Serum Lactate Dehydrogenase Activity and Visceral Organs and Body Weights of Diabetic Rats Administered Single and Combinatorial Herbal Formulations

Chikezie, Chiwendu Maureen; Ojiako, Okey A.; Emejulu, A. A.; Chikezie, Paul Chidoka

doi:10.5530/pc.2018.1.7

Cited by 4 publications

(10 citation statements)

References 36 publications

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“…The leaves were dried to constant weight at 50 • C for 10-12 h 32 . The dried leaf samples were pulverized and subsequently stored for use as previously described 33 .…”

Section: Preparation Of Leaf Samplesmentioning

confidence: 99%

“…The male Wistar rats, within the ages of 7 -9 weeks old and of average weight of 109.74 ± 2.81 g, were obtained from the Animal House of Imo State University, Owerri, Nigeria. Handling of the animals was performed according to the methods previously described 33 . The Ethical Committee on the use of animals for research, Department of Biochemistry, Imo State University, Owerri, Nigeria (Ethics Approval Number: ODVC/REN/1232/19) approved the present study.…”

Section: Animal Handling and Experimental Designmentioning

confidence: 99%

“…The serum was measured for hepatorenal tissues biomarkers. The collection and preparation of the liver and kidneys, in order to ascertain their respective weights, were done according to the methods previously described 33 .…”

Section: Collection and Preparation Of Blood Liver And Kidneysmentioning

confidence: 99%

“…The body weight of the rats was measured using electronic weighing balance {Digital Precision Weighing Balance (JCS-QC03) -China}, on the 1 st and 21 st days of the experiment 33…”

Section: Body Weight Of Ratsmentioning

confidence: 99%

“…The liver and right and left kidneys weights were measured on day 21. The organ weight and body weight were reported in grams 33 .Thus:…”

Section: Liver and Kidney Weights To Body Weight Ratiosmentioning

confidence: 99%

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Acute dysfunctional status of hepatorenal tissues of rats administered with leaf extracts of Ocimum gratissimum L. (Lamiaceae)

et al. 2020

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Background: Given the vast medicinal properties of Ocimum gratissimum, the present study evaluated, in comparative terms, the acute dysfunctional status of hepatorenal tissues of Wistar rats administered with petroleum ether (PE) and ethyl acetate (EA) leaf extracts of O. gratissimum. Methods: Grouping of the experimental rats was assigned according to the treatments given, in which graded doses {200, 400, 600 and 800 mg/kg body weight (b.w.)} of PE and EA fractions of O. gratissimum leaf extract were administered to the rats by oral gavage on a daily basis for a period of 21 days. Serum levels of hepatorenal tissues biomarkers were measured using standard spectrophotometric methods. The organ-to-body weight ratio of the rats was measured on the 21st day of the experiment. Results: Serum aspartate aminotransferase (AST) to alanine aminotransferase (ALT) ratio (i.e. AST/ALT) of the experimental rat groups was found to be within the range of 0.919 – 1.022 unit. The experimental rat groups administered with PE and EA fractions of O. gratissimum leaf extract showed dose-dependent increasing levels of serum alkaline phosphatase (ALP) activity. Likewise, rat groups administered with the herbal extracts exhibited increasing serum total bilirubin, urea and creatinine concentrations, in a dose-dependent manner. At the end of the 21-day treatment period, all the experimental rat groups showed increase in body weight, ranging from 0.79 – 1.98% increase. The liver weight and kidney weight to body weight ratios were within the range of (0.0468 0.02 – 0.0981 0.04) unit and (0.00245 0.002 – 0.01968 0.007) unit, respectively. Conclusion: The results showed that doses of PE and EA fractions of O. gratissimum leaf extract greater than 400 mg/kg b.w. induced dose-dependent hepatorenal toxicity, with the EA fraction provoking greater toxicity than the PE fraction of O. gratissimum leaf extract.

