Serum miR-29a/b expression in gestational diabetes mellitus and its influence on prognosis evaluation

Abstract

Objective To evaluate serum microRNA (miR)-29a/b expression in gestational diabetes mellitus (GDM) and its influence on neonatal prognosis. Methods This was a retrospective study including 68 pregnant women with GDM (GDM group) and 55 healthy pregnant women of similar age range and gestation period (healthy group). Results The area under the curve was 0.829 for the diagnosis of GDM using serum miR-29a expression, 0.857 for diagnosis using serum miR-29b expression, and 0.944 for combined diagnosis (using both miR-29a and miR-29b). The fasting insulin (FINS) level of the GDM group was significantly lower than that of the healthy group; levels of fasting plasma glucose (FPG), 2-h plasma glucose (2hPG), and glycated hemoglobin (HbA1c) were significantly higher in the GDM group than in the healthy group. Both miR-29a and miR-29b were positively correlated with FINS levels and negatively correlated with FPG, 2hPG, and HbA1c levels. Serum miR-29a/b expression in pregnant women with GDM was not correlated with neonatal weight, premature delivery, or asphyxia but was correlated with pathologic jaundice. Conclusions Serum miR-29a/b expression was downregulated in pregnant women with GDM and correlated with neonatal pathologic jaundice, showing good individual (miR-29a or miR-29b) diagnostic value and excellent combined (miR-29a and miR-29b) diagnostic value.

Epigenetic Alterations Related to Gestational Diabetes Mellitus

“…This suggests that miR-29a is a negative regulator of serum glucose. A recent study supports the observation that GDM is characterized by lower circulating levels of miR-29a [71].…”

Emerging roles of exosomal miRNAs in diabetes mellitus

“…Interestingly, exosomal miR-NAs are closely associated with the gender difference of DM. Deng et al 163 found that serum exosomal miR-29a and miR-29b displayed the diagnostic values for the pregnant women with GDM, and ROC curve analysis indicated that miR-29a combined with miR-29b (the AUC value was 0.944 [95% CI: 0.907-0.982]) for the diagnosis was prior than the single indicator (the AUC values of miR-29a and miR-29b were 0.829 [95% CI: 0.755-0.903] and 0.857 [95% CI: 0.787-0.926]). Thus, exosomal miRNAs are promising molecular biomarkers for the screening and monitoring of DM.…”

Estimation of Glycated Hemoglobin A (HbA1c) at time of Pregnancy Diagnosis as a discriminative Early test for Women vulnerable to develop Gestational Diabetes Mellitus at the 24th Gestational Week

“…Moreover, T0 levels of HbA1c were significantly higher in GDM women in comparison to Non-GDM women. Similarly, multiple studies detected significantly higher HbA1c levels in women who developed GDM in comparison to those who completed their pregnancy free of GDM manifestations [20,21,22] . Statistical analyses defined high T0 levels of HbA1c and high ΔHbA1c as early specific discriminators for women vulnerable to development of GDM at the 24-26 th GW and paired-sample area analysis for AUC for T0 level of HbA1c and ΔHbA1c showed non-significant difference in favor of T0 level of HbA1c, so that T0 level of HbA1c could be considered as the significant early predictor for upcoming GDM, which spares time till the 12 th GW before institution of prophylactic and/or therapeutic measures.…”