Serum levels of insulin-like growth factor (IGF) and its carrier protein in various metabolic disorders

Abstract

Abstract. The levels of insulin-like growth factors (IGF), two somatomedin-like polypeptides of human serum and of their carrier protein were determined in sera of patients with various metabolic disorders. IGF was measured by 4 different methods (fat pad and fat cell assay and competitive protein binding assay measuring total IGF, and a radioimmunoassay for IGF I) after extraction by acidic gel filtration on Sephadex G-50. This procedure is necessary to separate IGF from the carrier protein, which interferes with all of these assays. 1) In normal serum, immunoreactive IGF I accounts for one third of total IGF determined by the fat pad assay, but only for one fifth to one sixth of immunoreactive IGF I + II. 2) In acromegalics total IGF was increased 1.5- (protein binding and fat cell assay) to 2-fold (fat pad assay), but the increase was solely due to immunoreactive IGF I, which was ∼ 5-times above normal. The IGF binding activity was not elevated. Total IGF and IGF binding were decreased in hypopituitarism, Laron-type dwarfism and in liver cirrhosis. Immunoreactive IGF I was more drastically reduced in these diseases than total IGF. Apparently, only IGF I is under growth hormone control. The liver seems to be involved in the production of IGF. 3) No elevation of total IGF was found in patients with extrapancreatic tumour hypoglycaemia, but IGF binding was reduced. Immunoreactive IGF I was decreased in 5 of 10 patients. These results suggest that tumour hypoglycaemia in our patients is unlikely to be caused by increased IGF levels. 4) In patients with hyperprolactinaemia neither total IGF nor immunoreactive IGF I were elevated, and IGF binding was unchanged. 5) In newly detected insulin-deficient juvenile diabetics total IGF and immunoreactive IGF I levels were within the normal range, although the variation was greater than in normal subjects. However, IGF binding was markedly decreased.

Changes in bioactive IGF-I and IGF-binding protein-1 during an oral glucose tolerance test in patients with liver cirrhosis

“…In accordancew ith previous studies (15)(16)(17)(18), we confirmed that fasting serum concentrationso ft otal andf reeI GF-I were reduced and IGFBP-1 levels elevated,w hile IGF-I:IGFBP-1b inaryc omplex and IGFBP-2 tended to be higher in cirrhotic patients, as compared with healthycontrols. In addition,weshowed that thec irculatingb ioactivityo fI GF-I wasa lso subnormal in cirrhosis, and that levels, in contrast to totala nd free IGF-I, declined during the OGTT.T his observation mayb ee xplained by the higher levels of unsaturatedI GFBP-1 (i.e.…”

“…Indeed, in comparison to healthysubjects, increasedcirculating levels of IGFBP-1, but lower total and free IGF-I, are characteristic features of liver cirrhosis (15)(16)(17)(18)(19). Furthermore, one of the major health problems in cirrhotic patients is malnutrition, whichexertsaserious adverse effect on clinical outcome.…”

Soluble α-Klotho: a novel serum biomarker for the activity of GH-producing pituitary adenomas

“…Changes in IGFBP concentrations contribute to the limitations known for a variety of IGF1 assays (32). To circumvent these problems, we used a classical and time-consuming assay in which carrier proteins are removed before the samples are incubated with the antibodies (33,34).…”

Growth Failure in Cholestatic Rats: The Effect of Malnutrition on Insulin-Like Growth Factor I

“…Reductions in somatomedins measured by a variety of methods have been observed in studies on adults with chronic liver disease. Wu et al (6) initially demonstrated depressed somatomedins (measured as sulfation factor) in adults with cirrhosis, and this finding was confirmed in additional studies using radioreceptor assays (7) and RIA (8,9). Unfortunately, the role of malnutrition in these patients was not considered.…”