Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

Xu, Huanxi; Yang, Yuqi; Zhao, Hongmei; Yang, Xiaoyu; Luo, Yukun; Ren, Yinxiang; Liu, Wei; Li, Ning

doi:10.1007/s13277-015-3514-z

Cited by 73 publications

(49 citation statements)

References 15 publications

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“…Xu et al () found that miR‐483 expression was significantly increased in OSCC patients, which was significantly correlated with tumor nodal metastasis (TNM) stage. And multivariate analyses for overall survival revealed that high miR‐483 expression was an independent prognostic factor for OSCC (Xu et al, ). Down‐regulation of miR‐22 has been observed in the development of many types of cancer (Damavandi et al, ; Yang et al, ; Zhang, Xia, Tian, Liu, & Zhou, ; Zuo et al, ), and overexpression of miR‐22 suppressed the proliferation, migration and invasion of gastric cancer and renal carcinoma cells (Zhang, Zhang et al, ; Zuo et al, ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3

Feng

Luo

Han

et al. 2018

Journal Cellular Physiology

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MicroRNAs (miRNAs) have been implicated as important regulators of carcinogenesis and tumor development. Recently, microRNA-22 (miR-22) has been reported to be a cancer-related miRNA in several types of tumors. In this study, we aimed to investigate the role of miR-22 in oral squamous cell carcinoma (OSCC). We found that miR-22 expression was significantly decreased in OSCC tissues compared with that in the adjacent noncancerous tissues. Furthermore, lentivirus-mediated miR-22 overexpression markedly reduced OSCC cell viability, migration and invasion, whereas miR-22 inhibitor promoted these parameters. Mechanistically, NLR family pyrin domain containing three (NLRP3) was identified as a direct target of miR-22. miR-22 expression was inversely correlated with NLRP3 expression both in OSCC tissues and cell lines. Moreover, overexpression of miR-22 in OSCC cells could reverse the tumor-promoting effect of the activated NLRP3 inflammasome and vice versus. Therefore, our results indicate that miR-22 may play a suppressive role in OSCC by targeting NLRP3, which offer new insights into the molecular mechanisms of the growth and metastasis of OSCC.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‐22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3

Feng

Luo

Han

et al. 2018

Journal Cellular Physiology

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“…And U6 snRNA (small nuclear RNA) was used as an internal control. All miRNAs were measured by using the equation

2^{- Δ {normalC}_{normalt}} false(normalΔ C_{t} = C_{m i normalRNA} - C_{t 0.6em normalU 0.6em 6} false)

.…”

Section: Methodsmentioning

confidence: 99%

“…And U6 snRNA (small nuclear RNA) was used as an internal control. All miRNAs were measured by using the equation 2 −∆C t (∆C t = C tmiRNA -C tU6 ) [13].…”

Section: Quantitative Real-time Pcr Verification Of Mirnasmentioning

confidence: 99%

The role of microRNA‐93 regulating angiopoietin2 in the formation of malignant pleural effusion

et al. 2017

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The biological roles of miRNAs in the development of malignant pleural effusion (MPE) are unclear. In this study, the miRNA microarray analysis was performed in two different prognosis groups of lung adenocarcinoma patients. Expression profiles of miRNAs in MPEs were identified. With the help of quantification PCR, we confirmed the expression differences of miRNAs and further analyzed their biological functions and relative target genes in vitro. The target gene of miR‐93 was estimated by online database, and also, the protein was tested. The target gene and the binding sites of specific miRNA were estimated by online database. The combining capacity of binding sites was verified by luciferase reporter gene assay, and the target gene protein was tested by western blot. We detected 107 miRNAs with expression differences (n = 10) and confirmed significant expression differences in miR‐93 and miR‐146a in two groups of patients (n = 84). By manipulating miR‐93 expression of human lymphatic endothelial cells (HLEC) and human umbilical vein endothelial cells (HUVEC), we discovered that high expression of miR‐93 inhibited migration, proliferation, and angiogenesis. And also, miR‐93 increased not only apoptosis, but also G1 phase cell block. By using luciferase reporter gene assay and western blot, we confirmed that angiopoietin2 (Ang2) was the target of miR‐93. The data showed that miR‐93 has an inhibiting effect on pleural effusion. By targeting Ang2, miR‐93 regulates angiogenesis and lymphangiogenesis and plays a role in pathogenetic mechanism of MPE. MiR‐93/Ang2 may shed light on potential new targets in cancer treatment.

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“…Recently, miR-483-3p has been reported to be involved in the occurrence of many diseases, such as esophageal squamous cell carcinoma (19), cardiomyocyte apoptosis (20) and gastric cancer (21). It has also been shown to be dysregulated and associated with poorer disease-specific survival in various cancers (22)(23)(24)(25)(26). Although Song et al (24) reported that miR-483-5p can serve as a negative regulator of lung cancer metastasis suppressors RhoGDI1 and ALCAM, the role of miR-483 in lung disease particularly COPD and the molecular mechanisms by which miR-483 regulates such diseases are still not clear.…”

Section: Discussionmentioning

confidence: 99%

miR-483-5p plays a protective role in chronic obstructive pulmonary disease

Shen

Tang

Guo

et al. 2017

International Journal of Molecular Medicine

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Altered microRNA (miRNA or miR) expression has been reported in chronic obstructive pulmonary disease (COPD). The present study aimed to identify the involvement of miRNAs in the pathophysiology of COPD and to explore the effects of various miRNAs with significant alteration on COPD in vitro. We conducted high‑throughput analysis of miRNAs (miRNA microarray) in lung samples from 10 COPD patients and 10 healthy persons with a validation experiment using quantitative (real‑time) polymerase chain reaction (real‑time PCR) panels. By analyzing 3,000 miRNAs in lung samples using a microarray, we identified 341 differentially expressed miRNAs (138 with high expression and 203 with low expression) in patients with COPD in comparison with the healthy controls. Then 15 high-expression candidates and 15 low-expression candidates with at least 2‑fold difference and P<0.05 were selected randomly to validate the changes in three independent experiments in vitro using real‑time PCR. The validation test showed a positive correlation with the microarray results. Then we chose miR‑483‑5p as our target. The effect of miR‑483‑5p on cell proliferation and expression of COPD-related proteins were detected using Cell Counting Kit 8 and western blot analysis, respectively. The results showed that miR‑483‑5p, which was significantly downregulated in COPD samples, abrogated the transforming growth factor‑β (TGF‑β)‑mediated decrease in cell proliferation, and increase in α‑smooth muscle actin (α‑SMA) and fibronectin expression in pulmonary epithelial and lung fibroblast cell lines, BEAS‑2B and HFL1. These findings suggest that miR‑483‑5p may play an important and protective role in patients with COPD and may serve as a useful biomarker and for early detection of COPD as well as a potential therapeutic tool.

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Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

Cited by 73 publications

References 15 publications

MicroRNA‐22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3

MicroRNA‐22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3

The role of microRNA‐93 regulating angiopoietin2 in the formation of malignant pleural effusion

miR-483-5p plays a protective role in chronic obstructive pulmonary disease

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