Serum levels of Antioxidant in patients with Oral Squamous Cell Carcinoma: a preliminary study

Bagul, Neeta; Ganjre, Anjali P; Kheur, Supriya; Patekar, Deepali; Dasgupta, Subrata; Mahalle, Aditi

doi:10.9790/0853-1152832

Cited by 3 publications

(6 citation statements)

References 16 publications

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“…Opposite results were obtained by Bagul et al [37]. They demonstrated statistically significant increase in GPx and SOD activities in OSCC patients compared with controls.…”

Section: Discussionmentioning

confidence: 65%

Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal Cancer Associated with EBV Infection

Strycharz-Dudziak

Kiełczykowska

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et al. 2019

Oxidative Medicine and Cellular Longevity

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A growing number of studies reveal that oxidative stress is associated with viral infections or cancer development. However, there are few reports assessing the relationships between oxidative stress, viral infection, and carcinogenesis. The present study analyzed the level of total antioxidant status (TAS) as well as the activities of glutathione peroxidase (GPx) and superoxide dismutase (SOD) in patients with oropharyngeal cancer both Epstein-Barr virus (EBV)-positive and EBV-negative in comparison with the control group. The correlations between these parameters and EBV type (wild-type LMP1 (wt-LMP1) or LMP1 with deletion (del-LMP1)), level of antibodies against EBV, the degree of tumor differentiation, and TNM classification were also investigated. Fresh frozen tumor tissue samples collected from 66 patients with oropharyngeal squamous cell carcinoma were tested using nested PCR assay for EBV DNA detection. Spectrophotometric methods were used to measure TAS values as well as SOD and GPx activities in homogenates of tissue, using diagnostic kits produced by Randox Laboratories. Sera from all individuals were investigated using ELISA method to detect the presence of Epstein-Barr virus capsid antigen (EBVCA) IgM and IgG, Epstein-Barr virus nuclear antigen (EBNA) IgG, and early antigen (EA) IgG antibodies. The level of TAS and activities of antioxidant enzymes (GPx and SOD) were significantly decreased in tissues with oropharyngeal cancer, particularly in EBV-positive cases. In 82.3% of patients, wt-LMP1 was detected. Significantly lower TAS, GPx, and SOD values were stated in patients infected with wild-type EBV. The presence of antibodies against early antigen (anti-EA) was detected in over 80% of patients, which suggests reactivation of EBV infection. The correlation between the degree of tumor differentiation and TN classification, especially in EBV-positive patients, was also observed. Determination of these parameters may be useful in evaluating tumor burden in patients with various stages of oropharyngeal cancer and could be an important prognostic factor. Future studies are needed to understand the role of EBV lytic reactivation induced by oxidative stress.

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“…Opposite results were obtained by Bagul et al [37]. They demonstrated statistically significant increase in GPx and SOD activities in OSCC patients compared with controls.…”

Section: Discussionmentioning

confidence: 65%

Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal Cancer Associated with EBV Infection

Strycharz-Dudziak

Kiełczykowska

Drop

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Out of the four included studies that analyzed the GPx activity in blood samples except one, 48 the remaining studies displayed diminished enzyme activity in the OSCC group compared to healthy controls ( p < .05) 26,30,35 . In consideration of serum samples, three studies displayed reduced activity, 25,37,51 and the remaining two depicted enhanced GPx activity in the cancerous group compared to the normal control group ( p < .05) 34,45 . When considering GPx values in salivary samples, two studies displayed intensified activity ( p < .05) 39,49 ; however, one research depicted an insignificant reduction of the GPx activity 50 .…”

Section: Discussionmentioning

confidence: 94%

“…26,30,35 In consideration of serum samples, three studies displayed reduced activity, 25,37,51 and the remaining two depicted enhanced GPx activity in the cancerous group compared to the normal control group (p < .05). 34,45 When considering GPx values in salivary samples, two studies displayed intensified activity (p < .05) 39,49 ; however, one research depicted an insignificant reduction of the GPx activity. 50 The lympholysate sample described declined GPx enzyme activity in the cancerous group compared to the normal control group (p < .05).…”

