Serum MicroRNA Expression Patterns in Subjects After the 5-km Exercise Are Strongly Associated With Cardiovascular Adaptation

Abstract

Circulating microRNAs (miRNAs) have been reported dysregulated during exercise. However, the changes of specific serum miRNAs during the 5-km run test with intensity of 51–52% maximum oxygen uptake (V̇O2max) and their association with traditional cardiovascular-related indicators remain well-characterized. Levels of miR-1, miR-21, miR-146a, miR-155, miR-181, and miR-210 were detected in 120 young subjects before and after the exercise training by quantitative reverse-transcription PCR (RT-qPCR). Besides, the levels of cardiac troponin I (cTNI), myoglobin (Myo), creatine kinase (CK), creatine kinase-MB (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), ischemia-modified albumin (IMA), interleukin-6 (IL-6), and C-reactive protein (CRP) were measured and the correlation between levels of serum miRNAs and biochemical parameters was also analyzed. Compared with resting state, the serum levels of miR-1, miR-146a, miR-155, miR-181, and miR-210 were significantly increased after exercise training. Serum levels of miR-146a, miR-155, and miR-210 after exercise training were positively correlated with Myo, CK-MB, and LDH, respectively, while miR-1, miR-146a, miR-181, and miR-155 were positively correlated with the levels of IL-6. Additionally, all the five miRNAs were negatively correlated with IMA levels. The multivariate logistic regression analysis showed that high levels of miR-146a, AST, LDH, and IL-6 in serum were risk factors, while low IMA contents were a protective factor for cardiovascular adaptation during exercise. In conclusion, the dynamic changes of miRNAs under the condition of the 5-km continuous running contribute to the adaptive regulation of the cardiovascular function of the body.

Identification of serum microRNA alterations associated with long-term exercise-induced motor improvements in patients with Parkinson disease

“…[29,30] It was reported that aerobic exercise training downregulated the cardiac levels of miR-181a, whereas circulating miR-181 was upregulated immediately after the exercise. [31][32][33] Some most significantly enriched pathways detected by KEGG Pathway analysis, such as the MAPK signaling pathway, the Wnt signaling pathway, and the Hippo pathway (Fig. 4), were reported to be closely related to the pathology of PD and/ or the differentially expressed miRNAs found in our study.…”

“…[29,30] It was reported that aerobic exercise training downregulated the cardiac levels of miR-181a, whereas circulating miR-181 was upregulated immediately after the exercise. [31–33]…”

Impact of Exercise and Aging on Mitochondrial Homeostasis in Skeletal Muscle: Roles of ROS and Epigenetics

“…IL-6 expression is also regulated by miRNAs. The highly expressed miR-1, miR-146a, miR-181, and miR-155 in plasma were positively correlated with IL-6 levels after exercise [193]. IL-6 is a direct target of miR-223-3p, which can directly bind the 3 untranslated region (UTR) of IL-6 and inhibit IL-6 expression [194,195].…”

Exercise-Induced miR-210 Promotes Cardiomyocyte Proliferation and Survival and Mediates Exercise-Induced Cardiac Protection against Ischemia/Reperfusion Injury

“…MiR-210 can facilitate cardiac repair by augmenting cardiomyocyte survival as well as angiogenesis and mitochondrial metabolism in animals [ 22 – 24 ]. Interestingly, miR-210 has been revealed to be regulated by exercise [ 25 , 26 ]. This study investigated the function and the mechanism of miR-210 in exercise-induced physiological cardiac growth and explored its involvement in exercise-driven cardiac protection against I/R injury.…”