Serum Lipids Studied by Electrophoresis on Paper

Rosenberg, I. N.

doi:10.3181/00379727-80-19752

Cited by 43 publications

(8 citation statements)

References 4 publications

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“…In the Tiselius electrophoresis apparatus the presence of the pre-albumin peak might have been easily overlooked 2 This observation is at variance with a study reported from this laboratory (8) because of its fast migration, but it could be easily demonstrated on paper strips. Furthermore, the patterns on the paper strips represented specific color reactions, which gave also some information on the chemical nature of the separated fractions.…”

Section: Controlscontrasting

confidence: 56%

Effects of Heparin on Alimentary Hyperlipemia. An Electrophoretic Study 1

Herbst¹,

Hurley²

1954

J. Clin. Invest.

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A few recent communications deal with electrophoretic observations concerning the effect of heparin on alimentary hyperlipemia. Nikkila (1) found by moving boundary electrophoresis a decrease of beta-1 globulin and an increase in alpha-1 globulin after the injection of heparin. Rosenberg (2) and Fasoli (3) noted on paper electrophoresis an increase in the mobility of the beta lipoproteins which migrated in or near the alpha-2 globulin area. On the other hand, Spitzer (4) stated that administration of heparin does not alter the electrophoretic patterns of serum proteins in either fasting or lipemic dogs.To obtain further information about the effect of heparin on the plasma proteins in alimentary hyperlipemia normal individuals were given a meal rich in fat and thereafter the electrophoretic pattern of the serum was studied both before and after the intravenous injection of heparin. Electrophoretic analysis was made by paper electrophoresis as well as by moving boundary electrophoresis.The results of these studies are described in this paper. MATERIALS AND METHODSCollection of blood. Ten ml. of blood were withdrawn from eight normal individuals in a fasting state, after which a meal was given consisting of one pint of heavy cream, 16 Gm. of cocoa and 32 Gm. of sugar. Four and one-half hours after the ingestion of the meal, another 10 ml. of blood were withdrawn and subsequently 50 mg. of heparin (Lederle)'were injected intravenously. Fifteen minutes after the injection of heparin, 10 ml. of blood were taken again. The blood samples were allowed to clot at room temperature, then were centrifuged and the serum removed with a pipette.Analytical methods. For paper electrophoresis the method of Grassmann and Hannig (5) was used. Serum in the amount of 0.02 ml. was placed on a paper strip (Whatman No.

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Section: Controlscontrasting

confidence: 56%

Effects of Heparin on Alimentary Hyperlipemia. An Electrophoretic Study 1

Herbst¹,

Hurley²

1954

J. Clin. Invest.

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“…La révélation des lipoprotéines en électrophorèse libre est basée sur un procédé optique (Blix, Tiselius et Svensson, 1941). En électrophorèse de zone (sur papier, sur gel d'amidon, gel d'agarose ou acétate de cellulose) (Charman et Landowne, 1967), les lipoprotéines sont révélées par des colo rants spécifiques des graisses ; Soudan IV (Rosenberg, 1952) ou Scharlach, Soudan III, Noir Soudan (Swahn, 1952 ;Kunkel et Slater, 1952 ;Mac Donald, 1958 ; , Oil red O (Durrum, Paul et Smith, 1952, Jencke, Hyatt, Jetton, Mattingly et Durrum, 1956, Lees et Hatch, 1963 ; Lees et Frederickson, 1964). Au lieu de 2 constituants, Swahn (1953) croit pouvoir en distinguer 4 dans le sérum normal : le premier se situe entre l'albumine et les globulines alphaj et représente 30 % des lipoprotéines : il représente le groupe alpha ; un second peu important chez le sujet normal (3 % des lipo protéines) migre au niveau des alpha g-globulines : c'est la pré-beta-lipoprotéine de Lees et Fredrickson (1964) ; le troisième, représentant 60 % des LP est constitué par les beta-lipoprotéines ; le quatrième représente les chylomicrons dont la migration est inhibée par leur haute absorbabilité par le papier.…”

Section: ) Electrophorèseunclassified

Le Métabolisme Lipidique dans le Syndrome Néphrotique.

