Serum microRNAs as diagnostic markers of endometriosis: a comprehensive array-based analysis

Abstract

We identified several miRNAs in serum that distinguished subjects with endometriosis from those without. miR-125b-5p had the greatest potential as a single diagnostic biomarker. A combination of that miRNA with miR-451a and miR-3613-5p further improved diagnostic performance.

Overexpression of microRNA‐542‐3p attenuates the differentiating capacity of endometriotic stromal cells

“…The inverse relationship between the expression of miRNAs and their target genes is complex and evolving. There is accumulating data that miRNAs contribute to the pathogenesis of endometriosis by regulating abnormal cell differentiation, invasion, and inflammation 23. It has been reported that the expression of several miRNAs differs between the eutopic and ectopic endometrial tissues of women with and without endometriosis 9, 24.…”

The Importance of Standardization on Analyzing Circulating RNA

“…For example, let-7g and miR-221 levels showed a positive correlation with the progression of metabolic diseases in female patients [74]. Additionally, events like menstrual cycles [75–78] and pregnancy [79, 80] also altered the overall circulating miRNA profile. For example, the levels of miRNAs from a chromosome 14 and 19 miRNA clusters significantly increased during pregnancy [81].…”

The Role of Biomarkers in the Early Diagnosis of Endometriosis

“…In studying this process it has been revealed that microRNAs (miRNAs) are pivotal in this. MicroRNAs are intronic coded genes implicated in regulating gene expression and thus fundamentally control biological systems through gene silencing of several mRNAs simultaneously [14]. Through improvements in next generation sequencing and microarray technology we are now able to study the effects of miRNAs at a genomic scale.…”

“…Through improvements in next generation sequencing and microarray technology we are now able to study the effects of miRNAs at a genomic scale. Furthermore, the presence of circulating miRNA in the blood has led to research into their application as biomarkers in several conditions including sepsis and myocardial infarction [14]. Early research in this field has revealed differential miRNA expressions between endometrial patients and controls with downregulation of miR-34C-5p, miR-9 and mir-34b, let-7d etc.…”

“…Early research in this field has revealed differential miRNA expressions between endometrial patients and controls with downregulation of miR-34C-5p, miR-9 and mir-34b, let-7d etc. Let-7d was the first miRNA discovered to promote cellular differentiation with reduced expression of let-7 family members well known to be associated with malignancy which has shown to have in endometriosis sensitivity and specificity of 83.3% and 100% respectively [14]. Understandably the downregulation of a miRNA leads to a rise of its corresponding…”