Serum metabolomics analysis of rat after intragastric infusion of Pu‐erh theabrownin

Liu, Jian; Peng, Chunxiu; Gao, Bin; Gong, Jiashun

doi:10.1002/jsfa.7556

Cited by 22 publications

(19 citation statements)

References 30 publications

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“…However, poor bioavailability, unfavourable side effects, and high costs limited the development and clinical application of catechins . Apart from polyphenols, TB attracts increasing attention in recent years due to its bioactivities and therapeutic potential . In our previous studies, TB exerted strong pro‐apoptotic effects against non‐small cell lung cancer via the p53 signalling pathway .…”

Section: Discussionmentioning

confidence: 99%

Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway

Jin

Zhou

Yan

et al. 2018

J Cellular Molecular Medi

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Osteosarcoma becomes the second leading cause of cancer death in the younger population. Current outcomes of chemotherapy on osteosarcoma were unsatisfactory to date, demanding development of effective therapies. Tea is a commonly used beverage beneficial to human health. As a major component of tea, theabrownin has been reported to possess anti‐cancer activity. To evaluate its anti‐osteosarcoma effect, we established a xenograft model of zebrafish and employed U2OS cells for in vivo and in vitro assays. The animal data showed that TB significantly inhibited the tumour growth with stronger effect than that of chemotherapy. The cellular data confirmed that TB‐triggered DNA damage and induced apoptosis of U2OS cells by regulation of Mki67, PARP, caspase 3 and H2AX, and Western blot assay showed an activation of p53 signalling pathway. When P53 was knocked down by siRNA, the subsequent downstream signalling was blocked, indicating a p53‐dependent mechanism of TB on U2OS cells (p53 wt). Using osteosarcoma cell lines with p53 mutations (HOS, SAOS‐2 and MG63), we found that TB exerted stronger inhibitory effect on U2OS cells than that on p53‐mut cell lines, but it also exerted obvious effect on SAOS‐2 cells (p53 null), suggesting an activation of p53‐independent pathway in the p53‐null cells. Interestingly, theabrownin was found to have no toxicity on normal tissue in vivo and could even increase the viability of p53‐wt normal cells. In sum, theabrownin could trigger DNA damage and induce apoptosis on U2OS cells via a p53‐dependent mechanism, being a promising candidate for osteosarcoma therapy.

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Section: Discussionmentioning

confidence: 99%

Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway

Jin

Zhou

Yan

et al. 2018

J Cellular Molecular Medi

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“…Theabrownins (TB) are water‐soluble, macromolecular tea pigments derived from various phenolics, polysaccharides, proteins, lipids and caffeine via bioconversion by microbial polyphenol oxidase and peroxidase . TB exhibit diverse biological properties that combat atherosclerosis, diabetes and allergic responses . In addition, TB contribute to the brownish red color of tea infusion …”

Section: Introductionmentioning

confidence: 99%

High‐theabrownins instant dark tea product by Aspergillus niger via submerged fermentation: α‐glucosidase and pancreatic lipase inhibition and antioxidant activity

Wang

Zhang

et al. 2017

J Sci Food Agric

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The results of the present study show that careful management of culture conditions can produce a dark tea high in theabrownins. Furthermore, high-theabrownins instant dark tea could serve as a source of bioactive products and be used in functional foods as an ingredient imparting antioxidant properties and the ability to inhibit pancreatic lipase and α-glucosidase. © 2017 Society of Chemical Industry.

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“…TB fractionated from Pu‐erh tea have exhibited diverse bioactivities. For instance, in rat models they lower serum lipid levels, show antioxidative effects and induce weight loss (Gong et al ., ; Liu et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Evaluation of microbial toxins, trace elements and sensory properties of a high‐theabrownins instant Pu‐erh tea produced using Aspergillus tubingensis via submerged fermentation

Wang

Šarkanj

Jurasović

et al. 2018

Int J of Food Sci Tech

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Theabrownins (TB) are polymeric phenolic compounds associated with the multiple bioactivities of Pu-erh tea, a post-fermented Chinese dark tea. High-TB instant Pu-erh tea was produced via a novel submerged fermentation (SF) using Aspergillus tubingensis and compared with samples produced commercially via the conventional solid-state fermentation (SSF). Viable microorganisms and microbial toxins, especially aflatoxins B 1 , G 1 , B 2 , G 2 , cyclopiazonic acid, fumonisins B 1 , B 2 , B 3 and ochratoxin A, were below the detection limit in all samples. Fewer microbial metabolites were found in SF instant tea compared with the SSF teas. Based on an adult consuming 1 g of instant Pu-erh tea daily, the dietary intake of investigated elements was below the safe limits recommended by various authorities. Tasters viewed the instant tea infusions as very mild, smooth, mellow and full. This suggested that submerged fermentation using A. tubingensis offers a speedy and safe alternative to commercial production of instant Pu-erh tea.Safety of high-theabrownins instant Pu-erh tea Q. Wang et al.LOD, limit of detection;

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Serum metabolomics analysis of rat after intragastric infusion of Pu‐erh theabrownin

Cited by 22 publications

References 30 publications

Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway

Theabrownin triggers DNA damage to suppress human osteosarcoma U2OS cells by activating p53 signalling pathway

High‐theabrownins instant dark tea product by Aspergillus niger via submerged fermentation: α‐glucosidase and pancreatic lipase inhibition and antioxidant activity

Evaluation of microbial toxins, trace elements and sensory properties of a high‐theabrownins instant Pu‐erh tea produced using Aspergillus tubingensis via submerged fermentation

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