Serum levels of testosterone, 11‐ketotestosterone and oestradiol‐17β in three species of sturgeon during gonadal development and final maturation induced by hormonal treatment

Abstract

Changes in serum concentrations of two androgens, testosterone (T) and 11-ketotestosterone (11KT), and oestradiol-17b (E2) in male and female giant sturgeon Huso huso, Russian sturgeon Acipenser gueldenstaedtii and stellate sturgeon Acipenser stellatus were studied at different stages of gonadal maturity and after final maturation induced by hormonal treatment. Both male and female fish displayed a distinct increase in serum steroid concentrations during gonadal development. 11KT levels were significantly higher in males than females, with a positive correlation detected between 11KT and T concentrations. In maturing males and females, higher values of both 11KT and T were observed in stellate sturgeon compared to giant and Russian sturgeons. Vitellogenesis and high E2 levels were correlated in maturing sturgeon females. # 2004 The Fisheries Society of the British Isles

Comparative Study of Sex Steroid Levels of Persian Sturgeon, Acipenser persicus Males in Responding Negative and Positive to LHRH-A2 Hormone

“…similar results were reported on same species where the levels of T and 11-KT increased after LH-RH-A injection [15]. Also, in another study on Great sturgeon, Huso huso L, Russian sturgeon and Stellate sturgeon males revealed that T and 11K levels have a increasing trends during maturation stages [16]. Previous researches shown that T and 11K are predominant androgen in males of teleost species and T acts a precursor of 11K and could be involved in spermatogenesis process [15,17].…”

“…Our findings, concerning steroid levels in Persian sturgeon males are in agreement with results reported by Semenkova et al (2002) [6] in Stellate sturgeon, Acipenser stellatus (Pallas) which, levels of T, 11K and P4 elevate after LHRH-A injection in spermiating males, whereas such elevations were not observed in non spermiating males. Also our data confirmed by Artyukhin et al (2006) [2] in Russian sturgeon, Acipenser gueldenstaedti (Brandt) and Barannikova et al (2004) in Sterlet, Acipenser ruthenus L. Possible reasons for the absence spermiation and low levels of steroids after hormonal stimulation were: (1) damage of steroidogenesis process (2) delay of the maturation and (3) not completed stage of the spermatogenesis [6,14]. On the other hand, Environmental pollution is one of the major factors which can disrupt the reproduction in fish at all gonadal stages [14].…”

EFFECT OF DIFFERENT DOSES OF SYNTHETIC HORMONE LHRH-A2 ON SERUM SEX HORMONES, OVULATION PERCENT AND EGG HATCHING RATES OF PERSIAN STURGEON Acipenser persicus

“…The glucose concentration was measured spectrophotometrically (Jenway, England) with a diagnostic kit (Pars Azmoon Co, Tehran, Iran). Steroids levels (Cortisol, Testosterone) and GTHII concentrations (ng/mL) were measured by Enzyme-Linked Immunosorbent Assay (ELISA) according to Barannikova et al ( , 2004 and Semenkova et al (2002). Assay kits were purchased from Immunotech Company, France.…”

Comparative analysis of milt quality and steroid levels in blood and seminal fluid of Persian sturgeon males, Acipenser persicus during final maturation induced by hormonal treatment

“…In another study, similar results were reported on same species where the concentrations of T,20β, increased after LH-RH-A treatment (Bayunova et al 2006). Also, the T and 11-KT levels showed increasing trends during gonadal development of giant sturgeon, Huso huso (L., 1758), Russian sturgeon, Acipenser gueldenstaedtii Brandt, 1833 and stellate males (Barannikova et al 2004). Cortisol (C) levels reflect generally the level of stress in fish, including sturgeons (Pickering 1989;Barton et al 1998;Belanger et al 2001;Bayunova et al 2002).…”