Serum-isolated exosomes from Piscirickettsia salmonis-infected Salmo salar specimens enclose bacterial DnaK, DnaJ and GrpE chaperones

Muñoz, Cristián; Carmona, Marisela; Luna, Omar; Gómez, Fernando; Cárdenas, Constanza; Flores-Herrera, Patricio; Belmonte, Rodrigo; Marshall, Sergio H.

doi:10.1016/j.ejbt.2022.07.003

Cited by 2 publications

(3 citation statements)

References 56 publications

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“…Reports describing EVs in other bony fish species is also limited (Zhao et al 2022 ), including Atlantic cod ( Gadus morhua L. ) (Lange et al 2019 ; Magnadóttir et al 2019 ; Magnadóttir et al 2020 ), Atlantic salmon ( Salmo salar ) (Iliev et al 2010 ; Lagos et al 2017 ; Iliev et al 2018 ; Smith et al 2020 , Leiva et al 2021 ; and Muñoz et al 2022 ) and Zebrafish ( Danio Rerio ) (Ohgo et al 2020 ; Scott et al 2021 ; Kobayashi-Sun et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Muñoz et al ( 2022 ) described plasma derived EVs from Piscirickettsia salmonis -infected Atlantic salmon using the combination of: 1,500 g 10 min, 10,000 g 10 min centrifuge steps with qEV size exclusion columns separating large EVs, exosomes and serum proteins and analyzed RNA and protein cargo of small EVs. EVs were isolated in a range of 60-250 nm with 6.62E + 8—7.08E + 8 particles/mL concentration measured by NTA, with spherical morphology at a size range of 50–125 nm by TEM, and using an EV marker MHC-II in western blot.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

Pelyhe,

Sturve

2023

Fish Physiol Biochem

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Extracellular vesicles (EVs) are 50–1,000 nm lipid bilayer-bound vesicles, released into the extracellular environment by various cell types for intercellular communication purposes. The quantitative and qualitative characteristics of EVs can be affected by stress and pathological conditions. The majority of extracellular vesicle (EV) studies have been performed on mammalian cell lines or bodily fluids. EVs have been previously described from bodily fluids like plasma, serum or mucus in different fish species, however the available knowledge of fish cell line derived EVs is limited and in the vast majority of studies, the overall focus is on small EVs (< 200 nm). We isolated large and small extracellular vesicles from zebrafish (Danio rerio) liver (ZFL), rainbow trout (Oncorhynchus mykiss) liver (RTL-W1), gill (RTgill-W1) and intestinal epithelial (RTgutGC) cell lines using stepwise centrifugation and characterized the size and morphology of EVs. Here we demonstrated that large and small extracellular vesicles can be successfully isolated using stepwise centrifugation from the serum-free medium of the selected piscine cell lines after a 24-h incubation period. The size distribution of large and small EVs isolated from the piscine cell lines suggest that large and small EV groups show high diversity in size ranges, containing heterogenous subpopulations in sizes, and the results highly depend on the applied method and whether filtration steps were included following the isolation. The spherical morphology of EVs was verified by transmission electron microscopy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

Pelyhe,

Sturve

2023

Fish Physiol Biochem

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“…Cell-to-cell communication [1] involves the transmission of a signal from a sending cell to a receiving cell, and this process may be reciprocal. Cell-to-cell communication involves life processes and can even induce cell death [2]. Exosomes are a subset of extracellular vesicles (EVs) of endosomal origin and in a size range of ~40 to 160 nm in diameter (~100 nm on average), containing nucleic acids, proteins, lipids, amino acids, and metabolites [3].…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Experimental Method for Studying Cell-to-Cell Exosome Delivery–Taking Stem Cell–Tumor Cell Interaction as a Case

Peng,

Su,

Guo

et al. 2023

IJMS

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Cell-to-cell communication must occur through molecular transport in the intercellular fluid space. Nanoparticles, such as exosomes, diffuse or move more slowly in fluids than small molecules. To find a microfluidic technology for real-time exosome experiments on intercellular communication between living cells, we use the microfluidic culture dish’s quaternary ultra-slow microcirculation flow field to accumulate nanoparticles in a specific area. Taking stem cell–tumor cell interaction as an example, the ultra-slow microcirculatory flow field controls stem cell exosomes to interfere with tumor cells remotely. Under static coculture conditions (without microfluidics), the tumor cells near stem cells (<200 µm) show quick breaking through from its Matrigel drop to meet stem cells, but this ‘breaking through’ quickly disappears with increasing distance. In programmed ultra-slow microcirculation, stem cells induce tumor cells 5000 μm far at the site of exosome deposition (according to nanoparticle simulations). After 14 days of programmed coculture, the glomeration and migration of tumor cells were observed in the exosome deposition area. This example shows that the ultra-slow microcirculation of the microfluidic culture dish has good prospects in quantitative experiments to study exosome communication between living cells and drug development of cancer metastasis.

show abstract

Serum-isolated exosomes from Piscirickettsia salmonis-infected Salmo salar specimens enclose bacterial DnaK, DnaJ and GrpE chaperones

Cited by 2 publications

References 56 publications

Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

Isolation and characterization of the morphology, size and particle number of rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio) cell line derived large and small extracellular vesicles

A Microfluidic Experimental Method for Studying Cell-to-Cell Exosome Delivery–Taking Stem Cell–Tumor Cell Interaction as a Case

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