Section: Discussionmentioning

confidence: 99%

Assessment of glutathione peroxidase enzyme response and total antioxidant status in oral cancer – Systematic review and meta‐analysis

et al. 2023

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BackgroundOxidative stress induced by free radical accumulation contributes to many pathologies, including cancer. Antioxidant defense system fails to scavenge free radicals when it is excessively accumulated. Assessing individual antioxidant enzymes and total antioxidant capacity could direct the customized therapeutic strategies.ObjectiveEvaluation of total antioxidant status and enzyme glutathione peroxidase activity in the oral cancer group compared to the healthy control group.MethodThe literature search included databases PubMed, Science Direct, Wiley Online Library, Cochrane and Cross Reference between 1999 and 2021. The database search was completed in the month of August 2022. The extracted data were analyzed by Comprehensive Meta‐Analysis (CMA) version 3 software (Biostat Inc. Englewood, NJ). Based on search strategies, 1435 articles have been retrieved from the database. In the segregated articles, 1365 were excluded due to duplicated articles, animal studies, low‐quality studies, articles unrelated to the research question, and with unmatched objectives. Based on inclusion criteria, 70 articles were selected for full‐text valuation. However, 33 articles were found highly suitable for inclusion and data extraction. Finally, 11 articles were selected for meta‐analysis.ResultsThe meta‐analysis of four included studies of tissue samples showed a significantly (p < .001) increased GPx activity in the oral cancer group, when compared to the control group, whereas three included studies of erythrocyte samples displayed a significantly (p < .001) decreased GPx activity in the oral cancer group than the control group with the pooled standardized mean difference value of −2.766 moles/min/g Hb at 95% CI (−3.297 to −2.234). The meta‐analysis of the included studies depicted an insignificant (p = .947) reduction of salivary TAS levels in the oral cancer group when compared to the control group.ConclusionOur systematic review and meta‐analysis depict antioxidant GPx enzyme activity in the regional tissue samples of the oral cancer group differs from other systemic biological fluid samples compared to the healthy control group.

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“…Similarly, two additional studies in saliva and blood documented a significant rise in SOD levels (P <0:05) within the OSCC group compared to the control group. [29,34,36,52] Another study revealed a statistically insignificant increase in SOD levels in erythrocytes of the OSCC group compared to the systemically healthy group (P >0:05) [28].…”

Section: Discussionmentioning

confidence: 95%

“…There could be a heightened dismutation of superoxide (O 2 − ) to H 2 O 2 with increased SOD activity. Other antioxidant enzymes detoxify the surplus H 2 O 2 in the blood cells [34,51]. Studies also indicated that as the activity of other The amount of enzyme necessary to inhibit 50% epinephrine autoxidation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Antioxidant Enzyme Superoxide Dismutase (SOD) in Oral Cancer: Systematic Review and Meta-Analysis

Mohideen,

Chandrasekaran,

et al. 2024

Disease Markers

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Objective. The present article aims to comprehensively review the existing literature on superoxide dismutase (SOD) levels, an antioxidant enzyme, in oral cancer. Method. An extensive literature search was conducted across various databases, including PubMed, Wiley Online Library, Science Direct, and Cross Reference, spanning 1998–2023. At the outset, 1,177 articles were initially identified, and 907 studies were excluded due to irrelevance or duplication of the research question. Subsequently, 270 articles underwent screening evaluation, resulting in the selection of 85 articles meeting the inclusion criteria. Following this, 68 articles underwent a full-text comprehensive assessment, and ultimately, 39 were chosen for data extraction. The risk of bias in the designated articles was assessed using the Newcastle–Ottawa Scale. Finally, 13 studies were meticulously selected, offering consistent data for the ensuing meta-analysis. Meta-analysis was executed using comprehensive meta-analysis (CMA) version 3 software (Bio Stat Inc., Englewood, NJ, USA). The meta-analysis findings revealed a statistically significant decrease in SOD levels in both erythrocyte samples (P<0.001) and tissue samples (P<0.05) among individuals with oral cancer (OSCC) compared to the normal control group. Conversely, the analysis of three studies on salivary samples demonstrated a significant increase (P<0.05) in SOD levels in the oral cancer group compared to the healthy controls. Conclusion. This systematic review underscores a statistically significant decline in SOD levels observed across diverse bio-samples in individuals with oral cancer, indicating an excess of oxidative stress (OS). Additional research is needed to delve into the relationship between SOD levels and clinic–pathological prognostic markers within the oral cancer cohort. Such investigations have the potential to significantly contribute to the development of prognostic tools grounded in OS, thereby guiding strategies for treatment planning.

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Serum levels of Antioxidant in patients with Oral Squamous Cell Carcinoma: a preliminary study

Cited by 3 publications

References 16 publications

Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal Cancer Associated with EBV Infection

Total Antioxidant Status (TAS), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) in Oropharyngeal Cancer Associated with EBV Infection

Assessment of glutathione peroxidase enzyme response and total antioxidant status in oral cancer – Systematic review and meta‐analysis

Assessment of Antioxidant Enzyme Superoxide Dismutase (SOD) in Oral Cancer: Systematic Review and Meta-Analysis

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