Serum Levels of Androstenedione, Testosterone and Dehydroepiandrosterone Sulfate in Patients with Premature Ovarian Failure to Age-Matched Menstruating Controls

Elias, Alan N.; Pandian, M. R.; Rojas, Fausto

doi:10.1159/000291817

Cited by 28 publications

(25 citation statements)

References 7 publications

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“…In this group, testosterone and DHEA concentrations were similar as female controls, while AD concentrations were significantly lower. In the existing literature, it is debated whether POI is associated with decreased androgen concentrations (14,(29)(30)(31)(32)(33), and only one study presented with results similar to ours (34). As previous studies only applied direct RIA, chemiluminescence immunoassays, and extraction RIA, conflicting findings most probably resulted from lower sensitivity, lack of specificity, or lower accuracy of these assays (10).…”

Section: Discussionmentioning

confidence: 48%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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Objective: The measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over-or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatographytandem mass spectrometry (LC-MS/MS) in these women. Design: Cross-sectional study. Methods: Carefully phenotyped women with POI (nZ208) or PCOS (nZ200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC-MS/MS and extraction RIA. Results: All androgen levels were significantly elevated in women with PCOS compared with POI patients (P!0.05) and controls (P!0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC-MS/MS were systematically lower. However, using extraction RIA and LC-MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland-Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0. 94-0.91), 0.83 (0.79-0.86), and 0.96 (0.95-0.97) respectively. Conclusions: LC-MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC-MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required. European Journal of Endocrinology 165 925-933

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Section: Discussionmentioning

confidence: 48%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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“…Women with POI have significantly lower levels of free and total testosterone than regular menstruating women of similar age; this phenomenon is even more apparent following surgical menopause. 4,42,47,48 Improved sexual function and overall sense of well-being are described with the addition of androgen to estrogen-based hormonal regimens. 49 Although data are limited, androgen replacement may be of consideration in the certain women who remain symptomatic despite use of estrogen-based hormone therapy of adequate dose and duration.…”

Section: Sexuality: Role For Androgen Therapy?mentioning

confidence: 99%

Premature Menopause

Torrealday

Pal

2015

Endocrinology and Metabolism Clinics of North America

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A heterogeneous disorder, premature menopause is not an uncommon entity, affecting approximately 1% of women younger than 40 years. Multisystem implications are recognized as sequelae to the premature deprivation of ovarian steroids, posing unique health-related challenges in this population. An integrated management approach that addresses both the physical and psychological health concerns and the overall well-being of this relatively chronologically young population is paramount.

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“…Also there might be loss of ovarian androgens because of atrophy of the ovarian cortex (Elias, Pandian & Rojas, 1997). The primary basis of treatment used is estroprogestative hormone therapy, recommended at least until the average age of natural menopause, to reduce or control impacts related to hypoestrogenism (Maclaran & Panay, 2011).…”

Section: Introductionmentioning

confidence: 99%

The Role of Premature Ovarian Failure Awareness in Female Sexual Functions and Distress

Aydın

Ates

Aydın

et al. 2016

Journal of Sex & Marital Therapy

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The diagnosis of premature ovarian failure is traumatic to women, with loss of gonadal functions having been associated with distress and anxiety. The aim of this study is to evaluate the sexual function and distress of women with premature ovarian failure before the diagnosis. Women with premature ovarian failure and age-matched controls were evaluated through the Female Sexual Function Index and the Female Sexual Distress Scale-Revised, and their androgen levels were compared. The major finding of this study is the lack of difference between sexual function in women who are unaware that they have premature ovarian failure and age-matched women with normal gonadal function.

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Serum Levels of Androstenedione, Testosterone and Dehydroepiandrosterone Sulfate in Patients with Premature Ovarian Failure to Age-Matched Menstruating Controls

Cited by 28 publications

References 7 publications

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Premature Menopause

The Role of Premature Ovarian Failure Awareness in Female Sexual Functions and Distress

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