Serum levels of protein S100B predict intracranial lesions in mild head injury

Cervellin, Gianfranco; Benatti, Mario; Carbucicchio, Andrea; Mattei, Loris; Cerasti, Davide; Aloe, Rosalia; Lippi, Giuseppe

doi:10.1016/j.clinbiochem.2012.01.006

Cited by 37 publications

(22 citation statements)

References 29 publications

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“…The peritraumatic amnesia was determined by the patient ' s ability to recall events they can remember immediately before and a er the injury. Inclusion criteria were the same as those described in a previous study [16] . Ethics approval for the collection of samples was obtained from the local committee.…”

Section: Serum Samples From Patients With Mtbimentioning

confidence: 99%

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Martínez‐Morillo¹,

Childs

García

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Brain injury is a medical emergency that needs to be diagnosed and treated promptly. Several proteins have been studied as biomarkers of this medical condition. The aims of this study were to: 1) evaluate the selectivity and precision of a commercial ELISA kit for neurofilament medium polypeptide (NFM) protein; and 2) evaluate the concentration in cerebrospinal fluid (CSF) and serum of healthy individuals and patients with brain damage.An ELISA from Elabscience was used. The selectivity was evaluated using size-exclusion chromatography and mass spectrometry. Intra- and inter-batch coefficients of variation (CV) were also studied. Fifty-one CSF samples from 36 age-matched patients with hemorrhagic stroke (HS) (n=30), ischemic stroke (IS) (n=11) and healthy individuals (n=10) were assayed. In addition, serum samples from healthy volunteers (n=47), 68 serum samples from seven patients with HS, 106 serum samples from 12 patients with traumatic brain injury (TBI) and 68 serum samples from 68 patients with mild traumatic brain injury (mTBI) were also analyzed.NFM was identified in the chromatographic fraction with highest immunoreactivity. The intra- and inter-batch CVs were ≤10% and ≤13%, respectively. The CSF-NFM concentration in HS was significantly higher (p<0.0001) than in IS and controls. Serum NFM concentration ranged from 0.26 to 8.57 ng/mL in healthy individuals (median=2.29), from 0.97 to 42.4 ng/mL in HS (median=10.8) and from 3.48 to 45.4 ng/mL in TBI (median=14.7). Finally, 44% of patients with mTBI had increased NFM concentration, with significantly higher levels (p=0.01) in patients with polytrauma.To our knowledge this is the first study describing increased NFM levels in CSF and serum from patients with brain damage.

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Section: Serum Samples From Patients With Mtbimentioning

confidence: 99%

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Martínez‐Morillo¹,

Childs

García

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The globally valuable diagnostic performance of MPV for identification of brain injury in patients with MHT (i.e. AUC of 0.74; P ¼ 0.001) also provides a reliable basis for planning further investigations to establish whether this parameter, alone or in combination with other promising biomarkers such as protein S100B [5], may be useful for diagnostic evaluation of MHT in the ED. Distribution of median and interquartile range (IQR) values of mean platelet volume (MPV) in patients with mild head trauma.…”

Section: Discussionmentioning

confidence: 96%

“…Although a kaleidoscope of serum biomarkers is now available for diagnostics of several human diseases, only a few were proven to have clinical usefulness in patients with neurological disorders so far. In particular, only the protein S-100B has shown clinical value in ruling out MHT patients without ICI, with potential saving of 20-30% computerized tomography scans [5].…”

Section: Introductionmentioning

confidence: 99%

The mean platelet volume is decreased in patients with mild head trauma and brain injury

Lippi

Carbucicchio

Benatti

et al. 2013

Blood Coagulation &Amp; Fibrinolysis

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We planned a prospective study to assess platelet number and size in patients with or without brain injury after mild head trauma (MHT). Platelet count and mean platelet volume (MPV) were assessed in consecutive patients admitted to the emergency department with isolate MHT, as well as in healthy blood donors who served as controls. The study population consisted in 54 patients with MHT, 13 of whom (24%) with intracranial lesions suggestive for brain injury, and 339 healthy blood donors. The value of platelet count was significantly lower in patients with MHT and positive computerized tomography than in healthy controls (P = 0.014). The vales of MPV progressively decreased from healthy controls (11.1 fl) to patients with MHT and negative computerized tomography (9.8 fl; P < 0.001), and further to patients with MHT and positive computerized tomography (8.6 fl; P < 0.001). The MPV was significantly lower in patients with MHT and positive computerized tomography than in those with negative computerized tomography (P = 0.002). As compared with healthy controls, the frequency of decreased MPV values was 10-fold and 17-fold higher in MTH patients with negative and positive computerized tomography, respectively. The MPV exhibited an area under the curve of 0.74 (95% CI, 0.58 to 0.89; P < 0.001) for differentiating MHT patients with positive computerized tomography from those with negative computerized tomography. MHT patients display a larger prevalence of small and hyporeactive platelets. This observation provides a reliable basis for planning further studies to establish whether MPV may be useful for diagnostic evaluation of MHT in the emergency department.

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“…Some biological markers of brain injury have recently been proposed, including protein S100B, neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), cleaved Tau protein (CTP), brain type fatty acid-binding protein (B-FABP), kallikrein 6 (KLK6), ubiquitin C-terminal hydrolase (UCH-L1) and II-spectrin breakdown product 145 kDa (SBDP145), among others. [6][7][8][9][10] Although encouraging results have been published about the potential usefulness of some of these biomarkers in research studies, especially protein S100B, 11,12 there is still limited clinical evidence to allow recommendation of routine implementation for diagnosis and management of patients with MHI. 13,14 Red blood cell (RBC) distribution width (RDW) is a measure of anysocytosis, which is commonly used in combination with mean corpuscular volume (MCV) for investigating the underlying cause(s) of anemia.…”

Section: Introductionmentioning

confidence: 99%

Red blood cell distribution width and erythrocyte parameters in patients with brain injury after mild head trauma

Lippi

Carbucicchio²,

Avanzini

et al. 2013

Emerg Care J

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This prospective study was planned to assess whether red blood cell (RBC) parameters may be useful in diagnostics of patients with brain injury after mild head trauma. The RBC count, hemoglobin, hematocrit, RBC distribution width (RDW) and mean corpuscular volume were assessed in all consecutive patients admitted to the emergency department over 3 months with isolate, mild head trauma and Glasgow coma scale between 14-15, and seen within 3 h from trauma. The final study population consisted of 54 patients (21 women and 33 men; median age=48 years), of whom, 13 (24%) with positive computed tomography (CT). No significant difference was found for age (P=0.45) and gender (P=0.21) distribution between CT positive and negative patients. No significant difference was observed for the median concentration of all the RBC parameters tested, and the prevalence of anemia (P=0.37) and anysocytosis (P=0.40) did not differ significantly between patients with positive and negative CT. Red blood cell distribution width assessment upon patient admission did not provide a significant contribution to final diagnosis of mild head injury in receiver operating characteristic curve analysis [area under the curve (AUC) 0.51; P=0.44]. We conclude that assessment of RDW does not provide useful clinical information for diagnosing brain injury after mild head trauma.

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Serum levels of protein S100B predict intracranial lesions in mild head injury

Cited by 37 publications

References 29 publications

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

The mean platelet volume is decreased in patients with mild head trauma and brain injury

Red blood cell distribution width and erythrocyte parameters in patients with brain injury after mild head trauma

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