Malmendier¹,

Lambert²

1970

Ann Intern Med

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Thèse de doctorat/ PhD ThesisCitation APA: Malmendier, C. (1969 Cette thèse de doctorat a été numérisée par l'Université libre de Bruxelles. L'auteur qui s'opposerait à sa mise en ligne dans DI-fusion est invité à prendre contact avec l'Université (di-fusion@ulb.ac.be).Dans le cas où une version électronique native de la thèse existe, l'Université ne peut garantir que la présente version numérisée soit identique à la version électronique native, ni qu'elle soit la version officielle définitive de la thèse.DI-fusion, le Dépôt Institutionnel de l'Université libre de Bruxelles, recueille la production scientifique de l'Université, mise à disposition en libre accès autant que possible. Les oeuvres accessibles dans DI-fusion sont protégées par la législation belge relative aux droits d'auteur et aux droits voisins. Toute personne peut, sans avoir à demander l'autorisation de l'auteur ou de l'ayant-droit, à des fins d'usage privé ou à des fins d'illustration de l'enseignement ou de recherche scientifique, dans la mesure justifiée par le but non lucratif poursuivi, lire, télécharger ou reproduire sur papier ou sur tout autre support, les articles ou des fragments d'autres oeuvres, disponibles dans DI-fusion, pour autant que :Le nom des auteurs, le titre et la référence bibliographique complète soient cités; L'identifiant unique attribué aux métadonnées dans DI-fusion (permalink) soit indiqué;Le contenu ne soit pas modifié.

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“…Following injection of heparin in alimentary lipsemia there are also changes in the distribution of the plasma lipoproteins on paper electrophoresis [Rosenberg, 1952;Fasoli, Glassman, Magid and Foa, 1954;Dangerfield and Smith, 1954;Herbst and Hurley, 1954;Comfort, 1955]. The chylomicron component is diminished and there is an increase in the mobility of both the a-and ,B-lipoproteins.…”

Section: The Properties Of Clearing Factormentioning

confidence: 99%

Untitled

1957

Exp Physiol

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THE possibility that heparin or related chemical substances might be concerned in the transport of fat in the body was suggested by a chance observation made by Hahn in 1943. He noticed that when dogs with a gross alimentary lipwemia were transfused with blood containing heparin as the anticoagulant, samples of plasma withdrawn one to five minutes after the transfusion were completely clear. Hahn found that this clearing of alimentary lipaemia also occurred when animals were given intravenous injections of heparin; it did not occur when heparin was mixed with lipsemic plasma in vitro. Later, Anderson and Fawcett [1950] found that a clearing effect could be produced in vitro when plasma from an animal previously injected with heparin (heparinized plasma) was mixed with lipaemic plasma. These two phenomena, described as the heparin clearing reaction in vivo and in vitro have been confirmed in many animal species including birds and reptiles. The purpose of this review is to describe the investigation of these phenomena and to discuss their possible significance in fat transport. The reaction in vitro has received the most attention and will be discussed first. The events which occur in the lipaemic animal following the injection of heparin can then be considered in relation to the findings in vitro. THE HEPARIN CLEARING REACTION IN VITRO The Mechanism of ClearingThe turbidity of the plasma in alimentary lipawmia is due to the presence of large numbers of visible lipid particles of varying size. They include the chylomicra and a variety of low density lipoprotein complexes. These particles are composed primarily of triglyceride but also contain varying amounts of protein, phosphatide and cholesterol [Laurell, 1954;Robinson, 1955;Lindgren, Nichols and Freeman, 1955;Havel, Eder and Bragdon, 1955]. The experiments of Anderson and Fawcett [1950] showed that, following heparin injection, plasma contained a component, the so-called clearing factor, which had the property of changing these lipid complexes so that they no longer interfered with the transmission of light. This clearing property of heparinized plasma can be demonstrated in vitro in a number of ways: by mixing heparinized plasma with lipaemic plasma [Anderson and Fawcett, 1950], or by adding fatty chyle or isolated turbid lipoproteins to heparinized plasma [French, Robinson and Florey, 1953; Brown, Boyle 151

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Serum Lipids Studied by Electrophoresis on Paper

Cited by 43 publications

References 4 publications

Effects of Heparin on Alimentary Hyperlipemia. An Electrophoretic Study 1

Effects of Heparin on Alimentary Hyperlipemia. An Electrophoretic Study 1

Le Métabolisme Lipidique dans le Syndrome Néphrotique.

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