Serum levels of cytoskeleton remodeling proteins and their mRNA expression in tumor tissue of metastatic laryngeal and hypopharyngeal cancers

Abstract

Actin-binding proteins (ABPs) and various signaling systems are involved in the process of squamous cell carcinoma of the larynx and hypopharynx (SCCLH) metastasis. The clinical significance of these proteins has not yet been determined. We analyzed the relationship between the mRNA levels of cofilin 1 (CFL1), profilin 1 (PFN1), adenylyl cyclase-associated protein 1 (CAP1), SNAI1 and RND3 and SCCLH metastasis. The serum levels of the above ABPs were estimated and the relationship between them and their mRNA expressions was analyzed. The expression levels of ABP mRNAs were measured by real-time RT-PCR in paired tissue samples taken from 54 patients with SCCLH (T1-4N0-1M0). Expression analysis was performed using the 2 −ΔΔCT method. The levels of ABPs in the blood serum were measured by ELISA. Statistical analysis was carried out using the SPSS Statistica 20.0 software package. No significant difference in the mRNA gene expression in tumor tissue of patients with T1-3N0M0 SCCLH and patients with T2-4N1-2M0 SCCLH was found. High expression of RND3 mRNA was accompanied by an increase in mRNA expression of all studied ABPs. In the blood serum of T2-4N1-2M0 patients, the level of PFN1 was lower by 21% and the level of CAP1 was higher by 75% than those observed in T1-4N0M0 patients. The data obtained showed that RND3 is involved in the regulation of molecular cascades of SCCLH metastasis. PFN1 and CAP1 serum levels can be good classifiers of metastases in patients with SCCLH.

Epigenetic Regulation Mechanisms of the Cofilin-1 Gene in the Development and Differentiation of Bovine Primary Myoblasts

“…The spleen is the largest immune organ, and the data indicated that the CFL1 gene may be involved in mammalian cellular immunity and affect the development of tumors. This result is consistent with a previous report that the CFL1 gene can participate in cancer development [21,22]. However, the expression level of the CFL1 gene in adipose tissues was almost unchanged between calf and adult cattle, and the results suggested that the CFL1 gene was not involved in the regulatory mechanism of growth and differentiation during cattle adipose tissue development.…”

A pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients

“…There is also evidence of the involvement of CAP1 in signaling pathways [ 9 , 11 – 12 ] and caveolin-dependent endocytosis, in particular, in the internalization of low-density lipoprotein receptor (LDLR) and low density lipoprotein receptor-related protein (LRP) [ 13 – 14 ] . Recently, there have also been a number of studies suggesting that CFL1 and CAP1 work in conjunction to influence the aggressiveness of tumor development [ 10 , 12 , 14 – 15 ] .…”

The level of fascin-1 in circulating tumor cells and in cells of the local tumor site in patients with head and neck squamous cell carcinoma

“…We have previously shown that the genes encoding fascin (FSCN1), ezrin (EZR), and adenylyl cyclase associated protein 1 (CAP1) are involved in the development of HNSCC [14]. The prognostic signi cance of determining the content of pro lin 1 (PFN1) and CAP1 in the blood serum in relation to the development of lymph node metastases was shown [15]. FSCN1 was associated with cancer development in patients with premalignant laryngeal and hypolaryngeal lesions [14].…”