20].…”

Section: биохимические маркеры острой интестинальной ишемииunclassified

“…Однако в экспери-ментальном исследовании Z.D. Dundar разницы в уровне I-FABP в сыворотке крови кроликов с моделью острой интестинальной ишемии и в контрольной группе не получено [19]. Дан-ный биомаркер представляет большой интерес для дальнейшего исследования и внедрения в практику в силу его высокой чувствительности и тканевой специфичности.…”

Section: биохимические маркеры острой интестинальной ишемииunclassified

New Biomarkers of Acute Mesenteric Ischemia

Чернооков¹,

Bozhedomov²,

Atayan³

et al. 2018

Novosti Khirurgii

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СОВРЕМЕННЫЕ БИОМАРКЕРЫ ОСТРОЙ ИНТЕСТИНАЛЬНОЙ ИШЕМИИПервыйОстрая интестинальная ишемия является одной из наиболее сложных проблем в ургентной хирургии в силу высокой летальности, одной из причин которой является поздняя диагностика. Оперативное лече-ние часто проводится в фазу разлитого перитонита.С целью выделения наиболее доступных и точных способов ранней диагностики острой интести-нальной ишемии выполнен данный обзор литературы.В настоящее время для диагностики острой интестинальной ишемии используют немногочислен-ный ряд биомаркеров, таких как α-глутамат-S-трансфераза, D-димеры, прокальцитонин, D-лактат, кишеч-ный белок, связывающий жирные кислоты (I-FABP), ишемия-модифицированный альбумин. По данным литературы, наиболее высокие показатели чувствительности и специфичности выявлены у I-FABP (75-85% и 70-80% соответственно), α-глутамат-S-трансферазы (67,8% и 84,2%), ишемия-модифицированного альбумина (94,7% и 86,4%). Кроме этого, для ранней диагностики в настоящее время используются до-рогостоящие и инвазивные методы, такие как КТ-ангиография, МРТ с контрастированием, селективная ангиография, однако данные технологии доступны не всем лечебным учреждениям.Для решения проблемы улучшения ранней диагностики острой интестинальной ишемии следует про-водить дальнейший поиск различных биомаркеров и их более широкое внедрение в клиническую практику. Ключевые слова: острая интестинальная ишемия, биомаркеры, диагностика, мезентериальный тромбоз, кишечный белок, связывающий жирные кислоты (I-FABP)The acute mesenteric ischemia is one of the most complex problems in the urgent surgery because of the high mortality, the cause of which is late diagnosis. The operation treatment is often provided in the phase of diffuse peritonitis.This literature review is done in order to identify the most accessible and accurate methods of early diagnosis of the acute mesenteric ischemia.At present time rather a small number of biomarkers for diagnosing the acute mesenteric ischemia are used, such as α-glutamate-S-transferase, D-dimers, procalcitonin, D-lactate, intestinal fatty acid binding protein (I-FABP), ischemia-modified albumin. According to the literature the highest sensitivity and specificity were found in I-FABP (75-85% and 70-80% respectively), α-glutamate-S-transferase (67.8% and 84.2%), ischemia-modified albumin (94.7% and 86.4%). In addition, expensive and invasive methods are currently used for early diagnosis, such as CT angiography, contrast-enhanced MRI, selective angiography. However, these technologies are not available to all medical institutions.We should continue further search of various biomarkers and their more widespread introduction to clinical practice in order to solve the problem of early acute mesenteric ischemia diagnostics.

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Serum Intestinal Fatty Acid Binding Protein and Phosphate Levels in the Diagnosis of Acute Intestinal Ischemia: An Experimental Study in Rabbits

Cited by 19 publications

References 19 publications

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

Role of Heparin Binding Protein (HBP) in the Diagnosis of Acute Mesenteric Ischemia

New Biomarkers of Acute Mesenteric Ischemia

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