Serum Levels of Interleukin-4, Interleukin-10 and Interferon-γ in Patients with Chronic Hepatitis B Infection

Abstract

Background: The imbalance of T-helper (Th) lymphocyte cytokine production plays an important role in the immunopathogenesis of chronic Hepatitis B Virus (HBV) infection. Th1 cytokines are necessary for host antiviral immune response, while Th2 cytokines have an immunomodulatory role. Objectives: The current study aimed at determining the serum profile of Th1 and Th2 cytokines in patients with chronic HBV infection and to assess the correlation between the levels of the cytokines and ALT level in the patients. Methods: Sixty patients with chronic hepatitis B and 60 age and gender-matched healthy controls were enrolled in the study. The serum levels of Th1 cytokine, interferon gamma (IFN-γ), Th2 cytokines, IL-4, and IL-10 were measured using the enzyme linked immunosorbent assay (ELISA). The serum ALT level was measured by the Colorimetry method. Results: The results showed that the level of IL-4 was significantly lower in patients in comparison with the controls (P < 0.05). However, the level of IL-10 was not significantly different in patients and healthy individuals (P > 0.05). The concentration of IFN-γ was significantly higher in patients compared with the healthy controls (P < 0.05). No significant correlation was found between serum levels of IL-4, IL-10 and IFN-γ and nor was it found between the levels of these cytokines and serum ALT level. Conclusions: The results revealed enhanced Th1 response and reduced Th2 response, which is favored for the formation of a strong immune response and leads to viral elimination. The decrease of IL-4 indicated a lower viral load in the patients. In addition, a non-active disease resulted from the patients' IL10 level showing no significant difference between the patients and the controls. Significant increase of IFN-γ in patients indicated the activation of Th1 cells. The immunological outcome can help in finding better treatment strategies. The results suggest that interferon therapy with low dose can be a helpful strategy. Although the decrease in ALT level was not significantly correlated with an increase in IFN-γ in the patients, the increase of IFN-γ level demonstrated a modulation in hepatocellular damage.

Design and characterization of Lactotransferrin peptide-loaded dextran-docosahexaenoic acid nanoparticles: an immune modulator for hepatic damage

“…Additionally, because there was no discernible difference in the levels of IL10 between the patients and the controls, the condition was dormant. IFN- levels in patients significantly increased, which suggested that Th 1 cells were activated 38 . In the present investigation, apoptotic markers caspase-3, and caspase-9 were elevated in the hepatotoxic treatment group (Group 5).…”

Association between interferon-gamma (IFN-γ) gene polymorphisms (+874A/T and +2109A/G), and susceptibility to hepatitis B viral infection (HBV)

“…IFN-γ may affect the immune response of the HBV patients in light of its role in regulating and suppressing immune response (Ben-Ari et al, 2003;Dorman and Holland, 2000;Qi et al, 2005;Wai and Fontana, 2003). In chronic hepatitis B, the raised serum levels of interferon-α correlated with the presence of viral replication (Brunetto and Bonino, 2014;Hosseini Khorami et al, 2018).…”

Do CCR5 (CCR5Δ32) and TLR3 (RS5743313) gene polymorphisms prevent chronic hepatitis B infection?

“…It is well known that cytokines such as IFN‐ɣ and TNF‐alpha play a central role in the clearance of acute infection or the persistence of HBV 29 . Serum IFN‐ɣ levels are found to be higher in CHB patients in comparison to healthy controls and increased serum levels of IFN‐γ are correlated with HBV‐related active hepatitis 30,31 . To date, there is no study for investigation for relation of serum IFN‐ɣ levels of hepatitis B patients and CCR5 Δ32 and TLR3 (rs5743313) polymorphisms by comparison the immune and CHB groups.…”