Serum Malassezia-specific IgE in dogs with recurrent Malassezia otitis externa without concurrent skin disease

Layne, Elizabeth A.; DeBoer, Douglas J.

doi:10.1016/j.vetimm.2016.05.008

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Since all questions were binary summed scores were tested for classification performance on the whole dataset of 4,111 dogs. Using the summed score on the entire population enabled us to isolate potential undiagnosed cases of atopic dermatitis (‘false positives’) from the group of dogs with ‘Other’ skin conditions creating four groups for comsparion 24 :Cases: dogs whose owners reported a veterinary diagnosis of “atopic dermatitis or atopy (environmental allergies, including mite allergies)”Controls were selected based upon the following criteria:No reported diagnosis of “atopic dermatitis or atopy (environmental allergies, including mite allergies)”No “current or past areas of abnormal skin (i.e. red, patchy, hairless, rough, swollen or discoloured)”No “Current or past signs of abnormal itchiness (frequent and recurrent rubbing, licking, chewing or scratching of the same areas)”Finally, dogs were excluded as a control if owners reported that the dog had an undiagnosed skin problem and the free text description mentioned allergies to plants, dust, household products, or seasonal itching.Potential Cases: dogs without a current veterinary diagnosis of canine atopic dermatitis that get classified as cases by the summed scoreOther: dogs with skin problems that were classified as a non-case by the summed score.…”

Section: Methodsmentioning

confidence: 99%

Development and validation of a new standardised data collection tool to aid in the diagnosis of canine skin allergies

Harvey

Shaw

Blott

et al. 2019

Sci Rep

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Canine atopic dermatitis (cAD) is a common hereditary clinical syndrome in domestic dogs with no definitive diagnostic tests, which causes marked morbidity and has a high economic impact internationally. We created a novel questionnaire for Labrador (LR) and Golden retriever (GR) owners to evaluate canine skin health with respect to clinical signs of cAD. 4,111 dogs had fully completed questionnaires (2,803 LR; 1,308 GR). ‘Cases’ (793) had a reported veterinary diagnosis of cAD, and ‘controls’ (1652) had no current or past clinical signs of cAD and were aged >3 years. Remaining dogs (1666) were initially categorised as ‘Other’. Simulated annealing was used comparing ‘Cases’ and ‘Others’ to select a novel set of features able to classify a known case. Two feature sets are proposed, one for use on first evaluation and one for dogs with a history of skin problems. A sum for each list when applied to the whole population (including controls) was able to classify ‘Cases’ with a sensitivity of 89% to 94% and specificity of 71% to 69%, respectively, and identify potentially undiagnosed cases. Our findings demonstrate for the first time that owner questionnaire data can be reliably used to aid in the diagnostic process of cAD.

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Section: Methodsmentioning

confidence: 99%

Development and validation of a new standardised data collection tool to aid in the diagnosis of canine skin allergies

Harvey

Shaw

Blott

et al. 2019

Sci Rep

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“…In dogs, concentrations of IgE antibodies to M. pachydermatis are significantly higher in atopic dogs than in healthy dogs or non‐atopic dogs with Malassezia overgrowth . However, dogs with recurrent Malassezia otitis had similar concentrations of allergen‐specific IgE to those with healthy ears, suggesting that hypersensitivity is not always involved in such infections . Using Western immunoblotting to characterise individual antigen responses, proteins with molecular weights of 45, 52, 56 and 63 kDa from M. pachydermatis have been demonstrated to be major allergens in atopic dogs with Malassezia overgrowth …”

Section: Pathogenesis: Immunological Responses To Malassezia Yeasts and Their Clinical Relevance In Diagnosis/therapymentioning

confidence: 99%

Biology, diagnosis and treatment ofMalasseziadermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology

