Serum metabolomic analysis in positive-TgAb or positive-TPOAb in female Hashimoto's thyroiditis patients

Abstract

Background TgAb and TPOAb are effective and sensitive diagnose index for HT which is a common AID.TgAb and TPOAb present varying degrees correlatuion with different metabolites in different gender. However, the morbidity in female are higher than male in HT. The research in exploring the correction between metabolic and positive-TgAb or positive-TPOAb in female HT patients is still absent. Methods 14 healthy, 14 TPOAb(+), 4 TgAb(+) patients serum sample were included. Metabolites were detected using the LC-MS. Sstatistical analysis were performed by SPSS. PLS-DA and OPLS-DA were carried by SIMCA. VIP > 1.5 metabolites by OPLS-DA were assessed for statistical significance by t-test or non-parametric test. Enrichment analysis and heatmap of metabolite were conducted by MetaboAnalyst. Correlation analysis was performed by Origin 2021. The ROC curve was established by SPSS. Metabolite point plotting was drawn by Graph prism 9.0. Results Based on VIP > 1.5 and P < 0.05 as selection criteria, 36 metabolites were derived. 13 metabolites were selected from the control vs the TPOAb (+) group, 23 metabolites were identified from the control vs the TgAb (+) group. TgAb and Phenylacetyl-L-glutamine / TPOAb and LPC 16:0 sn-1 performed strong correlation the TPOAb (+) group. Furthermore, TPOAb and LPE 16:1 was presented correlation the TgAb (+) group. Enrichment analysis of metabolic pathways showed that Glycine, serine and threonine metabolism was significant in TPOAb (+), while Galactose metabolism in TgAb (+) group. Conclusion The level of serum metabolites in TPOAb(+) TgAb(-) female patients and TPOAb(-) TgAb(+) female patients are different.