Serum Peptidome Patterns of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry Analysis

Abstract

Background. Colorectal cancer (CRC) is one of the most common cancers in the world, identification of biomarkers for early detection of CRC represents a relevant target. The present study aims to determine serum peptidome patterns for CRC diagnosis. Methods. The present work focused on serum proteomic analysis of 32 health volunteers and 38 CRC by ClinProt Kit combined with mass spectrometry. This approach allowed the construction of a peptide patterns able to differentiate the studied populations. An independent group of serum (including 33 health volunteers, 34 CRC, 16 colorectal adenoma, 36 esophageal carcinoma, and 31 gastric carcinoma samples) was used to verify the diagnostic and differential diagnostic capability of the peptidome patterns blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results. A quick classifier algorithm was used to construct the peptidome patterns for identification of CRC from controls. Two of the identified peaks at m/z 741 and 7772 were used to construct peptidome patterns, achieving an accuracy close to 100% (>CEA, P < 0.05). Furthermore, the peptidome patterns could differentiate validation group with high accuracy. Conclusions. These results suggest that the ClinProt Kit combined with mass spectrometry yields significantly higher accuracy for the diagnosis and differential diagnosis of CRC.

Serum peptidome profiling for the diagnosis of colorectal cancer: discovery and validation in two independent cohorts

“…The progress of tumor's malignancy is accompanied by alterations in exoproteases activities, affecting the constitution of endogenous peptides that can indicate the presence/absence of cancer [ 19 ]. The profiling of the serum peptidome has been used for the diagnosis of CRC in several studies [ 20 – 30 ]. Fan et al (2006) recruited 72 CRC patients and 65 healthy controls and randomly divided them into two groups: a model construction group and a validation group [ 20 ].…”

“…The profiling of the serum peptidome has been used for the diagnosis of CRC in several studies [ 20 – 30 ]. Fan et al (2006) recruited 72 CRC patients and 65 healthy controls and randomly divided them into two groups: a model construction group and a validation group [ 20 ]. They established a diagnostic model with two peptides that yielded a sensitivity of 94.74% and a specificity of 100% in the model construction phase and a sensitivity of 94.12% and a specificity of 100% in the validation phase [ 20 ].…”

Assessment of molecular differentiation in FFPE colon adenocarcinoma tissues using PCA analysis of MALDI IMS spectral data

“…Nowadays, the effort continues regarding the various components of the extracellular and intracellular tumor microenvironment that contribute to the colorectal cancer phenotype [21][22][23][24][25]. MALDI-TOF MS has been used in combination with separation techniques for serum proteomic profiling and early diagnosis of colorectal cancer [26][27][28]. Comparison of the proteome changes between tumour and neighbouring normal colorectal mucosa is a common methodology employed to identify proteins that have altered abundance associated with tumorigenesis [29].…”

Magnetic Bead-Based Serum Peptidome Profiling in Patients with Gestational Diabetes Mellitus

“…MALDL-TOF MS combined with WCX-MB is a promising diagnostic and has been successfully applied to cancer diagnosis [ 8 – 11 ]; however, few studies using this technique investigated the discriminating protein profiles in the serum of women with GDM. Our study compared peptide expression profiles of serum samples from patients with and without GDM by MALDI-TOF MS-based peptidome analysis in combination with WCX magnetic beads, aiming to identify a panel of differentially expressed specific peptides to discuss possible causes and facilitate the early diagnosis of GDM.…”