Serum Metabolomics Study Based on LC‐MS and Antihypertensive Effect of <i>Uncaria</i> on Spontaneously Hypertensive Rats

Liu, Ana; Chu, Yanjun; Wang, Xiaoming; Rao, Yu; Jiang, Haiqiang; Li, Yunlun; Zhou, Honglei; Gong, Lili; Yang, Wenqing; Ju, Jianqing

doi:10.1155/2018/9281946

Cited by 27 publications

(23 citation statements)

References 36 publications

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“…Several biomarkers were previously found in a metabolomics study by this research group. These biomarkers were mainly associated with lipid, vitamin and amino acid metabolism [2,3]. However, in these experimental studies of hypertension, changes in metabolites were measured at one time point, and dynamic monitoring was not performed.…”

Section: Introductionmentioning

confidence: 99%

Establishment of the circadian metabolic phenotype strategy in spontaneously hypertensive rats: a dynamic metabolomics study

Wang

et al. 2020

J Transl Med

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Background: Circadian rhythms play a fundamental role in the progression of cardiovascular events. Almost all cardiovascular diseases have a circadian misalignment usually characterized by changes in metabolites. This study aimed to dynamically monitor rhythmic biomarkers, to elucidate the metabolic pathways that are potentially under circadian control in spontaneously hypertensive rats (SHRs), and to eventually establish a circadian metabolic phenotype strategy based on metabolomics. Methods: In this study, an untargeted metabolomics technology was used to dynamically monitor changes in serum metabolites between SHR model group and WKY control group. Liquid chromatography-mass spectrometry (LC-MS) combined with multivariate statistical analysis was applied to identify markers of hypertension rhythm imbalance. The concentrations of amino acids and their metabolites identified as markers were quantified by a subsequent targeted metabolomics analysis. Overall, these approaches comprehensively explored the rhythm mechanism and established a circadian metabolic phenotype strategy. Results: The metabolic profile revealed a disorder in the diurnal metabolism pattern in SHRs. Moreover, multivariate statistical analysis revealed metabolic markers of rhythm homeostasis, such as arginine, proline, phenylalanine, citric acid, l-malic acid, succinic acid, etc., accompanied by an imbalance in hypertension. The key metabolic pathways related to rhythm imbalance in hypertension were found by enrichment analysis, including amino acid metabolism, and the tricarboxylic acid cycle (TCA). In addition, the quantitative analysis of amino acids and their metabolites showed that the changes in leucine, isoleucine, valine, taurine, serine, and glycine were the most obvious. Conclusions: In summary, this study illustrated the relationship between metabolites and the pathways across time on hypertension. These results may provide a theoretical basis for personalized treatment programmes and timing for hypertension.

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Section: Introductionmentioning

confidence: 99%

Establishment of the circadian metabolic phenotype strategy in spontaneously hypertensive rats: a dynamic metabolomics study

Wang

et al. 2020

J Transl Med

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“…Gastrodia elata can antagonize epinephrine, regulate blood vessels, and dilate small blood vessels, thereby regulating blood pressure. In addition, several clinical studies found that Uncaria could decrease BP effectively by regulating the above biomarkers and metabolic pathways [47]. e antihypertensive effect of Uncaria alkaloids is related to the decrease in frequency [48].…”

Section: Discussionmentioning

confidence: 99%

Randomized Controlled Trials of Tianma Gouteng Decoction Combined with Nifedipine in the Treatment of Primary Hypertension: A Systematic Review and Meta‐Analysis

