Serum lactate dehydrogenase isoenzyme activities in patients with asthma

Usher, D. J.; Shepherd, Robert J.; Deegan, T.

doi:10.1136/thx.29.6.685

Cited by 7 publications

(8 citation statements)

References 18 publications

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“…Moreover, we also detected significant differential expression between the two responder groups in LDHC ( Fig 4B ). The level of lactate dehydrogenase level in sputum has been established as a diagnostic marker for asthma [ 28 , 29 ], and our data suggest that it might also be a useful marker for steroid treatment response. Among all verified sites, no CpG sites were found to be strongly associated with poorly controlled asthma (ACT score), or base line asthma symptom scores.…”

Section: Discussionmentioning

confidence: 57%

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Nasal DNA methylation differentiates corticosteroid treatment response in pediatric asthma: A pilot study

et al. 2017

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BackgroundTreatment response to systemic corticosteroid in asthmatic children is heterogeneous and may be mediated by epigenetic mechanism(s). We aim to identify DNA methylation (DNAm) changes responsive to steroid, and DNAm biomarkers that distinguish treatment response.Materials and methodsWe followed 33 children (ages 5–18) presenting to the Emergency Department (ED) for asthma exacerbation. Based on whether they met discharge criteria in ≤24 hours, participants were grouped into good and poor responders to steroid treatment. Nasal samples were collected upon presentation to the ED (T0) and 18–24 hours later (T1). Genome-wide DNAm was measured for both time points in 20 subjects, and compared between T0 and T1 in good and poor responders respectively. DNAm at T1 was also compared between two responder groups. DNAm of selected CpGs was verified in the complete cohort, and expression of associated genes was examined. Interactions between DNAm, common single nucleotide polymorphism (SNP) located at the CpG sites and treatment responses were assessed.ResultsThree CpGs located in the OTX2 promoter showed responder-specific DNAm changes from T0 to T1, in which DNAm decreased in good but not in poor responders. Good and poor responders showed differential DNAm at T1 in 127 CpGs without and 182 CpGs with common SNP co-localization. Negative correlations between DNAm and gene expression were observed at CpGs located within the LDHC promoter, suggesting an impact of DNAm on gene regulation. Interactions between SNPs, DNAm and treatment response were detected.ConclusionAcute systemic steroid treatment modifies nasal DNAm in good responders. Nasal DNAm, dependent or independent of SNPs, can differentiate response to treatment in acute asthmatic children.

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Section: Discussionmentioning

confidence: 57%

“…LDHC encodes lactate dehydrogenase and is highly expressed in asthmatics. Its level in sputum has been established as a diagnostic marker for asthma [ 28 , 29 ]. DNHD1 encodes Dynein Heavy Chain Domain 1, which is a protein complex that is involved in microtubule movement and expressed in the lung.…”

Section: Resultsmentioning

confidence: 99%

Nasal DNA methylation differentiates corticosteroid treatment response in pediatric asthma: A pilot study

et al. 2017

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“…In bronchial asthma, the rise is predominantly in LDH‐3 and LDH‐5. In the same study, the predominant isoenzymes in lung tissue were found to be LDH‐3 and LDH‐4 (2).…”

Section: Discussionmentioning

confidence: 70%

“…This observation has not been widely reported and in the available studies, specific isoenzymes of LDH have often not been analysed. Raised LDH values have been observed in immunologically mediated lung injury, lung infections, obstructive airways disease, parenchymal lung diseases as well as pulmonary vascular disease (2–13, 22, 23). In respiratory disease, isoenzymes 3, 4 and 5 are increased as compared to the normal serum pattern.…”

Section: Discussionmentioning

confidence: 99%

“…Several respiratory disorders have been reported to be associated with raised serum LDH levels. These include obstructive airways disease such as bronchial asthma and chronic obstructive pulmonary disease (COPD) (2–4). Raised LDH levels have been noted in respiratory infections such as pulmonary tuberculosis, bronchopneumonia, Pneumocystis jiroveci pneumonia, avian influenza and the corona virus‐related severe acute respiratory syndrome (5–10).…”

Section: Introductionmentioning

confidence: 99%

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Serum LDH in chronic cough: a potential marker of airway inflammation

Faruqi

Wilmot

Wright

et al. 2011

Clinical Respiratory J

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Introduction and Objectives: Lactate dehydrogenase (LDH) is found in almost all tissues of the body and five different isoenzymes are known (LDH-1 to LDH-5). LDH can be elevated in many pathological conditions. We have observed serum LDH to be increased in patients with chronic cough. We wanted to confirm this finding, study the reproducibility and determine the origin of the LDH. Methods: Patients prospectively seen at the Hull Cough Clinic had total and specific LDH isoenzyme levels in serum determined. A subgroup of patients also had a serum creatine phosphokinase (CK) measured. Patients completed cough symptom scores and the Hull Airway Reflux Questionnaire (HARQ). Spirometry was performed. Results: Eighty-three patients were included. Forty-two percent had LDH values above the reference range and 78% had LDH values in the fourth quartile of the reference range or above. This increase in LDH was predominantly because of a rise in isoenzymes 4 and 5. The increase in LDH was found to be reproducible at 8 weeks. Ten percent had CK values above the normal range. There was no correlation observed between LDH values and the cough scores, HARQ scores or lung function. Conclusion: Serum LDH levels are elevated in a substantial proportion of patients with chronic cough. This rise is likely to be due to airway inflammation known to be associated with chronic cough.Please cite this paper as: Faruqi S, Wilmot R, Wright C and Morice AH. Serum LDH in chronic cough: a potential marker of airway inflammation. Clin Respir J 2012; 6: 81-87.

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Clinical biochemistry of lactate dehydrogenase

Skillen¹

1984

Cell Biochemistry & Function

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Serum lactate dehydrogenase isoenzyme activities in patients with asthma

Cited by 7 publications

References 18 publications

Nasal DNA methylation differentiates corticosteroid treatment response in pediatric asthma: A pilot study

Nasal DNA methylation differentiates corticosteroid treatment response in pediatric asthma: A pilot study

Serum LDH in chronic cough: a potential marker of airway inflammation

Clinical biochemistry of lactate dehydrogenase

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