Bond

Morris

Guillot

et al. 2020

Veterinary Dermatology

118

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Background -The genus Malassezia is comprised of a group of lipophilic yeasts that have evolved as skin commensals and opportunistic cutaneous pathogens of a variety of mammals and birds.Objectives -The objective of this document is to provide the veterinary community and other interested parties with current information on the ecology, pathophysiology, diagnosis, treatment and prevention of skin diseases associated with Malassezia yeasts in dogs and cats.Methods and material -The authors served as a Guideline Panel (GP) and reviewed the literature available prior to October 2018. The GP prepared a detailed literature review and made recommendations on selected topics. The World Association of Veterinary Dermatology (WAVD) Clinical Consensus Guideline committee provided guidance and oversight for this process. The document was presented at two international meetings of veterinary dermatology societies and one international mycology workshop; it was made available for comment on the WAVD website for a period of six months. Comments were shared with the GP electronically and responses incorporated into the final document.Conclusions and clinical importance -There has been a remarkable expansion of knowledge on Malassezia yeasts and their role in animal disease, particularly since the early 1990's. Malassezia dermatitis in dogs and cats has evolved from a disease of obscurity and controversy on its existence, to now being a routine diagnosis in general veterinary practice. Clinical signs are well recognised and diagnostic approaches are well developed. A range of topical and systemic therapies is known to be effective, especially when predisposing factors are identified and corrected.

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“…infections is generally associated by the emergence of primary diseases such as canine atopic dermatitis or combined with several predisposing factors (7). The host immune response to microorganisms in the external ear canal likely plays a pivotal role, but few data are available in dogs, except for studies on the immune reaction against Malassezia (8)(9)(10). Cerumen, or earwax, is a biological substance composed of lipids, proteins, amino acids, and carbohydrates produced by the combination of the excretions of ceruminous and sebaceous glands in the auditory canal of the external ear of mammalians.…”

Section: Introductionmentioning

confidence: 99%

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa

et al. 2020

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Otitis externa is one of the most common diseases in dogs. It is associated with bacteria and yeast, which are regarded as secondary causes. Cerumen is a biological substance playing an important role in the protection of ear skin. The involvement of cerumen in immune defense is poorly understood. MicroRNAs can modulate the host immune response and can provide promising biomarkers for several inflammatory and infectious disorder diagnosis. The aims of this study were to profile the cerumen miRNA signature associated with otitis externa in dogs, integrate miRNAs to their target genes related to immune functions, and investigate their potential use as biomarkers. Cerumen was collected from healthy and otitis affected dogs and the expression of miRNAs was profiled by Next Generation Sequencing; the validation of the altered miRNAs was performed using RT-qPCR. The potential ability of miRNAs to modulate immune-related genes was investigated using bioinformatics tools. The results pointed out that 32 miRNAs, of which 14 were up-and 18 down-regulated, were differentially expressed in healthy vs. otitis-affected dogs. These results were verified by RT-qPCR. To assess the diagnostic value of miRNAs, ROC analysis was carried out, highlighting that 4 miRNAs are potential biomarkers to discriminate otitis-affected dogs. Bioinformatics showed that cerumen miRNAs may be involved in the modulation of host immune response. In conclusion, we have demonstrated for the first time that miRNAs can be efficiently extracted and quantified from cerumen, that their profile changes between healthy and otitis affected dogs, and that they may serve as potential biomarkers. Further studies are necessary to confirm their diagnostic value and to investigate their interaction with immune-related genes.

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Serum Malassezia-specific IgE in dogs with recurrent Malassezia otitis externa without concurrent skin disease

Cited by 7 publications

References 27 publications

Development and validation of a new standardised data collection tool to aid in the diagnosis of canine skin allergies

Development and validation of a new standardised data collection tool to aid in the diagnosis of canine skin allergies

Biology, diagnosis and treatment ofMalasseziadermatitis in dogs and cats Clinical Consensus Guidelines of the World Association for Veterinary Dermatology

Identification of Altered miRNAs in Cerumen of Dogs Affected by Otitis Externa

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