Serum Level and Gene Expression of Interleukin-15 Do Not Correlate with Villous Atrophy in Celiac Disease Patients

Aghamohamadi, Elham; Kokhaei, Parviz; Rostami-Nejad, Mohammad; Pak, Fatemeh; Rostami, Kamran; Moradi, Abdolvahab; Pourhoseingholi, Mohamad Amin; Chaleshi, Vahid; Masotti, Andrea; Zali, Mohammad Reza

doi:10.1089/gtmb.2019.0265

Cited by 2 publications

(2 citation statements)

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“…Like many autoimmune diseases, several cytokines and signaling proteins play roles in CD pathogenesis. 31 According to our results, the expression level of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 genes in the peripheral blood sample of the patients with CD was significantly increased compared with the control group.…”

Section: Discussionsupporting

confidence: 51%

“…5 Aghamohamadi and colleagues demonstrated that IL-15 gene expression is increased in biopsy specimens of CD patients with Marsh II compared with the control group. 31 Mention and co-workers also found the overexpression of IL-15 in lamina propria and intestinal epithelium of patients with active CD. 9 TBX21/T-bet is a transcription factor induced by IL-15 in the intestine of patients with CD that causes IFN-γ production.…”

Section: Discussionmentioning

confidence: 91%

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Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases

Khalkhal

Rezaei‐Tavirani

Asri

et al. 2022

Middle East J Dig Dis

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BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of interleukin-15 (IL-15), interleukin-17A (IL-17A), interleukin23A (IL-23A), granzyme B (GzmB), T-box transcription factor 21 (TBX21), and tumor necrosis factor alpha-induced protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group. METHODS: Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic (ROC) curve analysis was performed to characterize the diagnostic ability of the studied genes. RESULTS: The expression of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GzmB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls. CONCLUSION: Our results suggest that TNFAIP3, IL23A, and GzmB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated.

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Section: Discussionsupporting

confidence: 51%