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“…The leaves were dried to constant weight at 50 • C for 10-12 h 32 . The dried leaf samples were pulverized and subsequently stored for use as previously described 33 .…”

Section: Preparation Of Leaf Samplesmentioning

confidence: 99%

Section: Animal Handling and Experimental Designmentioning

confidence: 99%

Section: Collection and Preparation Of Blood Liver And Kidneysmentioning

confidence: 99%

“…The body weight of the rats was measured using electronic weighing balance {Digital Precision Weighing Balance (JCS-QC03) -China}, on the 1 st and 21 st days of the experiment 33…”

Section: Body Weight Of Ratsmentioning

confidence: 99%

“…The liver and right and left kidneys weights were measured on day 21. The organ weight and body weight were reported in grams 33 .Thus:…”

Section: Liver and Kidney Weights To Body Weight Ratiosmentioning

confidence: 99%

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Acute dysfunctional status of hepatorenal tissues of rats administered with leaf extracts of Ocimum gratissimum L. (Lamiaceae)

et al. 2020

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Protective Effect of Tertiary Butylhydroquinone against Obesity-induced Skeletal Muscle Pathology in Post-weaning High Fat Diet Fed Rats

Zhang

2024

CPB

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Background: Obesity deleteriously affects skeletal muscle functionality starting from infancy to adulthood, leading to dysfunctional skeletal muscle. Objectives: This study, therefore, evaluated the protective action of tert-butylhydroquinone (tBHQ) against obesity-induced skeletal muscle pathology in high-fat diet (HFD) fed rats. Methods: Twenty post-weaning male albino rats were randomized into four groups of five rats each as: Group 1 (control), Group 2 (HFD), Group 3 (orlistat) and Group 4 (tBHQ). Group one received rat pellets for 12 weeks, while groups 2 to 4 received HFD for 12 weeks. At the end of week 8, obesity was confirmed with Lee Obesity Index and body mass index values of ≥ 303 and ≥ 0.68 gcm2, respectively. Group 3 was given oral administration of orlistat (10 mg/kg, once daily), while group 4 was given oral administration of tBHQ (25 mg/kg, once daily). Administration of orlistat and tBHQ commenced from week 9 to the end of the experiment. Results: Chronic exposure of post-weaning rats to HFD led to their development of the metabolic syndrome phenotypes in adulthood, characterized by obesity, hyperglycemia, dyslipidaemia, hyperinsulinaemia, insulin resistance as well as induction of oxidative stress and alteration of skeletal muscle markers, which were mitigated following supplementation with orlistat and tBHQ. Conclusion: The study showed the anti-obesity potentials of tBHQ and its protective action against HFD obesity-induced skeletal muscular pathology.

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Alterations of Obestatin, Cardiac Markers and Lipid Profile Levels in Type 2 Diabetes Mellitus

Balaky¹,

Kakey

2021

eijs

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The current investigation aimed to test the alterations of the levels of obestatin hormone, lipid profile and cardiac function markers in relation to hyperglycemia in patients with non-insulin diabetes mellitus. The study included 118 diabetic subjects (56 males, 62 females) and 60 healthy non-diabetic subjects (30 males, 30 females). Diabetic and healthy subjects were age-matched. Serum levels of obestatin, lipid profile markers including total cholesterol (STC), triglycerides (STG), low and high density lipoproteins (LDL-C and HDL-C), as well as cardiac function markers including, creatine kinase and lactate dehydrogenase enzymes were determined in all subjects. The findings revealed a remarkable decrease in the level of serum obestatin in both diabetic males and females with both age ranges (40-59 and 60-80 years). On the other hand, the results showed that serum STC, LDL-C, and STG levels were statistically significantly elevated, while that for HDL-C was significantly decreased in diabetic males and females with both age ranges. Concerning the cardiac markers, the results found out that the levels of CK-MB and LDH were significantly increased in type 2 diabetic males and females with both age ranges. The results suggested that a low concentration of obestatin is a significant risk factor for type 2 DM, with a key role for this hormone in the pathogenesis. Accordingly, altered levels of obestatin could be used as an important indicator for type 2 DM. The current study also suggests a direct relationship between lipid profiles, except for HDL, the decreased obestatin level, the increased cardiac function markers, and hyperglycemic status in type 2 DM.

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Serum Lactate Dehydrogenase Activity and Visceral Organs and Body Weights of Diabetic Rats Administered Single and Combinatorial Herbal Formulations

Cited by 4 publications

References 36 publications

Acute dysfunctional status of hepatorenal tissues of rats administered with leaf extracts of Ocimum gratissimum L. (Lamiaceae)

Acute dysfunctional status of hepatorenal tissues of rats administered with leaf extracts of Ocimum gratissimum L. (Lamiaceae)

Protective Effect of Tertiary Butylhydroquinone against Obesity-induced Skeletal Muscle Pathology in Post-weaning High Fat Diet Fed Rats

Alterations of Obestatin, Cardiac Markers and Lipid Profile Levels in Type 2 Diabetes Mellitus

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