Serum miR-200c and miR-371-5p as the Useful Diagnostic Biomarkers and Therapeutic Targets in Kawasaki Disease

Abstract

Kawasaki disease (KD) has complexly clinical features and laboratory parameters and there is no definitive biomarker for this disease and the therapy of KD also is complex and uncertain. In this study, 102 KD patients and 80 healthy controls were enrolled in this study and the serum microRNAs were detected by qRT-PCR. The results showed that, compared with KD patients with a good response to high-dose intravenous immunoglobulin (IVIG) therapy, serum miR-200c and miR-371-5p were significantly higher in KD patients with no response to IVIG therapy; compared with KD patients not needing plasma exchange, these two microRNAs were also significantly higher in KD patients needing plasma exchange. In addition, combination of serum miR-200c and miR-371-5p reflected obvious separation between KD patients and healthy controls or between KD patients with no response to IVIG therapy and KD patients with good response to IVIG therapy or KD patients needing plasma exchange and KD patients not needing plasma exchange. Finally, both serum miR-200c and miR-371-5p were also significantly lower in KD under different kinds of therapy. Therefore, serum miR-200c and miR-371-5p have ability as the useful diagnostic biomarkers and therapeutic targets in Kawasaki disease.

Diagnostic significance of circulating miRNAs in Kawasaki disease in China

“…In the included studies, the sample types of 3 studies were plasma, [ 25 – 27 ] and the remaining 3 studies were serum. [ 28 – 30 ] Besides, 3 studies defined healthy children as healthy controls (HCs), [ 26 , 27 , 29 ] 1 study defined juvenile systemic lupus erythematosus as the control, [ 25 ] 2 studies used both healthy and febrile children as controls. [ 28 , 30 ] Meanwhile, there were 2 types of reference miRNA, cel-miR-39, [ 25 , 27 , 28 , 30 ] and U6.…”

“…[ 28 , 30 ] Meanwhile, there were 2 types of reference miRNA, cel-miR-39, [ 25 , 27 , 28 , 30 ] and U6. [ 26 , 29 ] The basic characteristics of the included studies were shown in Table 1 .…”

Lack of association between miR-218 rs11134527 A>G and Kawasaki disease susceptibility

“…Rong et al [ 36 ] demonstrated that the serum miR-92a-3p levels were significantly higher in children with KD compared with non-Kawasaki disease subjects, and the miR-92a-3p level had a diagnostic sensitivity of 81.8% for KD. Zhang et al [ 37 ] enrolled 102 patients with KD and 80 healthy controls in a study and found that the serum miR-200c and miR-371-5p levels were significantly higher in the KD patients compared with the controls. These two miRNAs were significantly higher in KD patients who were resistant to IVIG compared with those for whom IVIG was effective; thus, these two miRNAs may serve as diagnostic biomarkers and therapeutic targets in KD.…”

An update on the role of epigenetics in systemic vasculitis

“…miR-200c promotes endothelial cell apoptosis, inducing vascular smooth muscle cell inflammatory response and modulating TLR4 response [52]. Both miR-200c and -371-5p were shown to effectively distinguish between KD and healthy controls as well as IVIG-responsive and nonresponsive patients [53]. More recent research has identified new miRNAs with disease activity that potentially target vessel endothelial cell functions in KD patients [54–58].…”