Tai

Zou

Zhang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Hypertension is a primary risk factor for cardiovascular disease (CVD). Tianma Gouteng decoction (TGD), originating from Zabingzhengzhixinyi, has been used for thousands of years in China to treat hypertension, giddiness, and migraine. This updated meta-analysis aimed at assessing the efficacy and safety of TGD combined with nifedipine in the treatment of primary hypertension. Methods. Related research published prior to September 1, 2019, was found in electronic databases without language limitations. Fourteen studies were selected and analyzed for specified criteria, including the quality of the studies. All outcomes were recorded exhaustive. Data management and analysis were performed using RevMan 5.3 software. Results. A total of 1,537 (769 cases in the experimental group and 768 cases in the control group) patients were enrolled. The total efficacy rate was improved significantly for the combination of nifedipine with TGD compared to nifedipine treatment alone (I2 = 22%, RR = 1.17, and 95% CI: 1.12 to 1.22). Traditional Chinese medicine (TCM) symptoms of patients were obviously improved in the experimental group than in the control group (I2 = 44%, RR = 1.26, and 95% CI: 1.17 to 1.36). TGD combined with nifedipine shows a better effect than nifedipine in decreasing diastolic blood pressure (I2 = 95%, MD = −5.32, and 95% CI: −8.19 to −2.45) and systolic blood pressure (I2 = 98%, MD = −9.35, and 95% CI: −15.03 to −3.67) of patients. A sensitivity analysis was conducted for SBP and DBP by removing 2 studies and recalculated the combined estimate on remaining studies. The results of SBP showed a small heterogeneity (I2 = 17%, MD = −13.95, 95% CI: −14.86 to −13.05, and P<0.00001) when two studies (shicaihong 2017 and xiaoyugao 2017) were removed. And the results of DBP showed no heterogeneity (I2 = 0, MD = −8.36, 95% CI: −8.91 to −7.81, and P<0.00001) when two studies (panzhixiong 2019 and shicaihong 2017) were removed. Conclusion. The combination of TGD and nifedipine has a better effect in the treatment of hypertension, including blood pressure lowering and patients’ TCMs improving. However, our findings must be handled with care because of the small sample size and low quality of clinic trials cited. Other rigorous and large-scale RCTs are in need to confirm these results.

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“…CerS form dihydroceramides by condensing sphinganine and acyl-CoA, and they are a family of six isoforms (CerS1-6) that each display specificity for certain acyl-CoA chain lengths. Ceramides are formed from dihydroceramides by the action of the enzyme dihydroceramide desaturase ( 41 , 42 ).…”

Section: Introduction To Ceramidesmentioning

confidence: 99%

“…Both dihydroceramides and ceramides correlate with the release of the inflammatory cytokine interleukin 6 (IL-6), but only dihydroceramides correlates with macrophage inflammatory protein 1β (MIP-1β) release ( 13 , 15 ). Studies have also shown that dihydroceramide levels are elevated in patients with rheumatoid arthritis ( 109 ), patients with left ventricular assist devices ( 110 ) and hypertensive rats ( 13 – 15 , 41 , 111 , 112 ).…”

Section: Introduction To Ceramidesmentioning

confidence: 99%

The Role of Ceramides in Diabetes and Cardiovascular Disease Regulation of Ceramides by Adipokines

2020

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Metabolic dysfunction is intertwined with the pathophysiology of both diabetes and cardiovascular disease. Recently, one particular lipid class has been shown to influence the development and sustainment of these diseases: ceramides. As a subtype of sphingolipids, these species are particularly central to many sphingolipid pathways. Increased levels of ceramides are known to correlate with impaired cardiovascular and metabolic health. Furthermore, the interaction between ceramides and adipokines, most notably adiponectin and leptin, appears to play a role in the pathophysiology of these conditions. Adiponectin appears to counteract the detrimental effects of elevated ceramides, largely through activation of the ceramidase activity of its receptors. Elevated ceramides appear to worsen leptin resistance, which is an important phenomenon in the pathophysiology of obesity and metabolic syndrome.

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Serum Metabolomics Study Based on LC‐MS and Antihypertensive Effect of Uncaria on Spontaneously Hypertensive Rats

Cited by 27 publications

References 36 publications

Establishment of the circadian metabolic phenotype strategy in spontaneously hypertensive rats: a dynamic metabolomics study

Establishment of the circadian metabolic phenotype strategy in spontaneously hypertensive rats: a dynamic metabolomics study

Randomized Controlled Trials of Tianma Gouteng Decoction Combined with Nifedipine in the Treatment of Primary Hypertension: A Systematic Review and Meta‐Analysis

The Role of Ceramides in Diabetes and Cardiovascular Disease Regulation of Ceramides by Adipokines

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